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ZCCHC4 Antibody (PA5-87672) in WB
Western blot analysis of ZCCHC4 in mouse kidney. Samples were incubated with ZCCHC4 Polyclonal antibody (Product # PA5-87672) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-87672
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 50-220 of human ZCCHC4 (NP_0792122)
Conjugate
Form
Liquid
Concentration
1.35 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2804337
Product Specific Information
Immunogen sequence: KVTQGKEETR RFYACSACRD RKDCNFFQWE DEKLSGARLA AREAHNRRCQ PPLSRTQCVE RYLKFIELPL TQRKFCQTCQ QLLLPDDWGQ HSEHQVLGNV SITQLRRPSQ LLYPLENKKT NAQYLFADRS CQFLVDLLSA LGFRRVLCVG TPRLHELIKL TASGDKKSNI K; Positive Samples: Mouse kidney
Target Information
The ZCCHC4 gene encodes zinc finger CCHC domain-containing protein 4, a member of the zinc finger CCHC-type (ZCCHC) superfamily characterized by a consensus sequence C-X2-C-X4-H-X4-C. This gene plays a vital role in binding single-stranded nucleic acids, particularly RNAs, and is involved in multiple cellular processes. Notably, ZCCHC4 has been implicated in cancer, with aberrantly high expression linked to poor prognosis and chemoresistance in various cancers, including hepatocellular carcinoma, pancreatic cancer, colon cancer, and esophageal cancer. The protein exhibits RNA-modifying capabilities, influencing cell growth and survival. Recent studies have identified the structural mechanisms through which ZCCHC4 interacts with RNA substrates, offering insights into its role in regulating cancer progression and therapeutic resistance. In esophageal cancer, downregulation of ZCCHC4 may enhance sensitivity to chemotherapeutic agents like cisplatin, indicating its potential as a target for cancer treatment strategies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: rRNA N(6)-adenosine-methyltransferase ZCCHC4; Zinc finger CCHC domain-containing protein 4
Gene Aliases: 4930449I23Rik; Zcchc4
UniProt ID: (Mouse) Q8BKW4
Entrez Gene ID: (Mouse) 78796
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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