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ZIC-2 Antibody (600-401-848) in WB
Western blot using Rocklands Affinity Purified anti-ZIC2 antibody shows detection of a band ~55 kDa (arrowhead) corresponding to ZIC2 in lysates from (lane 1) mouse brain and (lane 2) rat brain. Approximately 20 µg of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-ZIC2 antibody. Signal was detected using standard techniques. The upper band in the mouse brain lysate represents Zic2.
Product Details
600-401-848
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Chimpanzee,
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 180-210 of Mouse ZIC-2.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against mouse ZIC2 protein. A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee, mouse, rat and dog based on 100% homology for the immunogen sequence. Expect cross reactivity with ZIC2 from chicken, zebrafish and frog as the core 16 amino acid residues within the immunogen sequence show very high degrees of homology with ZIC2 from these sources. Cross reactivity with ZIC2 homologues from other sources has not been determined.
Target Information
ZIC2 (zinc finger protein of the cerebellum 2) is a transcriptional repressor and may regulate tissue specific expression of dopamine receptor D1. In mouse, knockout of Zic1 leads to aplasia of cerebellum and skeletal abnormalities. Mutation in Zic2 and Zic3 in mouse and/or human cause holoprosencephaly and heterotaxis, respectively. Holoprosencephaly is the most common structural anomaly of the human brain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cerebellum zinc finger protein 2; GENA 29; kumba; odd-paired homolog; Zic family member 2 (odd-paired homolog, Drosophila); Zinc finger protein of the cerebellum 2; Zinc finger protein ZIC 2
Gene Aliases: HPE5; Ku; ZIC2
UniProt ID: (Human) O95409
Entrez Gene ID: (Dog) 485533, (Human) 7546, (Rat) 361096, (Mouse) 22772
DNA binding
transcription factor activity, sequence-specific DNA binding
chromatin DNA binding
metal ion binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
nucleic acid binding
protein binding
neural tube closure
transcription from RNA polymerase II promoter
nervous system development
central nervous system development
visual perception
cell differentiation
retinal ganglion cell axon guidance
cilium organization
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
developmental pigmentation
positive regulation of sequence-specific DNA binding transcription factor activity
positive regulation of nodal signaling pathway involved in determination of lateral mesoderm left/right asymmetry
transcription, DNA-templated
regulation of transcription, DNA-templated
multicellular organism development
brain development
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