Alomone Labs, LTD

alpha 1A-Adrenergic Receptor (extracellular) Polyclonal Antibody

View all (11) alpha-1a Adrenergic Receptor antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

AAR-015-200UL

Applications
Tested Dilution
Publications

Western Blot (WB)

1:200
-

Immunohistochemistry (IHC)

1:100
-

Flow Cytometry (Flow)

2.5 µg
-
Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

EDETI*SQINEEPG(C), corresponding to amino acid residues 171-183 of human alpha 1A-adrenoceptor with replacement of cysteine 176 (C176) with serine (*S), 2nd extracellular loop
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.7 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Information

Adrenergic receptors (ARs) are members of the 7-transmembrane domain G-protein-coupled receptor superfamily that bind the endogenous catecholamines epinephrine and norepinephrine. Pharmacological, structural, and molecular cloning data indicate significant heterogeneity within this receptor family. Nine receptor subtypes have been identified thus far including three alpha-1 AR subtypes (1A/D, 1B, and 1C), three alpha-2 ARs (2A, 2B, and 2C), and three beta AR subtypes (1, 2, and 3). ARs participate in either the onset or maintenance of several disease states including hypertension, cardiac dysfunction (congestive heart failure, ischemia, arrhythmias), diabetes, glaucoma, depression, and impotence. A1AR subtypes are found in numerous tissues and have been shown to be involved in the regulation of blood pressure due to changes in vascular tone and cardiac output. Effects on uterine contraction, hepatic glucose metabolism, heat shock protein 70 (HSP70), proto-oncogene expression, and mitogenesis have been linked to A1AR activation. Norepinephrine can increase nitric oxide synthase (NOS) levels in the hypothalamus by activating alpha-1 AR which may indirectly suppress the release of luteinizing hormone (LH), the androgens, estrogen, and progesterone.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ADA1; adrenergic alpha 1c receptor; adrenergic receptor alpha 1c subtype; adrenergic receptor, alpha 1a; adrenergic receptor, alpha 1c; adrenergic, alpha-1A-, receptor; alpha 1A-adrenoceptor; alpha 1A-adrenoreceptor; alpha 1C-adrenergic receptor; Alpha-1A adrenergic receptor; Alpha-1A adrenoceptor; Alpha-1A adrenoreceptor; Alpha-1C adrenergic receptor; Alpha-adrenergic receptor 1c; Alpha1C-AR; G protein coupled receptor

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Gene Aliases: ADRA1A; ADRA1C; ADRA1L1; ALPHA1AAR

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UniProt ID: (Human) P35348, (Rat) P43140

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Entrez Gene ID: (Human) 148, (Rat) 29412

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Function(s)
alpha1-adrenergic receptor activity protein heterodimerization activity
Process(es)
negative regulation of heart rate involved in baroreceptor response to increased systemic arterial blood pressure norepinephrine-epinephrine vasoconstriction involved in regulation of systemic arterial blood pressure positive regulation of heart rate by epinephrine-norepinephrine positive regulation of the force of heart contraction by epinephrine-norepinephrine positive regulation of systemic arterial blood pressure phospholipase C-activating G-protein coupled receptor signaling pathway activation of phospholipase C activity positive regulation of cytosolic calcium ion concentration adult heart development aging response to hormone positive regulation of heart rate cell growth positive regulation of synaptic transmission, GABAergic negative regulation of Rho protein signal transduction organ growth response to drug positive regulation of MAPK cascade positive regulation of action potential positive regulation of vasoconstriction positive regulation of smooth muscle contraction micturition calcium ion transport into cytosol positive regulation of cardiac muscle contraction positive regulation of ERK1 and ERK2 cascade adrenergic receptor signaling pathway positive regulation of protein kinase C signaling pilomotor reflex apoptotic process smooth muscle contraction signal transduction G-protein coupled receptor signaling pathway cell-cell signaling negative regulation of cell proliferation intracellular signal transduction adenylate cyclase-activating adrenergic receptor signaling pathway
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