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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • ZO-1 Antibodies

          Invitrogen

          ZO-1 Polyclonal Antibody

          Advanced Verification
          326 References
          View all (43) ZO-1 antibodies

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          Datasheet
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          Protocols
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          Cite ZO-1 Polyclonal Antibody

          • Antibody Testing Data (11)
          • Advanced Verification (2)
          ZO-1 Antibody in Immunocytochemistry (ICC/IF)
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          ZO-1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 13

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          ZO-1 Antibody (40-2200) in ICC/IF

          Immunofluorescence analysis of ZO-1 was performed using 90% confluent log phase Caco-2 IFN Gamma (50 ng/mL for 24hrs). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with ZO-1 Polyclonal Antibody (Product # 40-2200) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          ZO-1 Antibody in Immunocytochemistry (ICC/IF)
          ZO-1 Antibody in Immunocytochemistry (ICC/IF)
          ZO-1 Antibody in Immunocytochemistry (ICC/IF)
          ZO-1 Antibody in Immunocytochemistry (ICC/IF)
          ZO-1 Antibody in Immunocytochemistry (ICC/IF)
          ZO-1 Antibody in Immunocytochemistry (ICC/IF)
          ZO-1 Antibody in Immunocytochemistry (ICC/IF)
          ZO-1 Antibody in Immunohistochemistry (IHC)
          ZO-1 Antibody in Immunohistochemistry (IHC)
          ZO-1 Antibody in Immunohistochemistry (IHC)
          ZO-1 Antibody in Western Blot (WB)
          ZO-1 Antibody
          ZO-1 Antibody

          Product Details

          40-2200

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          Assay-dependent
          View 85 publications 85 publications

          Immunohistochemistry (IHC)

          -
          View 96 publications 96 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          -
          View 3 publications 3 publications

          Immunohistochemistry (PFA fixed) (IHC (PFA))

          -
          View 2 publications 2 publications

          Immunohistochemistry (Frozen) (IHC (F))

          Assay-dependent
          View 9 publications 9 publications

          Immunocytochemistry (ICC/IF)

          2.5-5 µg/mL
          View 122 publications 122 publications

          Flow Cytometry (Flow)

          -
          View 2 publications 2 publications

          ELISA (ELISA)

          -
          View 1 publication 1 publication

          Immunoprecipitation (IP)

          -
          View 2 publications 2 publications

          Miscellaneous PubMed (MISC)

          -
          View 4 publications 4 publications
          Product Specifications

          Species Reactivity

          Dog, Human, Mouse, Rat

          Published species

          Bovine, Chicken, Dog, Human, Mouse, Non-human primate, Pig, Rat, Xenopus, Zebrafish

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Synthetic peptide in the middle region of the ZO-1 protein.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.25 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.4

          Contains

          0.1% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2533456

          Product Specific Information

          40-2200 was used successfully in the immunofluorescence analysis of Zo-1 in MDCK cells.

          Target Information

          ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: DKFZp686M05161; MGC133289; OTTHUMP00000174520; Tight junction protein 1; tight junction protein 1 (zona occludens 1); Tight junction protein ZO-1; zona occludens 1; Zona occludens protein 1; zonula occludens 1 protein; Zonula occludens protein 1

          View more View less

          Gene Aliases: TJP1; ZO-1; ZO1

          View more View less

          UniProt ID: (Dog) O97758, (Human) Q07157, (Mouse) P39447

          View more View less

          Entrez Gene ID: (Dog) 403752, (Human) 7082, (Mouse) 21872, (Rat) 292994

          View more View less

          Function(s)
          protein binding calmodulin binding protein domain specific binding cadherin binding involved in cell-cell adhesion protein C-terminus binding
          Process(es)
          blastocyst formation sensory perception of sound establishment of endothelial intestinal barrier response to lipopolysaccharide response to drug negative regulation of vascular permeability response to ethanol response to magnetism cellular response to glucose stimulus bicellular tight junction assembly cellular response to calcium ion cell-cell junction assembly hippo signaling cell-cell adhesion cell-cell signaling involved in cell-cell junction organization
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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