Invitrogen

alpha Tubulin Monoclonal Antibody (DM1A), eBioscience™

11 Published Figures

28 References

View all (82) alpha Tubulin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)
Published Figures (11)

alpha Tubulin Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 13

alpha Tubulin Antibody (14-4502-82) in ICC/IF

Immunofluorescent analysis of alpha tubulin (green) in a BHP 2-7 thyroid cancer anaphase cell cultured in complete medium on a glass slide. Cells were fixed with 4% phosphate-buffered paraformaldehyde for 30 min at 37C, permeabilized with 100% ice-cold methanol for 20 min. and blocked with PBS/1% BSA/0.5% Tween 20 at 37C for 30 min. Cells were stained with monoclonal alpha tubulin antibody (Product # 14-45... View More

alpha Tubulin Antibody in Western Blot (WB)

alpha Tubulin Antibody in Immunocytochemistry, Proximity Ligation Assay (PLA) (ICC/IF, PLA)

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Product Details

14-4502-82

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 17 publications 17 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

Immunocytochemistry (ICC/IF)

Assay-Dependent

View 8 publications 8 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-Dependent

in situ PLA (PLA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Human, Mouse, Non-human primate, Pig, Rat

Published species

Dog, Human, Mouse, Non-human primate, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

DM1A

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_1210456

Product Specific Information

Description: The monoclonal antibody DM1a recognizes the 50 kDa cytoskeletal protein alpha tubulin in a variety of species (human, mouse, rat, monkey, dog, pig, bovine, goat, hamster, guinea pig, kangaroo, amphibians, sea urchin, yeast and tobacco plants). Tubulin, the major component of microtubules, is a dimeric protein consisting of an alpha and beta subunit. Tubulin is a GTP-binding protein that can be modified by phosphorylation and acetylation resulting in assembly (polymerization) or disassembly (depolymerization). The dynamic nature of microtubules is most evident in the mitotic apparatus. The DM1a antibody recognizes the C-terminal end of the alpha tubulin isoform (amino acids 426-430).

Applications Reported: This DM1A antibody has been reported for use in immunocytochemistry, immunoprecipitation, western blotting, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.

Applications Tested: This DM1A antibody has been tested by western blotting under reducing conditions (at 2 µg/mL) or by immunofluorescent staining of paraformaldehyde fixed and permeabilized cells.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Atomic force microscopy identifies the alteration of rheological properties of the cardiac fibroblasts in idiopathic restrictive cardiomyopathy.

PloS one

Matsumoto M,Tsuru H,Suginobe H,Narita J,Ishii R,Hirose M,Hashimoto K,Wang R,Yoshihara C,Ueyama A,Tanaka R,Ozono K,Okajima T,Ishida H

14-4502-82 was used in Immunocytochemistry and Immunohistochemistry to evaluate the rheological properties of cardiac fibroblasts (CFs) derived from pediatric patients with RCM and healthy control CFs via atomic force microscopy.

Mon Oct 03 00:00:00 UTC 2022

Atomic force microscopy identifies the alteration of rheological properties of the cardiac fibroblasts in idiopathic restrictive cardiomyopathy.

PloS one

Matsumoto M,Tsuru H,Suginobe H,Narita J,Ishii R,Hirose M,Hashimoto K,Wang R,Yoshihara C,Ueyama A,Tanaka R,Ozono K,Okajima T,Ishida H

Mon Oct 03 00:00:00 UTC 2022

Engrailed 1 coordinates cytoskeletal reorganization to induce myofibroblast differentiation.

The Journal of experimental medicine

Györfi AH,Matei AE,Fuchs M,Liang C,Rigau AR,Hong X,Zhu H,Luber M,Bergmann C,Dees C,Ludolph I,Horch RE,Distler O,Wang J,Bengsch B,Schett G,Kunz M,Distler JHW

14-4502-82 was used in Western Blot to show that Engrailed 1 (EN1) is reexpressed in multiple fibroblast subpopulations in the skin of SSc patients.

Mon Sep 06 00:00:00 UTC 2021

Rapid degradation of GRASP55 and GRASP65 reveals their immediate impact on the Golgi structure.

The Journal of cell biology

Zhang Y,Seemann J

14-4502-82 was used in Immunocytochemistry to dissect the respective roles of GRASP55 and GRASP65 on golgi structure formation.

Mon Jan 04 00:00:00 UTC 2021

Rapid degradation of GRASP55 and GRASP65 reveals their immediate impact on the Golgi structure.

The Journal of cell biology

Zhang Y,Seemann J

14-4502-82 was used in Immunocytochemistry to dissect the respective roles of GRASP55 and GRASP65 on golgi structure formation.

Mon Jan 04 00:00:00 UTC 2021

Angulin-1 seals tricellular contacts independently of tricellulin and claudins.

The Journal of cell biology

Sugawara T,Furuse K,Otani T,Wakayama T,Furuse M

14-4502-82 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to investigate the roles of angulin-1 and tricellulin in tTJ formation in MDCK II cells by genome editing. Angulin-1-deficient cells lost the plasma membrane contact at TCs with impaired epithelial barrier function.

Mon Sep 06 00:00:00 UTC 2021

Targeting EML4-ALK gene fusion variant 3 in thyroid cancer.

Endocrine-related cancer

Aydemirli MD,van Eendenburg JDH,van Wezel T,Oosting J,Corver WE,Kapiteijn E,Morreau H

14-4502-82 was used in Western Blot to the first reported case describing a patient-specific targeted treatment with lorlatinib based on an EML4-ALK gene fusion v3 in a thyroid cancer patient, and own cancer cell line.

Tue May 11 00:00:00 UTC 2021

Targeting EML4-ALK gene fusion variant 3 in thyroid cancer.

