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ZNF496 Antibody (BS-12815R) in IHC (P)
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ZNF496 Polyclonal Antibody, Unconjugated (bs-12815R) at 1:400 overnight at 4°C, DAB staining.
Product Details
BS-12815R
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide derived from human ZNF496, amino acids 1-100.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA
Contains
0.02% ProClin 300
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
ZNF496 contains a SCAN box, a KRAB-A domain, and four consensus C2H2-type zinc fingers preceded by a unique finger derivative, referred to herein as the C2HR motif. The C2HR motif functions to mediate protein-protein interaction with the cysteine-rich (C5HCH) domain of NSD1 in a Zn(II)-dependent fashion, and when tethered to RNA polymerase II promoters, represses transcription in an NSD1-dependent manner. ZNF496 contains a novel type of zinc finger motif that functions as a docking site for NSD1 and is more than just a degenerate evolutionary remnant of a C2H2 motif.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MGC15548; NSD1 (nuclear receptor binding SET-domain containing 1)-interacting zinc finger protein 1; NSD1-interacting zinc finger protein 1; Zinc finger protein 496; Zinc finger protein with KRAB and SCAN domains 17
Gene Aliases: NIZP1; Nizp11; ZFP496; ZKSCAN17; ZNF496; ZSCAN49
UniProt ID: (Human) Q96IT1, (Mouse) Q5SXI5
Entrez Gene ID: (Human) 84838, (Mouse) 268417
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