Invitrogen

alpha Actinin 2/3 Polyclonal Antibody

View all (3) alpha Actinin 2/3 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-99425

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human ACTN2(Accession P35609), corresponding to amino acid residues K38-D88.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2818358

Product Specific Information

Antibody detects endogenous levels of total Actinin alpha 2/3.

Target Information

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Actinin alpha3; actinin, alpha 2; actinin, alpha 3; Actinin-alpha3; alpha-actinin skeletal muscle; Alpha-actinin skeletal muscle isoform 2; Alpha-actinin skeletal muscle isoform 3; Alpha-actinin-2; Alpha-actinin-3; alpha-Actinin3; alphaActinin-3; F-actin cross-linking protein

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Gene Aliases: 1110008F24Rik; ACTN2; ACTN3; CMD1AA; CMH23

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UniProt ID: (Human) P35609, (Human) Q08043, (Mouse) Q9JI91, (Mouse) O88990

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Entrez Gene ID: (Human) 88, (Human) 89, (Mouse) 11472, (Rat) 291245, (Mouse) 11474, (Rat) 171009

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Function(s)
Ras guanyl-nucleotide exchange factor activity integrin binding calcium ion binding protein binding phosphatidylinositol-4,5-bisphosphate binding cytoskeletal protein binding structural constituent of muscle protein domain specific binding LIM domain binding thyroid hormone receptor coactivator activity titin binding identical protein binding protein homodimerization activity ion channel binding protein dimerization activity actin filament binding FATZ binding titin Z domain binding actin binding ligand-dependent nuclear receptor transcription coactivator activity protein binding, bridging metal ion binding
Process(es)
MAPK cascade platelet degranulation cell adhesion microspike assembly muscle filament sliding regulation of membrane potential regulation of apoptotic process negative regulation of potassium ion transport positive regulation of potassium ion transport positive regulation of GTPase activity sarcomere organization focal adhesion assembly protein homotetramerization actin filament uncapping cardiac muscle cell development phospholipase C-activating angiotensin-activated signaling pathway establishment of protein localization to plasma membrane negative regulation of potassium ion transmembrane transporter activity positive regulation of potassium ion transmembrane transporter activity regulation of nucleic acid-templated transcription negative regulation of protein localization to cell surface positive regulation of receptor activity positive regulation of endocytic recycling positive regulation of cation channel activity regulation of the force of skeletal muscle contraction skeletal muscle atrophy transition between fast and slow fiber response to denervation involved in regulation of muscle adaptation negative regulation of glycolytic process positive regulation of skeletal muscle tissue growth negative regulation of calcineurin-NFAT signaling cascade negative regulation of oxidative phosphorylation regulation of aerobic respiration positive regulation of glucose catabolic process to lactate via pyruvate muscle contraction
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