Invitrogen

alpha Synuclein Monoclonal Antibody (Syn 211)

alpha Synuclein Antibody in Immunocytochemistry (ICC/IF)

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alpha Synuclein Antibody (MA5-12272) in ICC/IF

Immunofluorescent analysis of Synuclein (green) showing staining in the cytoplasm and membrane of U87-MG cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Synuclein monoclonal antibody (Product # MA5-12272) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC i... View More

alpha Synuclein Antibody in Immunohistochemistry (Paraffin) (IHC (P))

alpha Synuclein Antibody in Western Blot (WB)

alpha Synuclein Antibody in Immunohistochemistry (IHC)

alpha Synuclein Antibody in Proximity Ligation Assay (PLA) (PLA)

Product Image Gallery

Product Details

MA5-12272

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:400

Immunocytochemistry (ICC/IF)

1:20-1:200

View 7 publications 7 publications

Immunoprecipitation (IP)

Assay-dependent

in situ PLA (PLA)

View 1 publication 1 publication

Immunomicroscopy (IM)

View 1 publication 1 publication

In vitro Assay (IV)

View 2 publications 2 publications

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

Syn 211

Immunogen

Human recombinant alpha-synuclein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10978319

Product Specific Information

MA5-12272 targets Synuclein in IHC (P), ICC/IF, IP, and WB applications and shows reactivity with Human and Rat samples.

The MA5-12272 immunogen is human recombinant alpha-synuclein.

Target Information

Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. These proteins are abundant in the brain and play a role in regulating synaptic transmission and membrane trafficking. Alpha- and beta-synuclein have been shown to selectively inhibit phospholipase D2, suggesting that they may also be involved in signaling pathways. Defects in the SNCA gene, which encodes alpha-synuclein, have been implicated in the pathogenesis of Parkinson's disease. Additionally, alpha-synuclein peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Multiple alternatively spliced transcripts encoding different isoforms have been identified for this gene. Diseases associated with SNCA include Parkinson Disease 1, Autosomal Dominant and Dementia, Lewy Body.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

N-homocysteinylation of α-synuclein promotes its aggregation and neurotoxicity.

Aging cell

Zhou L,Guo T,Meng L,Zhang X,Tian Y,Dai L,Niu X,Li Y,Liu C,Chen G,Liu C,Ke W,Zhang Z,Bao A,Zhang Z

MA5-12272 was used in Immunohistochemistry to suggest that the covalent modification of α-synuclein by HTL promotes its aggregation.

Wed Mar 01 00:00:00 UTC 2023

N-homocysteinylation of α-synuclein promotes its aggregation and neurotoxicity.

Aging cell

Zhou L,Guo T,Meng L,Zhang X,Tian Y,Dai L,Niu X,Li Y,Liu C,Chen G,Liu C,Ke W,Zhang Z,Bao A,Zhang Z

MA5-12272 was used in Immunohistochemistry to suggest that the covalent modification of α-synuclein by HTL promotes its aggregation.

Wed Mar 01 00:00:00 UTC 2023

Angiotensin-(1-7) reduces α-synuclein aggregation by enhancing autophagic activity in Parkinson's disease.

Neural regeneration research

Gao Q,Chen R,Wu L,Huang Q,Wang XX,Tian YY,Zhang YD

MA5-12272 was used in Immunocytochemistry-immunofluorescence to conclude that angiotensin-(1-7) reduces α-synuclein aggregation by alleviating autophagy dysfunction in Parkinson's disease.

Sun May 01 00:00:00 UTC 2022

Angiotensin-(1-7) reduces α-synuclein aggregation by enhancing autophagic activity in Parkinson's disease.

Neural regeneration research

Gao Q,Chen R,Wu L,Huang Q,Wang XX,Tian YY,Zhang YD

MA5-12272 was used in Immunocytochemistry-immunofluorescence to conclude that angiotensin-(1-7) reduces α-synuclein aggregation by alleviating autophagy dysfunction in Parkinson's disease.

Sun May 01 00:00:00 UTC 2022

Fine Particulate Matter Triggers α-Synuclein Fibrillization and Parkinson-like Neurodegeneration.

