Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
alpha-1 Antitrypsin Monoclonal Antibody (2B12)
FIGURE: 1 / 6
alpha-1 Antitrypsin Antibody (MA5-15521) in WB
Western blot was performed using Anti-alpha-1 Antitrypsin Monoclonal Antibody (2B12)(Product # MA5-15521) and a ~55kDa band corresponding to alpha-1 Antitrypsin was observed across cell lines. Whole Cell Extract-TEB (30 µg lysate) of Hep G2 (Lane 1) and HT-29 (Lane 2) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:... View More
Product Details
MA5-15521
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
2B12
Immunogen
Purified recombinant fragment of human AAT expressed in E. Coli.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.03% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10985373
Product Specific Information
MA5-15521 targets AAT in indirect ELISA, WB applications and shows reactivity with Human and mouse samples.
The MA5-15521 immunogen is purified recombinant fragment of human AAT expressed in E. Coli.
MA5-15521 detects AAT which has a predicted molecular weight of approximately 47kDa.
Target Information
Alpha-1-AT is synthesized in the liver and it acts as an inhibitor of proteases such as trypsin, elastase, chymotrypsin, collagenase, leucocytic proteases, plasmin, and thrombin, which may be released during inflammatory reactions in the lung. In the absence of alpha-1-AT, these enzymes are not inhibited and they may digest pulmonary parenchyma. Alpha-1-AT deficiency is associated with chronic obstructive lung disease (emphysema) and less frequently with hepatic cirrhosis in infants and respiratory distress of the newborn. Increase in alpha-1-AT occurs as an acute phase response to tissue necrosis and inflammation. Serum level of alpha-1-AT is elevated in rheumatoid arthritis, bacterial infections, vasculitis, and carcinomatosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: a-1 AT; a1-antitrypsin; AAT; alpha 1 antitrypsin; alpha-1 antitrypsin; Alpha-1 protease inhibitor; Alpha-1 protease inhibitor 1; Alpha-1-antiproteinase; Alpha-1-antitrypsin; Alpha-1-antitrypsin 1-1; alpha-1-antitrypsin 2; alpha-1-antitrypsin null; Digestive Zymogen; MGC23330; MGC9222; protease inhibitor 1 (anti-elastase), alpha-1-antitrypsin; Protease Serine 1; serine (or cysteine) proteinase inhibitor, clade A, member 1; serine (or cysteine) proteinase inhibitor, clade A, member 1a; Serine Protease 1; Serine protease inhibitor 1-1; Serine protease inhibitor A1a; Serpin A1; Serpin A1a; serpin peptidase inhibitor clade A member 1; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1; TRP1; TRY1
Gene Aliases: A1A; A1AT; AAT; Aat-2; Aat2; alpha1AT; Dom1; PI; PI1; PRO0684; PRO2209; PRO2275; SERPINA1; Serpina1a; Spi1-1; Spi1-3
UniProt ID: (Human) P01009, (Mouse) P07758
Entrez Gene ID: (Human) 5265, (Mouse) 20700
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support