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Product Details
MUB0110P
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chicken,
Human,
Mouse,
Pig,
Rabbit,
Rat,
Zebrafish
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
4C2
Immunogen
actin beta-cytoplasmic
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A/G
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
4C2 is a mouse monoclonal Ig1 antibody highly specific for beta-cytoplasmic actin.
The epitope recognized by 4C2 is highly conserved. The antibody therefore cross-reacts with many species including protochordates, lower craniates and mammals.
The antibody may be stored at 4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
Target Information
Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: actin, beta; Actin, cytoplasmic 1; B-ACTZF; beta cytoskeletal actin; Beta-actin; Beta-actin-1; cytoplasmic 1; cytoplasmic beta-actin; gamma non-muscle actin; PS1TP5-binding protein 1
Gene Aliases: ACT-5; ACTB; actba; actin; Actx; B-ACTZF; Bact; bactin1; bactzf; beta-actin; BRWS1; E430023M04Rik; PS1TP5BP1; RCJMB04_4h19; wu:fd18f05
UniProt ID: (Rabbit) P29751, (Human) P60709, (Pig) Q6QAQ1, (Chicken) P60706, (Rat) P60711, (Mouse) P60710, (Zebrafish) Q7ZVI7
Entrez Gene ID: (Rabbit) 100009272, (Human) 60, (Pig) 414396, (Chicken) 396526, (Rat) 81822, (Mouse) 11461, (Zebrafish) 57934
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
structural constituent of cytoskeleton
protein binding
ATP binding
kinesin binding
Tat protein binding
nucleosomal DNA binding
identical protein binding
nitric-oxide synthase binding
protein kinase binding
nucleotide binding
retina homeostasis
movement of cell or subcellular component
cytoskeleton organization
substantia nigra development
cell junction assembly
Fc-gamma receptor signaling pathway involved in phagocytosis
ATP-dependent chromatin remodeling
positive regulation of gene expression, epigenetic
vascular endothelial growth factor receptor signaling pathway
ephrin receptor signaling pathway
platelet aggregation
axonogenesis
retina development in camera-type eye
cellular response to electrical stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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