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          • Primary Antibodies ›
          • ZEB1 Antibodies

          Proteintech

          ZEB1 Polyclonal Antibody

          213 References
          View all (45) ZEB1 antibodies

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          Cite ZEB1 Polyclonal Antibody

          • Antibody Testing Data (10)
          ZEB1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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          ZEB1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 10

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          ZEB1 Antibody (21544-1-AP) in IHC (P)

          Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 21544-1-AP ( ZEB1 Antibody) at dilution of 1:500 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          ZEB1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          ZEB1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          ZEB1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          ZEB1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          ZEB1 Antibody in Western Blot (WB)
          ZEB1 Antibody in Western Blot (WB)
          ZEB1 Antibody in Western Blot (WB)
          ZEB1 Antibody in Western Blot (WB)
          ZEB1 Antibody in Western Blot (WB)
          ZEB1 Antibody in Immunoprecipitation (IP)

          Product Details

          21544-1-AP

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2400
          View 167 publications 167 publications

          Immunohistochemistry (IHC)

          -
          View 20 publications 20 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:500
          -

          Immunocytochemistry (ICC/IF)

          -
          View 14 publications 14 publications

          Immunoprecipitation (IP)

          0.5-4.0 µg
          View 4 publications 4 publications

          ChIP assay (ChIP)

          Assay-dependent
          View 8 publications 8 publications
          Product Specifications

          Species Reactivity

          Human, Mouse

          Published species

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          ZEB1 Fusion Protein Ag16204 (391-740 aa encoded by BC112392)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.27 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice (domestic); Dry ice (international)

          Product Specific Information

          The calculated molecular weight of ZEB1 is 124 kDa, but the post phosphorylation of protein is about 190-210 kDa. This is a rabbit polyclonal antibody raised against part of the full length of ZEB1 of human.

          Immunogen sequence: MVQAVVLPT VGLVSPISIN LSDIQNVLKV AVDGNVIRQV LENNQANLAS KEQETINASP IQQGGHSVIS AISLPLVDQD GTTKIIINYS LEQPSQLQVV PQNLKKENPV ATNSCKSEKL PEDLTVKSEK DKSFEGGVND STCLLCDDCP GDINALPELK HYDLKQPTQP PPLPAAEAEK PESSVSSATG DGNLSPSQPP LKNLLSLLKA YYALNAQPSA EELSKIADSV NLPLDVVKKW FEKMQAGQIS VQSSEPSSPE PGKVNIPAKN NDQPQSANAN EPQDSTVNLQ SPLKMTNSPV LPVGSTTNGS RSSTPSPSPL NLSSSRNTQG YLYTAEGAQE EPQVEPLDLS L (391-740 aa encoded by BC112392)

          Target Information

          ZEB1 (Zinc finger E-box-binding homeobox 1) acts as a transcriptional repressor. It inhibits interleukin-2 (IL-2) gene expression. ZEB1 enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and depending on the cell type. It represses E-cadherin promoters and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. ZEB1 also represses BCL6 transcription in the presence of the corepressor CTBP1. It postively regulates neuronal differentiation and represses RCOR1 transcription activation during neurogenesis. Mutations in the gene can result in corneal dystrophy, posterior polymorphous and corneal dystrophy, Fuchs endothelial 6.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Delta EF1; delta-crystallin enhancer binding factor 1; DELTA-EF1; MEB1; MGC133261; Negative regulator of IL2; NIL-2-A; NIL-2-A zinc finger protein; NIL-2A; OTTHUMP00000019405; posterior polymorphous corneal dystrophy 3; RP11-472N13.4; TCF-8; Transcription factor 8; transcription factor 8 (represses interleukin 2 expression); twirler; Zinc finger E-box-binding homeobox 1; Zinc finger homeobox protein 1a; zinc finger homeodomain enhancer-binding protein

          View more View less

          Gene Aliases: 3110032K11Rik; [delta]EF1; AREB6; BZP; DELTAEF1; FECD6; MEB1; Nil2; NIL2A; PPCD3; TCF-8; Tcf18; TCF8; Tw; ZEB; ZEB1; ZFHEP; ZFHX1A; Zfx1a; Zfx1ha

          View more View less

          UniProt ID: (Human) P37275, (Mouse) Q64318

          View more View less

          Entrez Gene ID: (Human) 6935, (Mouse) 21417

          View more View less

          Function(s)
          transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription coactivator activity transcription corepressor activity protein binding transcription factor binding zinc ion binding E-box binding nucleic acid binding DNA binding double-stranded DNA binding sequence-specific DNA binding metal ion binding
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter pattern specification process nervous system development central nervous system development negative regulation of cell proliferation regulation of mesenchymal cell proliferation regulation of transforming growth factor beta receptor signaling pathway cell differentiation negative regulation of epithelial cell differentiation regulation of T cell differentiation in thymus positive regulation of neuron differentiation negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter animal organ development embryonic camera-type eye morphogenesis embryonic morphogenesis embryonic skeletal system morphogenesis semicircular canal morphogenesis regulation of smooth muscle cell differentiation cartilage development cellular response to amino acid stimulus cochlea morphogenesis regulation of transcription from RNA polymerase II promoter immune response cell proliferation
          It has to be done as per old AB suggested Products section.
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