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Product Details
MUB0111P
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chicken,
Human,
Mouse,
Pig,
Rabbit,
Rat,
Zebrafish
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
2A3
Immunogen
actin gamma-cytoplasmic
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A/G
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
2A3 is a mouse monoclonal IgG2b antibody highly specific for gamma-cytoplasmic actin (gamma-actin 1; encoded by ACTG1). It does not cross-react with the gamma-actin isoform expressed in the enteric smooth muscle tissues (gamma-actin 2; encoded by ACTG2).
The epitope recognized by α-SM1 is highly conserved. The antibody therefore cross-reacts with many species including protochordates, lower craniates and mammals.
The antibody may be stored at 4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
Target Information
Actins are highly conserved proteins that are involved in various types of cell motility and in maintenance of the cytoskeleton. Three main groups of actin isoforms have been identified in vertebrate animals: alpha, beta, and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. Actin gamma 1, encoded by this gene, is a cytoplasmic actin found in all cell types. Mutations in this gene are associated with DFNA20/26, a subtype of autosomal dominant non-syndromic sensorineural progressive hearing loss and also with Baraitser-Winter syndrome. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: actin gamma; Actin, cytoplasmic 2; Actin, cytoplasmic type 5; actin, cytoplasmic, type 5; actin, gamma 1 propeptide; actin, gamma, cytoplasmic 1; beta-actin FE-3; cytoplasmic 2; cytoplasmic ACTG1; cytoplasmic type 5; cytoskeletal gamma-actin; cytoskeleton actin; deafness, autosomal dominant 20; deafness, autosomal dominant 26; epididymis luminal protein 176; gamma 1 propeptide; gamma actin; Gamma-actin; type 5
Gene Aliases: ACT; ACTG; ACTG1; actin; Actl; AL023024; BRWS2; cytoplasmic; DFNA20; DFNA26; E51; HEL-176; RCJMB04_1h13
UniProt ID: (Chicken) P53478, (Human) P63261, (Mouse) P63260, (Rat) P63259
Entrez Gene ID: (Pig) 397653, (Chicken) 415296, (Human) 71, (Mouse) 11465, (Rat) 287876
sarcomere organization
retina homeostasis
movement of cell or subcellular component
cell junction assembly
Fc-gamma receptor signaling pathway involved in phagocytosis
vascular endothelial growth factor receptor signaling pathway
ephrin receptor signaling pathway
platelet aggregation
response to calcium ion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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