Invitrogen

alpha-2a Adrenergic Receptor Polyclonal Antibody

alpha-2a Adrenergic Receptor Antibody in Western Blot (WB)

FIGURE: 1 / 9

alpha-2a Adrenergic Receptor Antibody (PA1-048) in WB

Western blot analysis was performed on whole cell extracts (30 µg lysate) of PC-3 (Lane 1), HEK-293 (Lane 2), T47D (Lane 3) and HEL 92.1.7 (lane 4). The blots were probed with Anti-alpha-2a Adrenergic Receptor Rabbit Polyclonal Antibody (Product # PA1-048, 1:250-1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (Product # G-21234, 1:5000 di... View More

alpha-2a Adrenergic Receptor Antibody in Immunohistochemistry (Frozen) (IHC (F))

alpha-2a Adrenergic Receptor Antibody in Immunohistochemistry (IHC)

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Product Details

PA1-048

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000

Immunohistochemistry (Frozen) (IHC (F))

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corressponding to residues R(218) I Y Q I A K R R T R V P P S R R G(235) of the 3rd intracellular loop of human A2AAR.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.6 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2225243

Product Specific Information

PA1-048 detects alpha-2A adrenergic receptor (A2AAR) from human, rat and mouse tissues.

PA1-048 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects an ~45 kDa protein representing A2AAR from mouse kidney membrane preparations.

The PA1-048 immunogen is a synthetic peptide corressponding to residues R(218) I Y Q I A K R R T R V P P S R R G(235) of the 3rd intracellular loop of human A2AAR. This sequence is completely conserved between human, mouse, rat, and porcine A2AAR.

Target Information

Alpha-2-adrenergic receptors (ADRA2A) are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C. ADRA2A mediate the catecholamine-induced inhibition of adenylate cyclase through the action of G protein. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Singular versus combinatory glucose-sensitive signal control of metabolic sensor protein profiles in hypothalamic astrocyte cultures from each sex.

Translational neuroscience

Alhamyani A,Napit PR,Bheemanapally K,Sylvester PW,Briski KP

PA1-048 was used in Immunohistochemistry (Paraffin), Western Blot to show that DEX and NE regulation of GLUT2 is sex-monomorphic, but both stimuli impose divergent sex-specific effects on AMPK and pAMPK.

Sat Jan 01 00:00:00 UTC 2022

Capillary Western Immunoassay Optimization of Estrogen Related Factors in Human Subcutaneous Adipose Tissue.

Methods and protocols

Hill JL,McIver KB,Katzer K,Foster MT

PA1-048 was used in Simple Western - Size to evaluate estrogen related factors, such as, but not limited to, estrogen receptors and enzymes involved in the successive conversions of cholesterol and androgens to estrogens in human subcutaneous adipose.

Fri Apr 15 00:00:00 UTC 2022

Carotid smooth muscle contractility changes after severe burn.

Scientific reports

DeSpain K,Rosenfeld CR,Huebinger R,Wang X,Jay JW,Radhakrishnan RS,Wolf SE,Song J

PA1-048 was used in Immunohistochemistry to investigate the changes in carotid artery contractility and relaxation following a severe burn.

Fri Sep 10 00:00:00 UTC 2021

Carotid smooth muscle contractility changes after severe burn.

Scientific reports

DeSpain K,Rosenfeld CR,Huebinger R,Wang X,Jay JW,Radhakrishnan RS,Wolf SE,Song J

PA1-048 was used in Immunohistochemistry to investigate the changes in carotid artery contractility and relaxation following a severe burn.

Fri Sep 10 00:00:00 UTC 2021

Presynaptic Release-Regulating Alpha2 Autoreceptors: Potential Molecular Target for Ellagic Acid Nutraceutical Properties.

Antioxidants (Basel, Switzerland)

Romeo I,Vallarino G,Turrini F,Roggeri A,Olivero G,Boggia R,Alcaro S,Costa G,Pittaluga A

PA1-048 was used in Western Blotting to examine ellagic acid targets such as alpha2 auto receptors.

