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Invitrogen
alpha Adducin Polyclonal Antibody
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FIGURE: 1 / 4
alpha Adducin Antibody (PA5-27460) in ICC/IF
Immunocytochemistry (Confocal immunofluorescence) of alpha Adducin in methanol-fixed A431. Samples were incubated with alpha Adducin polyclonal antibody (Product # PA5-27460) using a dilution of 1:500 (green). Alpha-tubulin filaments were labeled with alpha Tubulin monoclonal antibody (Product # MA1-25054) (Red) at 1:2,000.
Product Details
PA5-27460
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human alpha Adducin. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1.02 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2544936
Product Specific Information
Recommended positive controls: 293T, A431, Jurakt, Raji.
Predicted reactivity: Mouse (95%), Rat (96%), Zebrafish (89%), Xenopus laevis (90%), Chicken (92%), Rhesus Monkey (99%), Bovine (96%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ADD2; ADDB; adducin 1 (alpha); Adducin beta; Adducin-1; Adducin-alpha; Adducin-beta; Adducin1; Alpha adducin; Alpha-adducin; beta Adducin; Beta-adducin; erythrocyte adducin alpha subunit; Erythrocyte adducin subunit alpha
Gene Aliases: ADD1; ADDA; AI256389
UniProt ID: (Human) P35611, (Mouse) Q9QYC0
Entrez Gene ID: (Human) 118, (Mouse) 11518
cell morphogenesis
in utero embryonic development
cell volume homeostasis
hemoglobin metabolic process
erythrocyte differentiation
negative regulation of actin filament polymerization
positive regulation of protein binding
multicellular organism growth
positive regulation of angiogenesis
positive regulation of endocytosis
homeostasis of number of cells within a tissue
barbed-end actin filament capping
actin filament bundle assembly
cellular response to retinoic acid
actin cytoskeleton organization
IRE1-mediated unfolded protein response
transmembrane transport
cell-cell adhesion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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