Invitrogen

ZEB1 Monoclonal Antibody (3G6), eBioscience™

ZEB1 Antibody in Immunocytochemistry (ICC/IF)

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ZEB1 Antibody (14-9741-82) in ICC/IF

Immunofluorescence analysis of ZEB1 was performed using 70% confluent log phase MDA-MB-231 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with ZEB1 Monoclonal Antibody (3G6), eBioscience (Product # 14-9741-80) at 0.5 µg/mL dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Flu... View More

ZEB1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ZEB1 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

ZEB1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of ZEB1 was observed in MDA-MB-231 but not in MCF7 which is known to have low expression (DOI:10.1242/jcs.084194) using Anti-ZEB1 Monoclonal Antibody (3G6), eBioscience (Product # 14-9741-80) in Western Blot. {RE}

Product Details

14-9741-82

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

10 µg/mL

Immunocytochemistry (ICC/IF)

0.5 µg/mL

ChIP assay (ChIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

3G6

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2572970

Product Specific Information

Description: The monoclonal antibody 3G6 recognizes human, mouse, and rat ZEB1. ZEB1, or zinc finger E-box binding homeobox 1, is also known as Transcription Factor 8 (TCF8), Negative Regulator of IL2, and AREB6. ZEB1 acts as a transcriptional repressor that inhibits interleukin-2 (IL-2) gene expression. ZEB1 also regulates expression of the genes E-cadherin, BCL6, ATP1A1, and RCOR1 through binding to multiple enhancer boxes (E-boxes). ZEB1 promotes epithelial mesenchymal transition (EMT) and invasion of various types of cancers, such as colorectal, breast, pancreatic, lung, and gallbladder. In normal tissues, ZEB1 is expressed in tissues undergoing differentiation such as T cell and skeletal differentiation. Mutations in ZEB1 have been associated with corneal dystrophy.

Applications Reported: This 3G6 antibody has been reported for use in western blotting, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.

Applications Tested: This 3G6 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue using high pH antigen retrieval and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

ZEB1 (Zinc finger E-box-binding homeobox 1) acts as a transcriptional repressor. It inhibits interleukin-2 (IL-2) gene expression. ZEB1 enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and depending on the cell type. It represses E-cadherin promoters and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. ZEB1 also represses BCL6 transcription in the presence of the corepressor CTBP1. It postively regulates neuronal differentiation and represses RCOR1 transcription activation during neurogenesis. Mutations in the gene can result in corneal dystrophy, posterior polymorphous and corneal dystrophy, Fuchs endothelial 6.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Stromal heterogeneity may explain increased incidence of metaplastic breast cancer in women of African descent.

Nature communications

Kumar B,Khatpe AS,Guanglong J,Batic K,Bhat-Nakshatri P,Granatir MM,Addison RJ,Szymanski M,Baldridge LA,Temm CJ,Sandusky G,Althouse SK,Cote ML,Miller KD,Storniolo AM,Nakshatri H

14-9741-82 was used in Immunohistochemistry to demonstrate that these cells have properties of fibroadipogenic/mesenchymal stromal cells that express PROCR and PDGFRα and transdifferentiate into adipogenic and osteogenic lineages.

Thu Sep 14 00:00:00 UTC 2023

Stromal heterogeneity may explain increased incidence of metaplastic breast cancer in women of African descent.

Nature communications

Kumar B,Khatpe AS,Guanglong J,Batic K,Bhat-Nakshatri P,Granatir MM,Addison RJ,Szymanski M,Baldridge LA,Temm CJ,Sandusky G,Althouse SK,Cote ML,Miller KD,Storniolo AM,Nakshatri H

Thu Sep 14 00:00:00 UTC 2023

Downregulation of long noncoding RNA HCP5/miR-216a-5p/ZEB1 axis inhibits the malignant biological function of laryngeal squamous cell carcinoma cells.

Frontiers in immunology

Zhang S,Huangfu H,Zhao Q,Li Y,Wu L

Published figure using ZEB1 monoclonal antibody (Product # 14-9741-82) in Western Blot

Tue Oct 18 00:00:00 UTC 2022

MicroRNA-200c/429 mediated regulation of Zeb1 augments N-Cadherin in mouse cardiac mesenchymal cells.

Cell biology international

Nath AV,Ajit S,Sekar AJ,P R AK,Muthusamy S

Published figure using ZEB1 monoclonal antibody (Product # 14-9741-82) in Western Blot

Tue Feb 01 00:00:00 UTC 2022

SPARC Induces E-Cadherin Repression and Enhances Cell Migration through Integrin αvβ3 and the Transcription Factor ZEB1 in Prostate Cancer Cells.

International journal of molecular sciences

López-Moncada F,Torres MJ,Lavanderos B,Cerda O,Castellón EA,Contreras HR

14-9741-82 was used in Western Blotting to conclude that SPARC induces E-cadherin repression and enhances PCa cell migration through the integrin αvβ3/ZEB1 signaling pathway.

