Proteintech

ZCCHC6 Polyclonal Antibody

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ZCCHC6 Antibody (25196-1-AP) in WB

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 25196-1-AP (ZCCHC6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

ZCCHC6 Antibody in Immunoprecipitation (IP)

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Product Details

25196-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 11 publications 11 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ZCCHC6 Fusion Protein Ag18240 (1-251 aa encoded by BC032456)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.29 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MGDTAKPYF VKRTKDRGTM DDDDFRRGHP QQDYLIIDDH AKGHGSKMEK GLQKKKITPG NYGNTPRKGP CAVSSNPYAF KNPIYSQPAW MNDSHKDQSK RWLSDEHTGN SDNWREFKPG PRIPVINRQR KDSFQENEDG YRWQDTRGCR TVRRLFHKDL TSLETTSEME AGSPENKKQR SRPRKPRKTR NEENEQDGDL EGPVIDESVL STKELLGLQQ AEERLKRDCI DRLKRRPRNY PTAKYTCRLC DV (1-251 aa encoded by BC032456)

Target Information

Uridylyltransferase that mediates the terminal uridylation of mRNAs with short (less than 25 nucleotides) poly(A) tails, hence facilitating global mRNA decay (PubMed:19703396, PubMed:25480299). Essential for both oocyte maturation and fertility. Through 3' terminal uridylation of mRNA, sculpts, with TUT7, the maternal transcriptome by eliminating transcripts during oocyte growth. Involved in microRNA (miRNA)-induced gene silencing through uridylation of deadenylated miRNA targets (PubMed:25480299). Also functions as an integral regulator of microRNA biogenesiS using 3 different uridylation mechanisms (PubMed:25979828). Acts as a suppressor of miRNA biogenesis by mediating the terminal uridylation of some miRNA precursors, including that of let-7 (pre-let-7). Uridylated pre-let-7 RNA is not processed by Dicer and undergo degradation. Pre-let-7 uridylation is strongly enhanced in the presence of LIN28A (PubMed:22898984). In the absence of LIN28A, TUT7 and TUT4 monouridylate group II pre-miRNAs, which includes most of pre-let7 members, that shapes an optimal 3' end overhang for efficient processing (PubMed:25979828, PubMed:28671666). Add oligo-U tails to truncated pre-miRNAS with a 5' overhang which may promote rapid degradation of non-functional pre-miRNA species (PubMed:25979828). Does not play a role in replication-dependent histone mRNA degradation (PubMed:18172165). Due to functional redundancy between TUT4 and TUT7, the identification of the specific role of each of these proteins is difficult (PubMed:25979828, PubMed:25480299, PubMed:19703396, PubMed:22898984, PubMed:18172165, PubMed:28671666). TUT4 and TUT7 restrict retrotransposition of long interspersed element-1 (LINE-1) in cooperation with MOV10 counteracting the RNA chaperonne activity of L1RE1. TUT7 uridylates LINE-1 mRNAs in the cytoplasm which inhibits initiation of reverse transcription once in the nucleus, whereas uridylation by TUT4 destabilizes mRNAs in cytoplasmic ribonucleoprotein granules (PubMed:30122351). [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

RNA uridyl transferases TUT4/7 differentially regulate miRNA variants depending on the cancer cell type.

RNA (New York, N.Y.)

Medhi R,Price J,Furlan G,Gorges B,Sapetschnig A,Miska EA

Tue Mar 01 00:00:00 UTC 2022

TENT2, TUT4, and TUT7 selectively regulate miRNA sequence and abundance.

Nature communications

Yang A,Bofill-De Ros X,Stanton R,Shao TJ,Villanueva P,Gu S

Wed Sep 07 00:00:00 UTC 2022

TENT2, TUT4, and TUT7 selectively regulate miRNA sequence and abundance.

Nature communications

Yang A,Bofill-De Ros X,Stanton R,Shao TJ,Villanueva P,Gu S

Wed Sep 07 00:00:00 UTC 2022

Identification of RNA-binding proteins that partner with Lin28a to regulate Dnmt3a expression.

Scientific reports

Parisi S,Castaldo D,Piscitelli S,D'Ambrosio C,Divisato G,Passaro F,Avolio R,Castellucci A,Gianfico P,Masullo M,Scaloni A,Russo T

Wed Jan 27 00:00:00 UTC 2021

MiRNA post-transcriptional modification dynamics in T cell activation.

iScience

Rodríguez-Galán A,Dosil SG,Gómez MJ,Fernández-Delgado I,Fernández-Messina L,Sánchez-Cabo F,Sánchez-Madrid F

Fri Jun 25 00:00:00 UTC 2021

The molecular basis of coupling between poly(A)-tail length and translational efficiency.

eLife

Xiang K,Bartel DP

Fri Jul 02 00:00:00 UTC 2021

A Mechanism for microRNA Arm Switching Regulated by Uridylation.

