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Product Details
PA5-62351
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human ZNF281. Recombinant protein control fragment (Product #RP-101420).
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2650044
Product Specific Information
Immunogen sequence: SSPLHNHTLF PEKQIYTTSP LECGFGQSVT SVLPSSLPKP PFGMLFGSQP GLYLSALDAT HQQLTPSQEL DDLIDSQKNL ETSSAFQSSS QKLTSQKEQ
Highest antigen sequence identity to the following orthologs: Mouse - 100%, Rat - 100%.
Target Information
ZFP281, also known as ZBP-99, is a member of a conserved family of transcription factors that interact with GC-rich promoters. It contains four Kruppel-like zinc fingers and is highly homologous to ZBP-89, another regulator of ornithine decarboxylase gene expression. ZFP281 also interacts with many other other transcription factors, such as Sp1 to repress vimentin gene transcription. ZFP281 has also been shown to control embryonic stem cell pluripotency by direct regulating specific target genes. ZFP281 phyically interacts with the transcription factos POU5F1, Sox2, and NANOG. Gene expression microarray anlysis indicated that some ZFP281 target genes were activated, while others were repressed upon knockdown of ZFP281 expression, showing that ZFP281 plays bifuctinal roles in regulating gene expression in stem cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GC-box-binding zinc finger protein 1; Transcription factor ZBP-99; Zinc finger DNA-binding protein 99; Zinc finger protein 281; ZNP-99 transcription factor
Gene Aliases: GZP1; ZBP-99; ZBP99; ZNF281; ZNP-99
UniProt ID: (Human) Q9Y2X9
Entrez Gene ID: (Human) 23528
RNA polymerase II core promoter proximal region sequence-specific DNA binding
core promoter binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
transcription corepressor activity
protein binding
sequence-specific DNA binding
transcription regulatory region DNA binding
metal ion binding
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