Invitrogen

alpha Actinin 2 Recombinant Rabbit Monoclonal Antibody (7H1L69)

alpha Actinin 2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 8

alpha Actinin 2 Antibody (701914) in ICC/IF

For immunofluorescence analysis, HSkM cells were fixed and permeabilized for detection of endogenous sarcomeric alpha actinin using Anti-sarcomeric alpha actinin Recombinant Rabbit Monoclonal Antibody (Product # 701914, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2,000). Panel a) shows representative cells that... View More

alpha Actinin 2 Antibody in Immunohistochemistry (Frozen) (IHC (F))

alpha Actinin 2 Antibody in Western Blot (WB)

alpha Actinin 2 Antibody in Western Blot, Immunoprecipitation (WB, IP)

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Product Details

701914

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

2 µg/mL

Immunocytochemistry (ICC/IF)

1:100

View 3 publications 3 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

7H1L69

Immunogen

Peptides corresponding to human Sarcomeric alpha actinin/alpha actinin 2 [1) aa834-aa852, 2) aa5-aa24]

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

AB_2688290

Product Specific Information

This antibody is predicted to react with Monkey, Pig, Rat, Mouse

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it i involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Induction of filopodia formation by α-Actinin-2 via RelA with a feedforward activation loop promoting overt bone marrow metastasis of gastric cancer.

Journal of translational medicine

Wang C,Xie B,Yin S,Cao J,Huang J,Jin L,Du G,Zhai X,Zhang R,Li S,Cao T,Yu H,Fan X,Yang Z,Peng J,Xiao J,Lian L

701914 was used in Immunocytochemistry-immunofluorescence to propose ACTN2 as a druggable molecular vulnerability that may provide potential therapeutic benefit against BMM of GC.

Mon Jun 19 00:00:00 UTC 2023

Induction of filopodia formation by α-Actinin-2 via RelA with a feedforward activation loop promoting overt bone marrow metastasis of gastric cancer.

Journal of translational medicine

Wang C,Xie B,Yin S,Cao J,Huang J,Jin L,Du G,Zhai X,Zhang R,Li S,Cao T,Yu H,Fan X,Yang Z,Peng J,Xiao J,Lian L

701914 was used in Immunocytochemistry-immunofluorescence to propose ACTN2 as a druggable molecular vulnerability that may provide potential therapeutic benefit against BMM of GC.

Mon Jun 19 00:00:00 UTC 2023

Induction of filopodia formation by α-Actinin-2 via RelA with a feedforward activation loop promoting overt bone marrow metastasis of gastric cancer.

Journal of translational medicine

Wang C,Xie B,Yin S,Cao J,Huang J,Jin L,Du G,Zhai X,Zhang R,Li S,Cao T,Yu H,Fan X,Yang Z,Peng J,Xiao J,Lian L

701914 was used in Immunocytochemistry-immunofluorescence to propose ACTN2 as a druggable molecular vulnerability that may provide potential therapeutic benefit against BMM of GC.

Mon Jun 19 00:00:00 UTC 2023

Hyperglycaemia-induced impairment of the autorhythmicity and gap junction activity of mouse embryonic stem cell-derived cardiomyocyte-like cells.

Histochemistry and cell biology

Menzele A,Aboalgasm H,Ballo R,Gwanyanya A

701914 was used in Immunocytochemistry to investigate the effect of hyperglycaemia on the pulsatile activity of stem cell-derived cardiomyocytes.

Sat Apr 01 00:00:00 UTC 2023

A multi-organ chip with matured tissue niches linked by vascular flow.

Nature biomedical engineering

Ronaldson-Bouchard K,Teles D,Yeager K,Tavakol DN,Zhao Y,Chramiec A,Tagore S,Summers M,Stylianos S,Tamargo M,Lee BM,Halligan SP,Abaci EH,Guo Z,Jacków J,Pappalardo A,Shih J,Soni RK,Sonar S,German C,Christiano AM,Califano A,Hirschi KK,Chen CS,Przekwas A,Vunjak-Novakovic G

701914 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot, Immunohistochemistry (Paraffin) to report the development and applicability of a tissue-chip system in which matured human heart, liver, bone and skin tissue niches are linked by recirculating vascular flow to allow for the recapitulation of interdependent organ functions.

Fri Apr 01 00:00:00 UTC 2022

Deep learning detects cardiotoxicity in a high-content screen with induced pluripotent stem cell-derived cardiomyocytes.

eLife

Grafton F,Ho J,Ranjbarvaziri S,Farshidfar F,Budan A,Steltzer S,Maddah M,Loewke KE,Green K,Patel S,Hoey T,Mandegar MA

701914 was used in Immunocytochemistry to screen a library of 1280 bioactive compounds and identified those with potential cardiotoxic liabilities in induced pluripotent stem cell-derived cardiomyocytes using a single-parameter score based on deep learning.

Mon Aug 02 00:00:00 UTC 2021

SK Channel Modulates Synaptic Plasticity by Tuning CaMKIIα/β Dynamics.

Frontiers in synaptic neuroscience

Shrestha A,Sultana R,Lee CC,Ogundele OM

701914 was used in Immunohistochemistry-immunofluorescence to demonstrate that positive modulation of SK channel at hippocampal synapses likely refine GluN1-linked plasticity by tuning dendritic localization of CaMKIIα.

Sun Apr 21 00:00:00 UTC 2024

Bioinformatics

Protein Aliases: actinin, alpha 2; alpha-actinin skeletal muscle; Alpha-actinin skeletal muscle isoform 2; Alpha-actinin-2; F-actin cross-linking protein

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Gene Aliases: 1110008F24Rik; ACTN2; CMD1AA; CMH23

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UniProt ID: (Human) P35609, (Mouse) Q9JI91

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Entrez Gene ID: (Human) 88, (Mouse) 11472, (Rat) 291245

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Function(s)

Ras guanyl-nucleotide exchange factor activity integrin binding calcium ion binding protein binding phosphatidylinositol-4,5-bisphosphate binding cytoskeletal protein binding structural constituent of muscle protein domain specific binding LIM domain binding thyroid hormone receptor coactivator activity titin binding identical protein binding protein homodimerization activity ion channel binding protein dimerization activity actin filament binding FATZ binding titin Z domain binding actin binding ligand-dependent nuclear receptor transcription coactivator activity protein binding, bridging metal ion binding

Process(es)

MAPK cascade platelet degranulation cell adhesion microspike assembly muscle filament sliding regulation of membrane potential regulation of apoptotic process negative regulation of potassium ion transport positive regulation of potassium ion transport positive regulation of GTPase activity sarcomere organization focal adhesion assembly protein homotetramerization actin filament uncapping cardiac muscle cell development phospholipase C-activating angiotensin-activated signaling pathway establishment of protein localization to plasma membrane negative regulation of potassium ion transmembrane transporter activity positive regulation of potassium ion transmembrane transporter activity regulation of nucleic acid-templated transcription negative regulation of protein localization to cell surface positive regulation of receptor activity positive regulation of endocytic recycling positive regulation of cation channel activity muscle contraction

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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alpha Actinin 2 Recombinant Rabbit Monoclonal Antibody (7H1L69)

alpha Actinin 2 Antibody (701914) in ICC/IF

Product Details

701914

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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