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ZHX2 Polyclonal Antibody

ZHX2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 8

ZHX2 Antibody (20136-1-AP) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 20136-1-AP (ZHX2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

ZHX2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ZHX2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

20136-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunocytochemistry (ICC/IF)

1:50

Immunoprecipitation (IP)

0.5-4.0 µg

ChIP assay (ChIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ZHX2 Fusion Protein Ag14088 (488-837 aa encoded by BC042145)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.36 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: SDHRYRCQR GIVHITSESL AKDQLAIAAS RHGRTYHAYP DFAPQKFKEK TQGQVKILED SFLKSSFPTQ AELDRLRVET KLSRREIDSW FSERRKLRDS MEQAVLDSMG SGKKGQDVGA PNGALSRLDQ LSGAQLTSSL PSPSPAIAKS QEQVHLLRST FARTQWPTPQ EYDQLAAKTG LVRTEIVRWF KENRCLLKTG TVKWMEQYQH QPMADDHGYD AVARKATKPM AESPKNGGDV VPQYYKDPKK LCEEDLEKLV TRVKVGSEPA KDCLPAKPSE ATSDRSEGSS RDGQGSDENE ESSVVDYVEV TVGEEDAISD RSDSWSQAAA EGVSELAESD SDCVPAEAGQ A (488-837 aa encoded by BC042145)

Target Information

Zinc-fingers and homeobox (ZHX) proteins are transcription factors that interact with the activation domain of the A subunit of nuclear factor-Y (NF-YA). ZHX1-3 are ubiquitously expressed proteins expressed in various tissues. They act as transcriptional repressors and localize to the nucleus. The ZHX proteins contain two Cys(2)-His(2)-type zinc-finger motifs and five homeodomains (HDs). These domains allow the ZHX proteins to form homodimers, but they can also form heterodimers with each other. However, this dimerization is not required for repressor activity. Hypermethylation-mediated silencing of ZHX2 is an epigenetic event involved in hepatocellular carcinoma (HCC).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Zhx2 maintains islet β-cell mass and function by transcriptionally regulating Pax6.

iScience

Ding L,Zhang Y,Wang Y,Wang Y,Tong Z,Li P,Chen C,Wang B,Yue X,Li C,Wu Z,Liang X,Ma C,Gao L

Fri Jun 16 00:00:00 UTC 2023

Zhx2 maintains islet β-cell mass and function by transcriptionally regulating Pax6.

iScience

Ding L,Zhang Y,Wang Y,Wang Y,Tong Z,Li P,Chen C,Wang B,Yue X,Li C,Wu Z,Liang X,Ma C,Gao L

Fri Jun 16 00:00:00 UTC 2023

Zhx2 maintains islet β-cell mass and function by transcriptionally regulating Pax6.

iScience

Ding L,Zhang Y,Wang Y,Wang Y,Tong Z,Li P,Chen C,Wang B,Yue X,Li C,Wu Z,Liang X,Ma C,Gao L

Fri Jun 16 00:00:00 UTC 2023

ZHX2 inhibits thyroid cancer metastasis through transcriptional inhibition of S100A14.

Cancer cell international

Zhang Y,Sun M,Gao L,Liang X,Ma C,Lu J,Yue X

Sat Feb 12 00:00:00 UTC 2022

ZHX2 inhibits thyroid cancer metastasis through transcriptional inhibition of S100A14.

Cancer cell international

Zhang Y,Sun M,Gao L,Liang X,Ma C,Lu J,Yue X

Sat Feb 12 00:00:00 UTC 2022

Design, synthesis, and biological evaluation of novel dual inhibitors of heat shock protein 90/mammalian target of rapamycin (Hsp90/mTOR) against bladder cancer cells.

European journal of medicinal chemistry

Pan Z,Chen Y,Pang H,Wang X,Zhang Y,Xie X,He G

Tue Nov 15 00:00:00 UTC 2022

Upregulation of ZHX2 predicts poor prognosis and is correlated with immune infiltration in gastric cancer.

FEBS open bio

Cheng A,Guo X,Dai X,Wang Z

Tue Jun 01 00:00:00 UTC 2021

Upregulation of ZHX2 predicts poor prognosis and is correlated with immune infiltration in gastric cancer.

FEBS open bio

Cheng A,Guo X,Dai X,Wang Z

Tue Jun 01 00:00:00 UTC 2021

ZHX2 inhibits SREBP1c-mediated de novo lipogenesis in hepatocellular carcinoma via miR-24-3p.

The Journal of pathology

Yu X,Lin Q,Wu Z,Zhang Y,Wang T,Zhao S,Song X,Chen C,Wang Z,Xu L,Li C,Gao L,Liang X,Yue X,Ma C

Tue Dec 01 00:00:00 UTC 2020

Zhx2 Accelerates Sepsis by Promoting Macrophage Glycolysis via Pfkfb3.

Journal of immunology (Baltimore, Md. : 1950)

Wang Z,Kong L,Tan S,Zhang Y,Song X,Wang T,Lin Q,Wu Z,Xiang P,Li C,Gao L,Liang X,Ma C

Wed Apr 15 00:00:00 UTC 2020

HBV suppresses ZHX2 expression to promote proliferation of HCC through miR-155 activation.

International journal of cancer

Song X,Tan S,Wu Z,Xu L,Wang Z,Xu Y,Wang T,Gao C,Gong Y,Liang X,Gao L,Spear BT,Ma C

Sat Dec 15 00:00:00 UTC 2018

HBV suppresses ZHX2 expression to promote proliferation of HCC through miR-155 activation.

International journal of cancer

Song X,Tan S,Wu Z,Xu L,Wang Z,Xu Y,Wang T,Gao C,Gong Y,Liang X,Gao L,Spear BT,Ma C

Sat Dec 15 00:00:00 UTC 2018

Proliferation and osteo/odontogenic differentiation of stem cells from apical papilla regulated by Zinc fingers and homeoboxes 2: An in vitro study.

Biochemical and biophysical research communications

Wan F,Gao L,Lu Y,Ma H,Wang H,Liang X,Wang Y,Ma C

Fri Jan 15 00:00:00 UTC 2016

Proliferation and osteo/odontogenic differentiation of stem cells from apical papilla regulated by Zinc fingers and homeoboxes 2: An in vitro study.

Biochemical and biophysical research communications

Wan F,Gao L,Lu Y,Ma H,Wang H,Liang X,Wang Y,Ma C

Fri Jan 15 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: AFP regulator 1; alpha fetoprotein regulation 1, adult; Alpha-fetoprotein regulator 1; Regulator of AFP; transcription factor ZHX2; Zinc finger and homeodomain protein 2; Zinc fingers and homeoboxes protein 2; zinc-fingers and homeoboxes 2

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Gene Aliases: Afr-1; AFR1; KIAA0854; mKIAA0854; RAF; ZHX2

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UniProt ID: (Human) Q9Y6X8, (Rat) Q80VX4, (Mouse) Q8C0C0

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Entrez Gene ID: (Human) 22882, (Rat) 314988, (Mouse) 387609

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Function(s)

DNA binding transcription factor activity, sequence-specific DNA binding transcription corepressor activity protein binding identical protein binding protein homodimerization activity metal ion binding protein heterodimerization activity

Process(es)

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated regulation of transcription from RNA polymerase II promoter mRNA catabolic process somatic stem cell population maintenance negative regulation of neuron differentiation negative regulation of transcription, DNA-templated regulation of transcription, DNA-templated negative regulation of nucleic acid-templated transcription

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ZHX2 Polyclonal Antibody

ZHX2 Antibody (20136-1-AP) in ICC/IF

Product Details

20136-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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