Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
ZNF306 Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 2
ZNF306 Antibody (PA5-102903) in ICC/IF
Immunocytochemistry analysis of ZNF306 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with ZNF306 polyclonal antibody (Product # PA5-102903) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-102903
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ZKSCAN3(Accession Q9BRR0), corresponding to amino acid residues G212-D262.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2852284
Product Specific Information
Antibody detects endogenous levels of total ZKSCAN3.
Target Information
ZNF306 is a protein coding gene. Transcriptional factor that binds to the consensus sequence 5'-[GT][AG][AGT]GGGG-3' and acts as a repressor of autophagy. Specifically represses expression of genes involved in autophagy and lysosome biogenesis/function such as MAP1LC3B, ULK1 or WIPI2. Associates with chromatin at the ITGB4 and VEGF promoters. Also acts as a transcription activator and promotes cancer cell progression and/or migration in various tumors and myelomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: SCAN-KRAB-zinc finger gene 1; SCAN-KRAB-zinc finger protein; Zf47; Zinc finger and SCAN domain-containing protein 13; Zinc finger protein 306; Zinc finger protein 307; Zinc finger protein 309; Zinc finger protein 47 homolog; Zinc finger protein with KRAB and SCAN domains 3; zinc finger protein zfp47
Gene Aliases: 2810435N07Rik; AA408594; AI132359; dJ874C20.1; dJ874C20.1.; mszf35; Skz1; ZF47; Zfp-47; ZFP306; Zfp307; ZFP47; ZKSCAN3; ZNF306; ZNF309; ZSCAN13; ZSCAN35
UniProt ID: (Human) Q9BRR0, (Mouse) Q91VW9
Entrez Gene ID: (Human) 80317, (Mouse) 72739
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
sequence-specific DNA binding
metal ion binding
nucleic acid binding
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
autophagy
lysosome organization
negative regulation of autophagy
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
negative regulation of cellular senescence
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support