Invitrogen

ZO-1 Monoclonal Antibody (ZO1-1A12), Alexa Fluor™ 647

3 Published Figures

10 References

View all (42) ZO-1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (3)

ZO-1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 4

ZO-1 Antibody (MA3-39100-A647) in ICC/IF

Immunofluorescent analysis of ZO-1 (red) in Caco-2 cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a ZO-1 Monoclonal Antibody, AlexaFluor 647 conjugate (Product # MA3-39100-A647) at a dilution of 5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Imag... View More

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Product Details

MA3-39100-A647

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1-10 µg/mL

View 5 publications 5 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

ZO1-1A12

Immunogen

Human recombinant ZO-1 fusion protein encompassing amino acids 334-634

View immunogen

Conjugate

Alexa Fluor™ 647 Alexa Fluor™ 647 Alexa Fluor™ 647

Excitation/Emission Max

650/671 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2663167

Target Information

ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Prime editing-mediated correction of the CFTR W1282X mutation in iPSCs and derived airway epithelial cells.

PloS one

Li C,Liu Z,Anderson J,Liu Z,Tang L,Li Y,Peng N,Chen J,Liu X,Fu L,Townes TM,Rowe SM,Bedwell DM,Guimbellot J,Zhao R

MA3-39100-A647 was used in Immunocytochemistry to demonstrate that prime editing may potentially be applied to correct CFTR mutations such as W1282X. Copyright:.

Fri Dec 01 00:00:00 UTC 2023

Signal transduction and targeted therapy

Shi X,Wang Z,Ren W,Chen L,Xu C,Li M,Fan S,Xu Y,Chen M,Zheng F,Zhang W,Zhou X,Zhang Y,Qiu S,Wu L,Zhou P,Lv X,Cui T,Qiao Y,Zhao H,Guo W,Chen W,Li S,Zhong W,Lin J,Yang S

MA3-39100-A647 was used in Immunohistochemistry-immunofluorescence to develop an LRP1-binding peptide, IETP2, and covalently conjugated a series of model small-molecule compounds to it, including potential drugs and imaging agents.

Fri Jun 10 00:00:00 UTC 2022

Nephrin expression in human epidermal keratinocytes and its implication in poor wound closure.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Kim JY,Lee EJ,Seo J,Lee Y,Ahn Y,Park S,Bae YJ,Lee J,Lim BJ,Kim D,Cho JW,Oh SH

MA3-39100-A647 was used in Immunohistochemistry-immunofluorescence to suggest that nephrin may have a variety of physiological roles in human skin.

Fri Jul 01 00:00:00 UTC 2022

Culture and Imaging of Human Nasal Epithelial Organoids.

Journal of visualized experiments : JoVE

Liu Z,Anderson JD,Natt J,Guimbellot JS

MA3-39100-A647 was used in Immunocytochemistry to understand baseline Cystic Fibrosis Transmembrane conductance Regulator (CFTR) activity and restoration from small molecule compounds.

Fri Dec 17 00:00:00 UTC 2021

Human Nasal Epithelial Organoids for Therapeutic Development in Cystic Fibrosis.

Genes

Liu Z,Anderson JD,Deng L,Mackay S,Bailey J,Kersh L,Rowe SM,Guimbellot JS

MA3-39100-A647 was used in Immunocytochemistry to assess correction of the mutant gene in CF or other diseases, recapitulating the phenotypic and genotypic diversity of the population.

Fri May 29 00:00:00 UTC 2020

Human Nasal Epithelial Organoids for Therapeutic Development in Cystic Fibrosis.

Genes

Liu Z,Anderson JD,Deng L,Mackay S,Bailey J,Kersh L,Rowe SM,Guimbellot JS

MA3-39100-A647 was used in Immunocytochemistry to assess correction of the mutant gene in CF or other diseases, recapitulating the phenotypic and genotypic diversity of the population.

Fri May 29 00:00:00 UTC 2020

Comparative Analysis of Cell-Cell Contact Abundance in Ovarian Carcinoma Cells Cultured in Two- and Three-Dimensional In Vitro Models.

Biology

Kutova OM,Sencha LM,Pospelov AD,Dobrynina OE,Brilkina AA,Cherkasova EI,Balalaeva IV

MA3-39100-A647 was used in Flow cytometry/Cell sorting to compare the abundance of contact proteins in SKOV-3 and SKOV-3.ip human ovarian adenocarcinoma cell lines, cultivated in monolayers, tumor spheroids and collagen hydrogels.

Fri Dec 04 00:00:00 UTC 2020

Comparative Analysis of Cell-Cell Contact Abundance in Ovarian Carcinoma Cells Cultured in Two- and Three-Dimensional In Vitro Models.

Biology

Kutova OM,Sencha LM,Pospelov AD,Dobrynina OE,Brilkina AA,Cherkasova EI,Balalaeva IV

Fri Dec 04 00:00:00 UTC 2020

Mitochondrial phosphatase PGAM5 modulates cellular senescence by regulating mitochondrial dynamics.

Nature communications

Yu B,Ma J,Li J,Wang D,Wang Z,Wang S

MA3-39100-A647 was used in Immunohistochemistry (PFA fixed) to demonstrate that deletion of mitochondrial phosphatase PGAM5 leads to accelerated retinal pigment epithelial (RPE) senescence in vitro and in vivo.

Thu May 21 00:00:00 UTC 2020

Mitochondrial phosphatase PGAM5 modulates cellular senescence by regulating mitochondrial dynamics.

Nature communications

Yu B,Ma J,Li J,Wang D,Wang Z,Wang S

Thu May 21 00:00:00 UTC 2020

Mitochondrial phosphatase PGAM5 modulates cellular senescence by regulating mitochondrial dynamics.

Nature communications

Yu B,Ma J,Li J,Wang D,Wang Z,Wang S

Thu May 21 00:00:00 UTC 2020

Inhibition of MicroRNA-155 Supports Endothelial Tight Junction Integrity Following Oxygen-Glucose Deprivation.

Journal of the American Heart Association

Pena-Philippides JC,Gardiner AS,Caballero-Garrido E,Pan R,Zhu Y,Roitbak T

MA3-39100-A647 was used in Immunocytochemistry-immunoflourescence to investigate the influence of microRNA-155 on the barrier characteristics of human primary brain microvascular endothelial cells.

Tue Jun 26 00:00:00 UTC 2018

Bioinformatics

Protein Aliases: DKFZp686M05161; MGC133289; OTTHUMP00000174520; Tight junction protein 1; tight junction protein 1 (zona occludens 1); Tight junction protein ZO-1; zona occludens 1; Zona occludens protein 1; zonula occludens 1 protein; Zonula occludens protein 1

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Gene Aliases: TJP1; ZO-1; ZO1

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UniProt ID: (Dog) O97758, (Human) Q07157

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Entrez Gene ID: (Dog) 403752, (Human) 7082

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Function(s)

protein binding calmodulin binding protein domain specific binding cadherin binding involved in cell-cell adhesion

Process(es)

bicellular tight junction assembly cellular response to calcium ion blastocyst formation cell-cell junction assembly sensory perception of sound hippo signaling establishment of endothelial intestinal barrier cell-cell adhesion cell-cell signaling involved in cell-cell junction organization

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ZO-1 Monoclonal Antibody (ZO1-1A12), Alexa Fluor™ 647

ZO-1 Antibody (MA3-39100-A647) in ICC/IF

Product Details

MA3-39100-A647

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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