Proteintech

ZEB1 Monoclonal Antibody (1H1F1)

ZEB1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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ZEB1 Antibody (66279-1-IG) in IHC (P)

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 66279-1-IG ( ZEB1 Antibody) at dilution of 1:400 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

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Product Details

66279-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:8,000

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:800

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1H1F1

Immunogen

ZEB1 Fusion Protein Ag17118

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Target Information

ZEB1 (Zinc finger E-box-binding homeobox 1) acts as a transcriptional repressor. It inhibits interleukin-2 (IL-2) gene expression. ZEB1 enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and depending on the cell type. It represses E-cadherin promoters and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. ZEB1 also represses BCL6 transcription in the presence of the corepressor CTBP1. It postively regulates neuronal differentiation and represses RCOR1 transcription activation during neurogenesis. Mutations in the gene can result in corneal dystrophy, posterior polymorphous and corneal dystrophy, Fuchs endothelial 6.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The aryl sulfonamide indisulam inhibits gastric cancer cell migration by promoting the ubiquitination and degradation of the transcription factor ZEB1.

The Journal of biological chemistry

Lu J,Li D,Jiang H,Li Y,Lu C,Chen T,Wang Y,Wang X,Sun W,Pu Z,Qiao C,Ma J,Xu G

Sat Apr 01 00:00:00 UTC 2023

Loss of exosomal miR-200b-3p from hypoxia cancer-associated fibroblasts promotes tumorigenesis and reduces sensitivity to 5-Flourouracil in colorectal cancer via upregulation of ZEB1 and E2F3.

Cancer gene therapy

Gong W,Guo Y,Yuan H,Chai R,Wan Z,Zheng B,Hu X,Chen B,Gao S,Dai Q,Yu P,Tu S

Thu Jun 01 00:00:00 UTC 2023

Hepatic stellate cell-released CXCL1 aggravates HCC malignant behaviors through the MIR4435-2HG/miR-506-3p/TGFB1 axis.

Cancer science

Li S,Hu X,Yu S,Yi P,Chen R,Huang Z,Huang Y,Huang Y,Zhou R,Fan X

Wed Feb 01 00:00:00 UTC 2023

Overexpression of SERPINA3 promotes tumor invasion and migration, epithelial-mesenchymal-transition in triple-negative breast cancer cells.

Breast cancer (Tokyo, Japan)

Zhang Y,Tian J,Qu C,Peng Y,Lei J,Li K,Zong B,Sun L,Liu S

Thu Jul 01 00:00:00 UTC 2021

The calcium pump PMCA4 prevents epithelial-mesenchymal transition by inhibiting NFATc1-ZEB1 pathway in gastric cancer.

Biochimica et biophysica acta. Molecular cell research

Wang T,Li N,Jin L,Qi X,Zhang C,Hua D

Tue Dec 01 00:00:00 UTC 2020

A Mutation in ZNF143 as a Novel Candidate Gene for Endothelial Corneal Dystrophy.

Journal of clinical medicine

Kim Y,You HJ,Park SH,Kim MS,Chae H,Park J,Jekarl DW,Kim J,Kwon A,Choi H,Kim Y,Paek AR,Lee A,Kim JM,Park SY,Kim Y,Joo K,Jung J,Chung SH,Mok JW,Kim M

Tue Aug 06 00:00:00 UTC 2019

Bioinformatics

Protein Aliases: delta-crystallin enhancer binding factor 1; DELTA-EF1; MGC133261; Negative regulator of IL2; NIL-2-A; NIL-2-A zinc finger protein; NIL-2A; OTTHUMP00000019405; posterior polymorphous corneal dystrophy 3; RP11-472N13.4; TCF-8; Transcription factor 8; transcription factor 8 (represses interleukin 2 expression); Zinc finger E-box-binding homeobox 1; zinc finger homeodomain enhancer-binding protein

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Gene Aliases: AREB6; BZP; DELTAEF1; FECD6; NIL2A; PPCD3; TCF8; ZEB1; ZFHEP; ZFHX1A

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UniProt ID: (Human) P37275

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Entrez Gene ID: (Human) 6935

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Function(s)

transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription coactivator activity transcription corepressor activity protein binding transcription factor binding zinc ion binding E-box binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated regulation of transcription from RNA polymerase II promoter immune response pattern specification process central nervous system development cell proliferation negative regulation of cell proliferation regulation of mesenchymal cell proliferation regulation of transforming growth factor beta receptor signaling pathway negative regulation of epithelial cell differentiation regulation of T cell differentiation in thymus positive regulation of neuron differentiation negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter embryonic camera-type eye morphogenesis embryonic skeletal system morphogenesis semicircular canal morphogenesis regulation of smooth muscle cell differentiation cartilage development cellular response to amino acid stimulus cochlea morphogenesis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ZEB1 Monoclonal Antibody (1H1F1)

ZEB1 Antibody (66279-1-IG) in IHC (P)

Product Details

66279-1-IG

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Target Information

Bioinformatics

Performance Guarantee

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