Thu Nov 04 00:00:00 UTC 2021

Circuit-specific hippocampal ΔFosB underlies resilience to stress-induced social avoidance.

Nature communications

Eagle AL,Manning CE,Williams ES,Bastle RM,Gajewski PA,Garrison A,Wirtz AJ,Akguen S,Brandel-Ankrapp K,Endege W,Boyce FM,Ohnishi YN,Mazei-Robison M,Maze I,Neve RL,Robison AJ

PA1-048 was used in Immunohistochemistry to study the molecular, cellular, and circuit-level mechanisms for depression- and anxiety-like behaviour in response to stress and the role of ΔFosB in behaviourally diverse responses to stress.

Tue Sep 08 00:00:00 UTC 2020

Circuit-specific hippocampal ΔFosB underlies resilience to stress-induced social avoidance.

Nature communications

Eagle AL,Manning CE,Williams ES,Bastle RM,Gajewski PA,Garrison A,Wirtz AJ,Akguen S,Brandel-Ankrapp K,Endege W,Boyce FM,Ohnishi YN,Mazei-Robison M,Maze I,Neve RL,Robison AJ

Tue Sep 08 00:00:00 UTC 2020

Zerumbone Modulates α_2A-Adrenergic, TRPV1, and NMDA NR2B Receptors Plasticity in CCI-Induced Neuropathic Pain In Vivo and LPS-Induced SH-SY5Y Neuroblastoma In Vitro Models.

Frontiers in pharmacology

Chia JSM,Izham NAM,Farouk AAO,Sulaiman MR,Mustafa S,Hutchinson MR,Perimal EK

Published figure using alpha-2a Adrenergic Receptor polyclonal antibody (Product # PA1-048) in Western Blot

Mon Sep 28 00:00:00 UTC 2020

Norepinephrine Regulation of Adrenergic Receptor Expression, 5' AMP-Activated Protein Kinase Activity, and Glycogen Metabolism and Mass in Male Versus Female Hypothalamic Primary Astrocyte Cultures.

ASN neuro

Ibrahim MMH,Bheemanapally K,Sylvester PW,Briski KP

PA1-048 was used in Western Blot to investigate the premise that NE imposes sex-specific direct control of AMPK activity and glycogen mass and metabolism in these glia, using a rat hypothalamic astrocyte primary culture model.

Wed Sep 15 00:00:00 UTC 2021

Brimonidine Protects Auditory Hair Cells from in vitro-Induced Toxicity of Gentamicin.

Audiology & neuro-otology

Cortada M,Levano S,Bodmer D

PA1-048 was used in Western Blotting to demonstrate that brimonidine, acting directly via the alpha-2 adrenergic receptor, protects hair cells from gentamicin-induced toxicity.

Fri Jul 27 00:00:00 UTC 2018

Morphological correlates of sex differences in acoustic startle response and prepulse inhibition through projections from locus coeruleus to cochlear root neurons.

Brain structure & function

Hormigo S,Gómez-Nieto R,Sancho C,Herrero-Turrión J,Carro J,López DE,Horta-Júnior JACE

PA1-048 was used in Immunohistochemistry (Paraffin), Western Blot to support the participation of LC in ASR and PPI, and contributes toward a better understanding of sex-related differences observed in somatosensory gating paradigms.

Wed Nov 01 00:00:00 UTC 2017

Single-cell analysis of long non-coding RNAs in the developing human neocortex.

Genome biology

Liu SJ,Nowakowski TJ,Pollen AA,Lui JH,Horlbeck MA,Attenello FJ,He D,Weissman JS,Kriegstein AR,Diaz AA,Lim DA

PA1-048 was used in immunohistochemistry - frozen section to study the developing human neocortex for single-cell analysis of long non-coding RNAs

Thu Apr 14 00:00:00 UTC 2016

Low-coverage single-cell mRNA sequencing reveals cellular heterogeneity and activated signaling pathways in developing cerebral cortex.

