Proteintech

alpha Actinin Polyclonal Antibody

alpha Actinin Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 11

alpha Actinin Antibody (11313-2-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol) fixed C2C12 cell using 11313-2-AP (alpha Actinin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

alpha Actinin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

alpha Actinin Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

alpha Actinin Antibody in Western Blot (WB)

Product Image Gallery

Product Details

11313-2-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 15 publications 15 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 6 publications 6 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

alpha Actinin Fusion Protein Ag1859 (543-892 aa encoded by BC015766)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: EIQGLTTAH EQFKATLPDA DKERLAILGI HNEVSKIVQT YHVNMAGTNP YTTITPQEIN GKWDHVRQLV PRRDQALTEE HARQQHNERL RKQFGAQANV IGPWIQTKME EIGRISIEMH GTLEDQLSHL RQYEKSIVNY KPKIDQLEGD HQLIQEALIF DNKHTNYTME HIRVGWEQLL TTIARTINEV ENQILTRDAK GISQEQMNEF RASFNHFDRD HSGTLGPEEF KACLISLGYD IGNDPQGEAE FARIMSIVDP NRLGVVTFQA FIDFMSRETA DTDTADQVMA SFKILAGDKN YITMDELRRE LPPDQAEYCI ARMAPYTGPD SVPGALDYMS FSTALYGESD L (543-892 aa encoded by BC015766)

Target Information

Alpha Actinin 1 belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

MK-886 protects against cardiac ischaemia/reperfusion injury by activating proteasome-Keap1-NRF2 signalling.

Redox biology

Shi KN,Li PB,Su HX,Gao J,Li HH

Thu Jun 01 00:00:00 UTC 2023

Guanxinning injection ameliorates cardiac remodeling in HF mouse and 3D heart spheroid models via p38/FOS/MMP1-mediated inhibition of myocardial hypertrophy and fibrosis.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Fan S,Xiao G,Ni J,Zhao Y,Du H,Liang Y,Lv M,He S,Fan G,Zhu Y

Thu Jun 01 00:00:00 UTC 2023

Activation of long-non-coding RNA NEAT1 sponging microRNA-147 inhibits radiation damage by targeting PDPK1 in troxerutin radioprotection.

iScience

Hu YJ,Song GY,Zhang F,Zhang N,Wang F,Wang JL,Wang X,Wang TY,Li YF,Yan YD,Dou WT,Cheng CY,Xu P

Fri Feb 17 00:00:00 UTC 2023

DPY30 Promotes Proliferation and Cell Cycle Progression of Colorectal Cancer Cells via Mediating H3K4 Trimethylation.

International journal of medical sciences

Su WC,Mao XM,Li SY,Luo CY,Fan R,Jiang HF,Zhang LJ,Wang YT,Su GQ,Shen DY

Mon Jun 19 00:00:00 UTC 2023

CYP27A1 inhibits proliferation and migration of clear cell renal cell carcinoma via activation of LXRs/ABCA1.

Experimental cell research

Liang Z,Jiao W,Wang L,Chen Y,Li D,Zhang Z,Zhang Z,Liang Y,Niu H

Sat Oct 01 00:00:00 UTC 2022

MYPT1/PP1-Mediated EZH2 Dephosphorylation at S21 Promotes Epithelial-Mesenchymal Transition in Fibrosis through Control of Multiple Families of Genes.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Zhang L,Wang L,Hu XB,Hou M,Xiao Y,Xiang JW,Xie J,Chen ZG,Yang TH,Nie Q,Fu JL,Wang Y,Zheng SY,Liu YF,Gan YW,Gao Q,Bai YY,Wang JM,Qi RL,Zou M,Ke Q,Zhu XF,Gong L,Liu Y,Li DW

Sun May 01 00:00:00 UTC 2022

Inhibition of cGAS-STING by JQ1 alleviates oxidative stress-induced retina inflammation and degeneration.

Cell death and differentiation

Zou M,Ke Q,Nie Q,Qi R,Zhu X,Liu W,Hu X,Sun Q,Fu JL,Tang X,Liu Y,Li DW,Gong L

Thu Sep 01 00:00:00 UTC 2022

Calponin 1 contributes to myofibroblast differentiation of human pleural mesothelial cells.

American journal of physiology. Lung cellular and molecular physiology

Choo YY,Sakai T,Komatsu S,Ikebe R,Jeffers A,Singh KP,Idell S,Tucker TA,Ikebe M

Tue Mar 01 00:00:00 UTC 2022

A novel small compound TOIDC suppresses lipogenesis via SREBP1-dependent signaling to curb MAFLD.

Nutrition & metabolism

Shao Y,Yao Z,Zhou J,Yu M,Chen S,Yuan Y,Han L,Jiang L,Liu J

Tue Dec 06 00:00:00 UTC 2022

A ROS-Responsive Liposomal Composite Hydrogel Integrating Improved Mitochondrial Function and Pro-Angiogenesis for Efficient Treatment of Myocardial Infarction.

Advanced healthcare materials

Zheng Z,Lei C,Liu H,Jiang M,Zhou Z,Zhao Y,Yu CY,Wei H

Sat Oct 01 00:00:00 UTC 2022

Retinoic Acid Receptor Gamma (RARγ) Promotes Cartilage Destruction through Positive Feedback Activation of NF-κB Pathway in Human Osteoarthritis.

Mediators of inflammation

Yu YW,Li SY,Zhang LJ,Wang QL,Liu ZG,Chen QZ,Song HY,Shen DY,Yan J

Mon Nov 21 00:00:00 UTC 2022

CaMKII and Kalirin, a Rac1-GEF, regulate Akt phosphorylation involved in contraction-induced glucose uptake in skeletal muscle cells.