Movement disorders : official journal of the Movement Disorder Society

Yuan X,Yang Y,Liu C,Tian Y,Xia D,Liu Z,Pan L,Xiong M,Xiong J,Meng L,Zhang Z,Ye K,Jiang H,Zhang Z

MA5-12272 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, In Vitro Assay to demonstrate that PM2.5 exposure promotes a-Syn pathology, promoting neuronal degeneration and increasing the risk for PD.

Thu Sep 01 00:00:00 UTC 2022

Fine Particulate Matter Triggers α-Synuclein Fibrillization and Parkinson-like Neurodegeneration.

Movement disorders : official journal of the Movement Disorder Society

Yuan X,Yang Y,Liu C,Tian Y,Xia D,Liu Z,Pan L,Xiong M,Xiong J,Meng L,Zhang Z,Ye K,Jiang H,Zhang Z

Thu Sep 01 00:00:00 UTC 2022

Cofilin 1 promotes the pathogenicity and transmission of pathological α-synuclein in mouse models of Parkinson's disease.

NPJ Parkinson's disease

Yan M,Xiong M,Dai L,Zhang X,Zha Y,Deng X,Yu Z,Zhang Z

MA5-12272 was used in Immunomicroscopy to illustrate the important role of cofilin 1 in the pathogenicity and transmission of α-synuclein during the onset and progression of Parkinson's disease.

Mon Jan 10 00:00:00 UTC 2022

Mechanism assay of interaction between blood vessels-near infrared probe and cell surface marker proteins of endothelial cells.

Materials today. Bio

Abdul Sisak MA,Louis F,Miyao T,Lee SH,Chang YT,Matsusaki M

MA5-12272 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, In Vitro Assay to study i the competition assays of CyA-B2 using several potential surface markers of endothelial cells found through the chemical database (ChEMBL) and manually selected, CD133 gave the lowest IC50 (half maximal inhibitory concentration) value.

Wed Jun 01 00:00:00 UTC 2022

Alpha-Synuclein in the Regulation of Brain Endothelial and Perivascular Cells: Gaps and Future Perspectives.

Frontiers in immunology

Bogale TA,Faustini G,Longhena F,Mitola S,Pizzi M,Bellucci A

MA5-12272 was used in Immunocytochemistry to summarize the main findings supporting how α-synuclein can affect ECs and/or BAMs function as well as their interplay and effect on other cells in the brain perivascular environment in physiological and pathological conditions.

Wed Jun 30 00:00:00 UTC 2021

Alpha-Synuclein in the Regulation of Brain Endothelial and Perivascular Cells: Gaps and Future Perspectives.

Frontiers in immunology

Bogale TA,Faustini G,Longhena F,Mitola S,Pizzi M,Bellucci A

Wed Jun 30 00:00:00 UTC 2021

Alpha-synuclein is involved in manganese-induced spatial memory and synaptic plasticity impairments via TrkB/Akt/Fyn-mediated phosphorylation of NMDA receptors.

Cell death & disease

Ma Z,Liu K,Li XR,Wang C,Liu C,Yan DY,Deng Y,Liu W,Xu B

MA5-12272 was used in Western Blotting to whether Mn-dependent enhanced alpha-synuclein expression accounts for interference with BDNF/TrkB signaling.

Thu Oct 08 00:00:00 UTC 2020

Alpha-synuclein is involved in manganese-induced spatial memory and synaptic plasticity impairments via TrkB/Akt/Fyn-mediated phosphorylation of NMDA receptors.

Cell death & disease

Ma Z,Liu K,Li XR,Wang C,Liu C,Yan DY,Deng Y,Liu W,Xu B

MA5-12272 was used in Western Blotting to whether Mn-dependent enhanced alpha-synuclein expression accounts for interference with BDNF/TrkB signaling.

Thu Oct 08 00:00:00 UTC 2020

Dopamine Transporter/α-Synuclein Complexes Are Altered in the Post Mortem Caudate Putamen of Parkinson's Disease: An In Situ Proximity Ligation Assay Study.

International journal of molecular sciences

Longhena F,Faustini G,Missale C,Pizzi M,Bellucci A

MA5-12272 was used in Immunohistochemistry to investigate dopamine transporter/α-synuclein complexes in the caudate putamen of Parkinson's disease patients and age-matched controls.

Wed May 30 00:00:00 UTC 2018

Dopamine Transporter/α-Synuclein Complexes Are Altered in the Post Mortem Caudate Putamen of Parkinson's Disease: An In Situ Proximity Ligation Assay Study.