Nature biotechnology

Pollen AA,Nowakowski TJ,Shuga J,Wang X,Leyrat AA,Lui JH,Li N,Szpankowski L,Fowler B,Chen P,Ramalingam N,Sun G,Thu M,Norris M,Lebofsky R,Toppani D,Kemp DW,Wong M,Clerkson B,Jones BN,Wu S,Knutsson L,Alvarado B,Wang J,Weaver LS,May AP,Jones RC,Unger MA,Kriegstein AR,West JA

PA1-048 was used in immunohistochemistry - frozen section to analyze developing cerebral cortex and low-coverage single-cell mRNA sequencing that reveals activated signaling pathways and cellular heterogeneity

Wed Oct 01 00:00:00 UTC 2014

Immunohistochemical localization of α2-adrenergic receptors in the neonatal rat cochlea and the vestibular labyrinth.

Journal of molecular neuroscience : MN

Cai J,Li J,Mao Y,Bai X,Xu L,Wang H

PA1-048 was used in immunohistochemistry to study the immunohistochemical expression of the three alpha2-adrenergic receptor sub-types in the cochlea and vestibular labyrinth of neonatal rats

Fri Nov 01 00:00:00 UTC 2013

Stimulation of α(2A)-adrenoceptors promotes the maturation of dendritic spines in cultured neurons of the medial prefrontal cortex.

Molecular and cellular neurosciences

Ren WW,Liu Y,Li BM

PA1-048 was used in immunocytochemistry to study the role of the alpha2A-adrenoceptors in the maturation of dendritic spines in the medial prefrontal cortex

Wed Feb 01 00:00:00 UTC 2012

Alpha2-adrenergic regulation of NO production alters postoperative intestinal smooth muscle dysfunction in rodents.

American journal of physiology. Gastrointestinal and liver physiology

Kreiss C,Toegel S,Bauer AJ

PA1-048 was used in immunohistochemistry to investigate the possible effects of alpha 2-adrenergic blockade with yohimbine on postsurgical intestinal smooth muscle dysfunction and NO production

Wed Sep 01 00:00:00 UTC 2004

Altered prejunctional modulation of intestinal cholinergic and noradrenergic pathways by alpha2-adrenoceptors in the presence of experimental colitis.

British journal of pharmacology

Blandizzi C,Fornai M,Colucci R,Baschiera F,Barbara G,De Giorgio R,De Ponti F,Breschi MC,Del Tacca M

PA1-048 was used in western blot to investigate the role of alpha2-adrenoceptors in intestinal inflammation

Thu May 01 00:00:00 UTC 2003

Bioinformatics

Protein Aliases: adrenergic receptor, alpha 2a; adrenergic, alpha-2A-, receptor; Alpha-2 adrenergic receptor subtype C10; alpha-2-adrenergic receptor, platelet type; Alpha-2A adrenergic receptor; Alpha-2A adrenoceptor; Alpha-2A adrenoreceptor; alpha-2AAR; alpha-2AAR subtype C10; Alpha-2D adrenergic receptor; alpha2A-adrenergic receptor; CA2-47; OTTHUMP00000058849; subtype alpha2-C10

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Gene Aliases: Adra-2; Adra-2a; ADRA2; ADRA2A; ADRA2R; ADRAR; alpha(2A)AR; alpha2-C10; alpha2A; alpha2A-AR; ALPHA2AAR; AW122659; CA2-47; RATRG20; RG20; ZNF32

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UniProt ID: (Human) P08913, (Mouse) Q01338, (Rat) P22909

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Entrez Gene ID: (Human) 150, (Mouse) 11551, (Rat) 25083

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Function(s)

alpha2-adrenergic receptor activity protein binding protein kinase binding alpha-1B adrenergic receptor binding alpha-2C adrenergic receptor binding thioesterase binding heterotrimeric G-protein binding protein homodimerization activity protein heterodimerization activity epinephrine binding norepinephrine binding adrenergic receptor activity

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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alpha-2a Adrenergic Receptor Polyclonal Antibody

alpha-2a Adrenergic Receptor Antibody (PA1-048) in WB

Product Details

PA1-048

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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