Tue May 24 00:00:00 UTC 2022

SPARC Induces E-Cadherin Repression and Enhances Cell Migration through Integrin αvβ3 and the Transcription Factor ZEB1 in Prostate Cancer Cells.

International journal of molecular sciences

López-Moncada F,Torres MJ,Lavanderos B,Cerda O,Castellón EA,Contreras HR

14-9741-82 was used in Western Blotting to conclude that SPARC induces E-cadherin repression and enhances PCa cell migration through the integrin αvβ3/ZEB1 signaling pathway.

Tue May 24 00:00:00 UTC 2022

A Peninsular Structure Coordinates Asynchronous Differentiation with Morphogenesis to Generate Pancreatic Islets.

Cell

Sharon N,Chawla R,Mueller J,Vanderhooft J,Whitehorn LJ,Rosenthal B,Gürtler M,Estanboulieh RR,Shvartsman D,Gifford DK,Trapnell C,Melton D

14-9741-82 was used in Immunohistochemistry to find islet morphology and endocrine differentiation to be directly related.

Thu Feb 07 00:00:00 UTC 2019

Genetic Ancestry-dependent Differences in Breast Cancer-induced Field Defects in the Tumor-adjacent Normal Breast.

Clinical cancer research : an official journal of the American Association for Cancer Research

Nakshatri H,Kumar B,Burney HN,Cox ML,Jacobsen M,Sandusky GE,D'Souza-Schorey C,Storniolo AMV

14-9741 was used in Immunohistochemistry to utilize ancestry-mapped true normal breast tissues as controls to identify cancer-induced field defects in normal tissue adjacent to breast tumors in women of African American and European ancestry.

Wed May 01 00:00:00 UTC 2019

Secreted protein acidic and rich in cysteine (SPARC) induces epithelial-mesenchymal transition, enhancing migration and invasion, and is associated with high Gleason score in prostate cancer.

Asian journal of andrology

López-Moncada F,Torres MJ,Castellón EA,Contreras HR

Published figure using ZEB1 monoclonal antibody (Product # 14-9741-82) in Western Blot

Mon Aug 17 00:00:00 UTC 2020

A reciprocal repression between ZEB1 and members of the miR-200 family promotes EMT and invasion in cancer cells.

EMBO reports

Burk U,Schubert J,Wellner U,Schmalhofer O,Vincan E,Spaderna S,Brabletz T

Published figure using ZEB1 monoclonal antibody (Product # 14-9741-82) in ChIP assay

Sun Jun 01 00:00:00 UTC 2008

Bioinformatics

Protein Aliases: Delta EF1; delta-crystallin enhancer binding factor 1; DELTA-EF1; MEB1; MGC133261; Negative regulator of IL2; NIL-2-A; NIL-2-A zinc finger protein; NIL-2A; OTTHUMP00000019405; posterior polymorphous corneal dystrophy 3; RP11-472N13.4; TCF-8; Transcription factor 8; transcription factor 8 (represses interleukin 2 expression); twirler; Zfhep; Zinc finger E-box-binding homeobox 1; Zinc finger homeobox protein 1a; Zinc finger homeodomain enhancer-binding protein

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Gene Aliases: 3110032K11Rik; [delta]EF1; AREB6; BZP; DELTAEF1; FECD6; MEB1; Nil2; NIL2A; PPCD3; TCF-8; Tcf18; TCF8; Tw; ZEB; ZEB1; ZFHEP; ZFHX1A; Zfx1a; Zfx1ha

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UniProt ID: (Human) P37275, (Mouse) Q64318, (Rat) Q62947

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Entrez Gene ID: (Human) 6935, (Mouse) 21417, (Rat) 25705

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Function(s)

transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription coactivator activity transcription corepressor activity protein binding transcription factor binding zinc ion binding E-box binding nucleic acid binding DNA binding double-stranded DNA binding sequence-specific DNA binding metal ion binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter pattern specification process nervous system development central nervous system development negative regulation of cell proliferation regulation of mesenchymal cell proliferation regulation of transforming growth factor beta receptor signaling pathway cell differentiation negative regulation of epithelial cell differentiation regulation of T cell differentiation in thymus positive regulation of neuron differentiation negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter animal organ development embryonic camera-type eye morphogenesis embryonic morphogenesis embryonic skeletal system morphogenesis semicircular canal morphogenesis regulation of smooth muscle cell differentiation cartilage development cellular response to amino acid stimulus cochlea morphogenesis regulation of transcription from RNA polymerase II promoter immune response cell proliferation response to activity forebrain development response to nutrient levels cellular response to transforming growth factor beta stimulus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ZEB1 Monoclonal Antibody (3G6), eBioscience™

ZEB1 Antibody (14-9741-82) in ICC/IF

Product Details

14-9741-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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