International journal of molecular sciences

Longhena F,Faustini G,Missale C,Pizzi M,Bellucci A

MA5-12272 was used in Immunohistochemistry to investigate dopamine transporter/α-synuclein complexes in the caudate putamen of Parkinson's disease patients and age-matched controls.

Wed May 30 00:00:00 UTC 2018

Dopamine Transporter/α-Synuclein Complexes Are Altered in the Post Mortem Caudate Putamen of Parkinson's Disease: An In Situ Proximity Ligation Assay Study.

International journal of molecular sciences

Longhena F,Faustini G,Missale C,Pizzi M,Bellucci A

MA5-12272 was used in Immunohistochemistry to investigate dopamine transporter/α-synuclein complexes in the caudate putamen of Parkinson's disease patients and age-matched controls.

Wed May 30 00:00:00 UTC 2018

Lack of pathogenic potential of peripheral α-synuclein aggregates from Parkinson's disease patients.

Acta neuropathologica communications

Recasens A,Carballo-Carbajal I,Parent A,Bové J,Gelpi E,Tolosa E,Vila M

MA5-12272 was used in Immunohistochemistry to study the pathogenic capacity of Parkinson's disease-derived a-synuclein aggregates.

Thu Feb 08 00:00:00 UTC 2018

Cortical phosphorylated α-Synuclein levels correlate with brain wave spectra in Parkinson's disease.

Movement disorders : official journal of the Movement Disorder Society

Caviness JN,Lue LF,Hentz JG,Schmitz CT,Adler CH,Shill HA,Sabbagh MN,Beach TG,Walker DG

MA5-12272 was used in western blot to analyze the correlation between brain wave spectra in Parkinson's disease and cortical phosphorylated alpha-synuclein levels

Fri Jul 01 00:00:00 UTC 2016

Neurite Aggregation and Calcium Dysfunction in iPSC-Derived Sensory Neurons with Parkinson's Disease-Related LRRK2 G2019S Mutation.

Stem cell reports

Schwab AJ,Ebert AD

MA5-12272 was used in immunocytochemistry to study the effect of G2019S on neuronal subtypes

Tue Dec 08 00:00:00 UTC 2015

Lack of additive role of ageing in nigrostriatal neurodegeneration triggered by α-synuclein overexpression.

Acta neuropathologica communications

Bourdenx M,Dovero S,Engeln M,Bido S,Bastide MF,Dutheil N,Vollenweider I,Baud L,Piron C,Grouthier V,Boraud T,Porras G,Li Q,Baekelandt V,Scheller D,Michel A,Fernagut PO,Georges F,Courtine G,Bezard E,Dehay B

MA5-12272 was used in immunohistochemistry to study alpha-synuclein overexpression by lack of an additive role of aging in nigrostriatal neurodegeneration

Sat Jul 25 00:00:00 UTC 2015

Hypoestoxide reduces neuroinflammation and α-synuclein accumulation in a mouse model of Parkinson's disease.

Journal of neuroinflammation

Kim C,Ojo-Amaize E,Spencer B,Rockenstein E,Mante M,Desplats P,Wrasidlo W,Adame A,Nchekwube E,Oyemade O,Okogun J,Chan M,Cottam H,Masliah E

MA5-12272 was used in immunohistochemistry to study a mouse model of Parkinson's disease for hypoestoxide to reduce neuroinflammation and alpha-synuclein accumulation

Fri Dec 18 00:00:00 UTC 2015

A brain-targeted, modified neurosin (kallikrein-6) reduces α-synuclein accumulation in a mouse model of multiple system atrophy.

Molecular neurodegeneration

Spencer B,Valera E,Rockenstein E,Trejo-Morales M,Adame A,Masliah E

MA5-12272 was used in western blot to reduce alpha-synuclein accumulation in a mouse model of multiple system atrophy by using a brain-targeted, modified neurosin (kallikrein-6)

Wed Sep 23 00:00:00 UTC 2015

Differential expression of alpha-synuclein in hippocampal neurons.

PloS one

Taguchi K,Watanabe Y,Tsujimura A,Tatebe H,Miyata S,Tokuda T,Mizuno T,Tanaka M

MA5-12272 was used in immunocytochemistry to study the hippocampal neuronal expression of alpha-synuclein under normal and pathological conditions

Mon Dec 22 00:00:00 UTC 2014

α-Synuclein membrane association is regulated by the Rab3a recycling machinery and presynaptic activity.