Biochemical and biophysical research communications

Liu S,Zhang J,Qi R,Deng B,Ni Y,Zhang C,Niu W

Sat Jun 25 00:00:00 UTC 2022

Identification and validation of an immune signature associated with EMT and metabolic reprogramming for predicting prognosis and drug response in bladder cancer.

Frontiers in immunology

Zhang Z,Yu Y,Li P,Wang M,Jiao W,Liang Y,Niu H

Mon Aug 15 00:00:00 UTC 2022

Disparate effects of MMP and TIMP modulation on coronary atherosclerosis and associated myocardial fibrosis.

Scientific reports

Kremastiotis G,Handa I,Jackson C,George S,Johnson J

Tue Nov 30 00:00:00 UTC 2021

Inhibitor of Differentiation-2 Protein Ameliorates DSS-Induced Ulcerative Colitis by Inhibiting NF-κB Activation in Neutrophils.

Frontiers in immunology

Ren J,Yan D,Wang Y,Zhang J,Li M,Xiong W,Jing X,Li P,Zhao W,Xiong X,Wu M,Zhong G

Thu Feb 17 00:00:00 UTC 2022

Aging-dependent loss of GAP junction proteins Cx46 and Cx50 in the fiber cells of human and mouse lenses accounts for the diminished coupling conductance.

Aging

Gong XD,Wang Y,Hu XB,Zheng SY,Fu JL,Nie Q,Wang L,Hou M,Xiang JW,Xiao Y,Gao Q,Bai YY,Liu YZ,Li DW

Sun Jul 04 00:00:00 UTC 2021

Different roles of BAG3 in cardiac physiological hypertrophy and pathological remodeling.

Translational research : the journal of laboratory and clinical medicine

Jia P,Wu N,Yang H,Guo Y,Guo X,Sun Y

Thu Jul 01 00:00:00 UTC 2021

Sirtuin 6 ameliorates alcohol-induced liver injury by reducing endoplasmic reticulum stress in mice.

Biochemical and biophysical research communications

Xin Y,Xu L,Zhang X,Yang C,Wang Q,Xiong X

Fri Mar 12 00:00:00 UTC 2021

Adipocyte-specific deletion of Depdc5 exacerbates insulin resistance and adipose tissue inflammation in mice.

Biochemical and biophysical research communications

Zhang J,Shi Y,Yang C,Sun B,Ma H,Huang R,Li Z,Wang M,Wang Q,Ma J,Xiong X

Fri Sep 10 00:00:00 UTC 2021

Dynamic spatiotemporal expression pattern of limbal stem cell putative biomarkers during mouse development.

Experimental eye research

Guo ZH,Zeng YM,Lin JS

Sun Mar 01 00:00:00 UTC 2020

miR-17-3p Contributes to Exercise-Induced Cardiac Growth and Protects against Myocardial Ischemia-Reperfusion Injury.

Theranostics

Shi J,Bei Y,Kong X,Liu X,Lei Z,Xu T,Wang H,Xuan Q,Chen P,Xu J,Che L,Liu H,Zhong J,Sluijter JP,Li X,Rosenzweig A,Xiao J

Mon Oct 23 00:00:00 UTC 2017

miR-17-3p Contributes to Exercise-Induced Cardiac Growth and Protects against Myocardial Ischemia-Reperfusion Injury.

Theranostics

Shi J,Bei Y,Kong X,Liu X,Lei Z,Xu T,Wang H,Xuan Q,Chen P,Xu J,Che L,Liu H,Zhong J,Sluijter JP,Li X,Rosenzweig A,Xiao J

Mon Oct 23 00:00:00 UTC 2017

Proteomic analysis for anti-atherosclerotic effect of tetrahydroxystilbene glucoside in rats.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Yao W,Fan W,Huang C,Zhong H,Chen X,Zhang W

Fri Mar 01 00:00:00 UTC 2013

Proteomic analysis for anti-atherosclerotic effect of tetrahydroxystilbene glucoside in rats.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Yao W,Fan W,Huang C,Zhong H,Chen X,Zhang W

Fri Mar 01 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: actinin 1 smooth muscle; actinin, alpha 1; alpha actinin 1a; Alpha-actinin cytoskeletal isoform; Alpha-actinin-1; F-actin cross-linking protein; FLJ40884; FLJ54432; non-muscle alpha-actinin 1; Non-muscle alpha-actinin-1

View more View less

Gene Aliases: 3110023F10Rik; ACTN1; Actn1a; BDPLT15

View more View less

UniProt ID: (Human) P12814, (Rat) Q9Z1P2, (Mouse) Q7TPR4

View more View less

Entrez Gene ID: (Human) 87, (Rat) 81634, (Mouse) 109711

View more View less

Function(s)

double-stranded RNA binding integrin binding calcium ion binding protein binding vinculin binding ligand-dependent nuclear receptor transcription coactivator activity protein homodimerization activity ion channel binding actin filament binding protein domain specific binding actin binding metal ion binding

Process(es)

platelet degranulation actin filament organization platelet activation platelet formation platelet morphogenesis regulation of apoptotic process focal adhesion assembly actin filament bundle assembly negative regulation of cellular component movement actin filament network formation actin crosslink formation platelet aggregation regulation of nucleic acid-templated transcription cortical cytoskeleton organization

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

alpha Actinin Polyclonal Antibody

alpha Actinin Antibody (11313-2-AP) in ICC/IF

Product Details

11313-2-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!