Molecular cell

Kim H,Kim J,Yu S,Lee YY,Park J,Choi RJ,Yoon SJ,Kang SG,Kim VN

Thu Jun 18 00:00:00 UTC 2020

Critical Roles of Translation Initiation and RNA Uridylation in Endogenous Retroviral Expression and Neural Differentiation in Pluripotent Stem Cells.

Cell reports

Takahashi K,Jeong D,Wang S,Narita M,Jin X,Iwasaki M,Perli SD,Conklin BR,Yamanaka S

Tue Jun 02 00:00:00 UTC 2020

AGO-bound mature miRNAs are oligouridylated by TUTs and subsequently degraded by DIS3L2.

Nature communications

Yang A,Shao TJ,Bofill-De Ros X,Lian C,Villanueva P,Dai L,Gu S

Tue Jun 02 00:00:00 UTC 2020

AGO-bound mature miRNAs are oligouridylated by TUTs and subsequently degraded by DIS3L2.

Nature communications

Yang A,Shao TJ,Bofill-De Ros X,Lian C,Villanueva P,Dai L,Gu S

Tue Jun 02 00:00:00 UTC 2020

Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis.

Molecular cell

Marmor-Kollet H,Siany A,Kedersha N,Knafo N,Rivkin N,Danino YM,Moens TG,Olender T,Sheban D,Cohen N,Dadosh T,Addadi Y,Ravid R,Eitan C,Toth Cohen B,Hofmann S,Riggs CL,Advani VM,Higginbottom A,Cooper-Knock J,Hanna JH,Merbl Y,Van Den Bosch L,Anderson P,Ivanov P,Geiger T,Hornstein E

Thu Dec 03 00:00:00 UTC 2020

3' Uridylation Confers miRNAs with Non-canonical Target Repertoires.

Molecular cell

Yang A,Bofill-De Ros X,Shao TJ,Jiang M,Li K,Villanueva P,Dai L,Gu S

Thu Aug 08 00:00:00 UTC 2019

The RNA uridyltransferase Zcchc6 is expressed in macrophages and impacts innate immune responses.

PloS one

Kozlowski E,Wasserman GA,Morgan M,O'Carroll D,Ramirez NP,Gummuluru S,Rah JY,Gower AC,Ieong M,Quinton LJ,Mizgerd JP,Jones MR

Mon Oct 02 00:00:00 UTC 2017

The RNA uridyltransferase Zcchc6 is expressed in macrophages and impacts innate immune responses.

PloS one

Kozlowski E,Wasserman GA,Morgan M,O'Carroll D,Ramirez NP,Gummuluru S,Rah JY,Gower AC,Ieong M,Quinton LJ,Mizgerd JP,Jones MR

Mon Oct 02 00:00:00 UTC 2017

3' Uridylation controls mature microRNA turnover during CD4 T-cell activation.

RNA (New York, N.Y.)

Gutiérrez-Vázquez C,Enright AJ,Rodríguez-Galán A,Pérez-García A,Collier P,Jones MR,Benes V,Mizgerd JP,Mittelbrunn M,Ramiro AR,Sánchez-Madrid F

Thu Jun 01 00:00:00 UTC 2017

TUT7 catalyzes the uridylation of the 3' end for rapid degradation of histone mRNA.

RNA (New York, N.Y.)

Lackey PE,Welch JD,Marzluff WF

Tue Nov 01 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: FLJ13409; PAP associated domain containing 6; Terminal uridylyltransferase 7; TUTase 7; TUTase7; Zinc finger CCHC domain-containing protein 6; zinc finger, CCHC domain containing 6

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Gene Aliases: 6030448M23Rik; AA420405; HS2; KIAA1711; PAPD6; TUT7; ZCCHC6

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UniProt ID: (Human) Q5VYS8, (Mouse) Q5BLK4

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Entrez Gene ID: (Human) 79670, (Mouse) 214290, (Rat) 501515

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Function(s)

zinc ion binding poly(A) RNA binding RNA uridylyltransferase activity nucleic acid binding transferase activity nucleotidyltransferase activity metal ion binding

Process(es)

RNA 3'-end processing histone mRNA catabolic process

Performance Guarantee

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ZCCHC6 Polyclonal Antibody

ZCCHC6 Antibody (25196-1-AP) in WB

Product Details

25196-1-AP

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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