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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • ZO-1 Antibodies

          Proteintech

          ZO-1 Polyclonal Antibody

          1,023 References
          View all (42) ZO-1 antibodies

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          Datasheet
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          Datasheet
          Protocols
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          Cite ZO-1 Polyclonal Antibody

          • Antibody Testing Data (14)
          ZO-1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          ZO-1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 14

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          ZO-1 Antibody (21773-1-AP) in ICC/IF

          Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using ZO-1 antibody (Product # 21773-1-AP) at dilution of 1:2,000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          ZO-1 Antibody in Immunocytochemistry (ICC/IF)
          ZO-1 Antibody in Immunocytochemistry (ICC/IF)
          ZO-1 Antibody in Immunocytochemistry (ICC/IF)
          ZO-1 Antibody in Immunocytochemistry (ICC/IF)
          ZO-1 Antibody in Immunocytochemistry (ICC/IF)
          ZO-1 Antibody in Immunocytochemistry (ICC/IF)
          ZO-1 Antibody in Western Blot (WB)
          ZO-1 Antibody in Western Blot (WB)
          ZO-1 Antibody in Western Blot (WB)
          ZO-1 Antibody in Western Blot (WB)
          ZO-1 Antibody in Western Blot (WB)
          ZO-1 Antibody in Flow Cytometry (Flow)
          ZO-1 Antibody in Immunoprecipitation (IP)
          ZO-1 Antibody in Immunoprecipitation (IP)

          Product Details

          21773-1-AP

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:1,000
          View 588 publications 588 publications

          Immunohistochemistry (IHC)

          Assay-dependent
          View 104 publications 104 publications

          Immunocytochemistry (ICC/IF)

          1:20-1:200
          View 303 publications 303 publications

          Flow Cytometry (Flow)

          0.2 µg/1x10^6 cells
          View 3 publications 3 publications

          Immunoprecipitation (IP)

          0.5-4.0 µg
          -

          Miscellaneous PubMed (MISC)

          -
          View 5 publications 5 publications

          Not applicable (N/A)

          -
          View 20 publications 20 publications
          Product Specifications

          Species Reactivity

          Dog, Hamster, Human, Mouse, Rat

          Published species

          Bovine, Human, Mouse, Pig, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          ZO-1 Fusion Protein Ag16454 (1446-1748 aa encoded by BC111712)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.37 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice (domestic); Dry ice (international)

          Product Specific Information

          Immunogen sequence: SLHIHSKGA HGEGNSVSLD FQNSLVSKPD PPPSQNKPAT FRPPNREDTA QAAFYPQKSF PDKAPVNGTE QTQKTVTPAY NRFTPKPYTS SARPFERKFE SPKFNHNLLP SETAHKPDLS SKTPTSPKTL VKSHSLAQPP EFDSGVETFS IHAEKPKYQI NNISTVPKAI PVSPSAVEED EDEDGHTVVA TARGIFNSNG GVLSSIETGV SIIIPQGAIP EGVEQEIYFK VCRDNSILPP LDKEKGETLL SPLVMCGPHG LKFLKPVELR LPHCDPKTWQ NKCLPGDPNY LVGANCVSVL IDHF (1446-1748 aa encoded by BC111712)

          Target Information

          ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: DKFZp686M05161; MGC133289; OTTHUMP00000174520; Tight junction protein 1; tight junction protein 1 (zona occludens 1); Tight junction protein ZO-1; zona occludens 1; Zona occludens protein 1; zonula occludens 1 protein; Zonula occludens protein 1

          View more View less

          Gene Aliases: I79_005485; TJP1; ZO-1; ZO1

          View more View less

          UniProt ID: (Dog) O97758, (Human) Q07157, (Mouse) P39447

          View more View less

          Entrez Gene ID: (Dog) 403752, (Human) 7082, (Mouse) 21872, (Rat) 292994, (Chinese hamster) 100773110

          View more View less

          Function(s)
          protein binding calmodulin binding protein domain specific binding cadherin binding involved in cell-cell adhesion protein C-terminus binding
          Process(es)
          blastocyst formation sensory perception of sound establishment of endothelial intestinal barrier response to lipopolysaccharide response to drug negative regulation of vascular permeability response to ethanol response to magnetism cellular response to glucose stimulus bicellular tight junction assembly cellular response to calcium ion cell-cell junction assembly hippo signaling cell-cell adhesion cell-cell signaling involved in cell-cell junction organization
          It has to be done as per old AB suggested Products section.
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