The Journal of biological chemistry

Chen RHC,Wislet-Gendebien S,Samuel F,Visanji NP,Zhang G,Marsilio D,Langman T,Fraser PE,Tandon A

MA5-12272 was used in western blot to study the role of Rab3a and synaptic activity in the membrane association of alpha-synuclein

Fri Mar 15 00:00:00 UTC 2013

α-synuclein phosphorylation and truncation are normal events in the adult human brain.

Neuroscience

Muntané G,Ferrer I,Martinez-Vicente M

MA5-12272 was used in western blot to study the constitutive truncation and phosphorylation of alpha-synuclein in normal human brain

Tue Jan 03 00:00:00 UTC 2012

Neuropathology in mice expressing mouse alpha-synuclein.

PloS one

Rieker C,Dev KK,Lehnhoff K,Barbieri S,Ksiazek I,Kauffmann S,Danner S,Schell H,Boden C,Ruegg MA,Kahle PJ,van der Putten H,Shimshek DR

MA5-12272 was used in western blot to characterize mice that over express murine alpha-Synuclein

Thu Mar 01 00:00:00 UTC 2012

Alpha-synuclein mediates alterations in membrane conductance: a potential role for alpha-synuclein oligomers in cell vulnerability.

The European journal of neuroscience

Feng LR,Federoff HJ,Vicini S,Maguire-Zeiss KA

MA5-12272 was used in immunocytochemistry to study direct synuclein-induced cell membrane changes

Thu Jul 01 00:00:00 UTC 2010

Identification of glutathione S-transferase pi as a protein involved in Parkinson disease progression.

The American journal of pathology

Shi M,Bradner J,Bammler TK,Eaton DL,Zhang J,Ye Z,Wilson AM,Montine TJ,Pan C,Zhang J

MA5-12272 was used in Immunohistochemistry to study the role of the levels of glutathione S-transferase Pi in modulating the progression of Parkinson's disease.

Wed Jul 01 00:00:00 UTC 2009

A reassessment of the neuropathology of frontotemporal dementia linked to chromosome 3.

Journal of neuropathology and experimental neurology

Holm IE,Englund E,Mackenzie IR,Johannsen P,Isaacs AM

MA5-12272 was used in immunohistochemistry to re-evaluate the neuropathology of frontotemporal dementia linked to chromosome 3 in a Danish family

Mon Oct 01 00:00:00 UTC 2007

Abnormal alpha-synuclein solubility, aggregation and nitration in the frontal cortex in Pick's disease.

Neuroscience letters

Dalfó E,Martinez A,Muntané G,Ferrer I

MA5-12272 was used in western blot to study the distinct changes in the solubility, aggregation and tyrosine nitration of alpha-synuclein observed in the frontal cortex in Pick' disease

Mon May 29 00:00:00 UTC 2006

Bioinformatics

Protein Aliases: Alpha synuclein; Alpha-synuclein; NACP; non A-beta component of AD amyloid; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; synuclein alpha-140; synuclein, alpha (non A4 component of amyloid precursor)

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Gene Aliases: NACP; PARK1; PARK4; PD1; SNCA

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UniProt ID: (Human) P37840, (Rat) P37377

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Entrez Gene ID: (Human) 6622, (Rat) 29219

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Function(s)

magnesium ion binding fatty acid binding copper ion binding calcium ion binding protein binding phospholipid binding microtubule binding ferrous iron binding zinc ion binding oxidoreductase activity kinesin binding protein domain specific binding histone binding identical protein binding alpha-tubulin binding cysteine-type endopeptidase inhibitor activity involved in apoptotic process phospholipase binding transcription regulatory region DNA binding dynein binding protein N-terminus binding tau protein binding beta-tubulin binding phosphoprotein binding phospholipase D inhibitor activity enzyme binding Hsp70 protein binding arachidonic acid binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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alpha Synuclein Monoclonal Antibody (Syn 211)

alpha Synuclein Antibody (MA5-12272) in ICC/IF

Product Details

MA5-12272

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

in situ PLA (PLA)

Immunomicroscopy (IM)

In vitro Assay (IV)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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