Invitrogen

ZO-1 Monoclonal Antibody (R26.4C), eFluor™ 570, eBioscience™

ZO-1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 2

ZO-1 Antibody (41-9776-82) in ICC/IF

Immunocytochemistry of formaldehyde-fixed, permeabilized MDCK cells stained with 1 µg/mL of Rat IgG1 K Isotype Control eFluor® 570 (left) or 1 µg/mL of Anti-ZO-1 eFluor® 570 (right). Nuclei are stained with DAPI.

Product Image Gallery

ZO-1 Antibody — Fig. 6 Verification of the role of MMP3 in melanoma brain metastases. a Representative immunofluorescence images of MMP3 expression by M/Ms after PD166793 treatment. Normal: mice treated with 0.5% CMC without PD166793. PD: mice treated with PD166793 dissolved in 0.5% CMC. Scale bar: 20 mum. b Quantitative results for the proportion of MMP3 + M/Ms shown in ( a ); n = 13 images from the normal group and 9 images from the PD group, 3 mice per group. c Representative immunofluorescence image of ZO-1 expression in the brain after MMP3 inhibition. Scale bar: 20 mum. d Quantitative results for the proportion of ZO-1 + cells shown in ( c ); n = 12 images from the normal group and 7 images from the PD group, 3 mice per group. e Representative photograph of brain tissues after PD166793 treatment. f - g The number ( f ) and size ( g ) of melanoma brain metastases in the indicated groups; n = 9 mice per group. The data are presented as the mean +- SEM {TM}

Product Details

41-9776-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Immunocytochemistry (ICC/IF)

1 µg/mL

View 2 publications 2 publications

Product Specifications

Species Reactivity

Dog, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 570, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

R26.4C

Conjugate

eFluor™ 570 eFluor™ 570 eFluor™ 570

Excitation/Emission Max

556/569 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2573645

Product Specific Information

Description: The monoclonal antibody R26.4C recognizes the tight junction protein 1, ZO-1 (zona occludens) of rat, mouse, canine, and porcine. ZO-1 is a member of the MAGUK family of tight junction associated scaffolding proteins, each containing a PDZ, SH3 and guanylate kinase-like (GUK) domain. ZO-1 is an integral member of the tight junction complex which forms a barrier to paracellular movement of substances, separating apical and basolateral fluids in relation to the epithelial cell layer. ZO-1 forms complexes with family members ZO-2, ZO-3, as well as occludin, cingulin, claudin-1-8, actin, alpha-catenin, the Ras-effector molecule AF-6, ZONAB, Apg-2, and the junctional adhesion molecules (JAM)-A-C. ZO-1 is thought to anchor the actin cytoskeleton to the tight junction. ZO-1 and ZO-2 have been shown to regulate cell cycle and proliferation with recent evidence highlighting the down-regulation of ZO-1 and ZO-2 with cancer progression.

Applications Reported: This R26.4C antibody has been reported for use in immunohistochemical staining of frozen tissue sections, microscopy, and immunocytochemistry.

Applications Tested: This R26.4C antibody has been tested by immunocytochemistry of formaldehyde-fixed and permeablized cells and can be used at less than or equal to 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filter Recommendation: When using this eFluor® 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor® 555 or TRITC filter is acceptable.

Excitation: 555 nm; Emission: 570 nm

Target Information

ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Nanoarchitecture and molecular interactions of epithelial cell junction proteins revealed by super-resolution microscopy.

Annals of the New York Academy of Sciences

Naser AN,Guiler W,Lu Q,Chen YH

41-9776-82 was used in Immunocytochemistry to show that STORM reveals regional cellular junction nanoarchitecture previously uncharacterized, providing new insight into potential trans-compartmental modulation of protein functions.

Sat Oct 01 00:00:00 UTC 2022

Nanoarchitecture and molecular interactions of epithelial cell junction proteins revealed by super-resolution microscopy.

Annals of the New York Academy of Sciences

Naser AN,Guiler W,Lu Q,Chen YH

Sat Oct 01 00:00:00 UTC 2022

Soluble CD146, a cerebrospinal fluid marker for neuroinflammation, promotes blood-brain barrier dysfunction.

Theranostics

Wang D,Duan H,Feng J,Xiang J,Feng L,Liu D,Chen X,Jing L,Liu Z,Zhang D,Hao H,Yan X

Published figure using ZO-1 monoclonal antibody (Product # 41-9776-82) in Immunocytochemistry

Fri Apr 02 00:00:00 UTC 2021

Long-term characterization of activated microglia/macrophages facilitating the development of experimental brain metastasis through intravital microscopic imaging.

Journal of neuroinflammation

Qiao S,Qian Y,Xu G,Luo Q,Zhang Z

41-9776-82 was used in Immunohistochemistry-immunofluorescence to indicate that the activated M/Ms were essential in the development of melanoma brain metastasis, suggesting that M/Ms are a potential therapeutic target for tumor brain metastasis.

Mon Jan 07 00:00:00 UTC 2019

Bioinformatics

Protein Aliases: DKFZp686M05161; MGC133289; OTTHUMP00000174520; Tight junction protein 1; tight junction protein 1 (zona occludens 1); Tight junction protein ZO-1; zona occludens 1; Zona occludens protein 1; Zonula occludens protein 1

View more View less

Gene Aliases: TJP1; ZO-1; ZO1

View more View less

UniProt ID: (Dog) O97758, (Mouse) P39447

View more View less

Entrez Gene ID: (Dog) 403752, (Mouse) 21872, (Rat) 292994

View more View less

Function(s)

protein binding calmodulin binding protein C-terminus binding protein domain specific binding

Process(es)

blastocyst formation sensory perception of sound establishment of endothelial intestinal barrier response to lipopolysaccharide response to drug negative regulation of vascular permeability response to ethanol response to magnetism cellular response to glucose stimulus bicellular tight junction assembly cellular response to calcium ion

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

ZO-1 Monoclonal Antibody (R26.4C), eFluor™ 570, eBioscience™

ZO-1 Antibody (41-9776-82) in ICC/IF

Product Details

41-9776-82

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!