Endocrine-related cancer

Aydemirli MD,van Eendenburg JDH,van Wezel T,Oosting J,Corver WE,Kapiteijn E,Morreau H

Tue May 11 00:00:00 UTC 2021

Androstano-arylpyrimidines: Novel small molecule inhibitors of MDR1 for sensitizing multidrug-resistant breast cancer cells.

European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences

Gopisetty MK,Adamecz DI,Nagy FI,Baji Á,Lathira V,Szabó MR,Gáspár R,Csont T,Frank É,Kiricsi M

14-4502-82 was used in Western Blot to investigate if novel dihydrotestosterone-derived A-ring-fused arylpyrimidinone derivatives, based on their acetylation status, can inhibit multidrug resistance gene 1 (MDR1) efflux activity in MDR1 overexpressing multidrug-resistant breast adenocarcinoma cells.

Fri Jan 01 00:00:00 UTC 2021

Giant ankyrin-B suppresses stochastic collateral axon branching through direct interaction with microtubules.

The Journal of cell biology

Chen K,Yang R,Li Y,Zhou JC,Zhang M

Published figure using alpha Tubulin monoclonal antibody (Product # 14-4502-82) in Immunocytochemistry

Mon Aug 03 00:00:00 UTC 2020

Loss of the Nuclear Protein RTF2 Enhances Influenza Virus Replication.

Journal of virology

Chia BS,Li B,Cui A,Eisenhaure T,Raychowdhury R,Lieb D,Hacohen N

14-4502 was used in Western Blotting to screen for factors which limit IAV replication in response to IFN-ß in lung epithelial cells and investigate th effects of RTF2 on influenza virus replication.

Tue Oct 27 00:00:00 UTC 2020

Loss of the Nuclear Protein RTF2 Enhances Influenza Virus Replication.

Journal of virology

Chia BS,Li B,Cui A,Eisenhaure T,Raychowdhury R,Lieb D,Hacohen N

Tue Oct 27 00:00:00 UTC 2020

PKCγ-Mediated Phosphorylation of CRMP2 Regulates Dendritic Outgrowth in Cerebellar Purkinje Cells.

Molecular neurobiology

Winkler SC,Shimobayashi E,Kapfhammer JP

14-4502-80 was used in Proximity Ligation Assay (PLA) to show that Vangl2 exerts a bimodal regulation on young and mature GCs, and its disruption leads to an imbalance in hippocampus-dependent pattern completion and separation processes.

Tue Dec 01 00:00:00 UTC 2020

PKCγ-Mediated Phosphorylation of CRMP2 Regulates Dendritic Outgrowth in Cerebellar Purkinje Cells.

Molecular neurobiology

Winkler SC,Shimobayashi E,Kapfhammer JP

Tue Dec 01 00:00:00 UTC 2020

PKCγ-Mediated Phosphorylation of CRMP2 Regulates Dendritic Outgrowth in Cerebellar Purkinje Cells.

Molecular neurobiology

Winkler SC,Shimobayashi E,Kapfhammer JP

Tue Dec 01 00:00:00 UTC 2020

GATA factor-regulated solute carrier ensemble reveals a nucleoside transporter-dependent differentiation mechanism.

PLoS genetics

Zwifelhofer NM,Cai X,Liao R,Mao B,Conn DJ,Mehta C,Keles S,Xia Y,Bresnick EH

Published figure using alpha Tubulin monoclonal antibody (Product # 14-4502-82) in Western Blot

Tue Dec 01 00:00:00 UTC 2020

Metabolic reprogramming related to whole-chromosome instability in models for Hürthle cell carcinoma.

Scientific reports

Addie RD,Kostidis S,Corver WE,Oosting J,Aminzadeh-Gohari S,Feichtinger RG,Kofler B,Aydemirli MD,Giera M,Morreau H

14-4502-82 was used in Western Blot to study shared metabolic traits during standard and glucose-depleted cell culture in thyroid cancer cell lines, with or without a near-homozygous genome.

Fri Jun 12 00:00:00 UTC 2020

Metabolic reprogramming related to whole-chromosome instability in models for Hürthle cell carcinoma.

Scientific reports

Addie RD,Kostidis S,Corver WE,Oosting J,Aminzadeh-Gohari S,Feichtinger RG,Kofler B,Aydemirli MD,Giera M,Morreau H

14-4502-82 was used in Western Blot to study shared metabolic traits during standard and glucose-depleted cell culture in thyroid cancer cell lines, with or without a near-homozygous genome.

Fri Jun 12 00:00:00 UTC 2020

The extracellular domain of angulin-1 and palmitoylation of its cytoplasmic region are required for angulin-1 assembly at tricellular contacts.

The Journal of biological chemistry

Oda Y,Sugawara T,Fukata Y,Izumi Y,Otani T,Higashi T,Fukata M,Furuse M

14-4502 was used in Western Blotting to examine the molecular mechanism for the assembly of angulin-1 at the tricellular contacts.

Fri Mar 27 00:00:00 UTC 2020

AAGAB Controls AP2 Adaptor Assembly in Clathrin-Mediated Endocytosis.

Developmental cell

Gulbranson DR,Crisman L,Lee M,Ouyang Y,Menasche BL,Demmitt BA,Wan C,Nomura T,Ye Y,Yu H,Shen J

14-4502-82 was used in Flow Cytometry to conclude that since other multimeric trafficking adaptors operate in an analogous manner to AP2 adaptor, their assembly likely involves a similar regulatory mechanism.

Mon Aug 19 00:00:00 UTC 2019

Palmitic acid induces guanylin gene expression through the Toll-like receptor 4/nuclear factor-κB pathway in rat macrophages.

American journal of physiology. Cell physiology

Akieda-Asai S,Ma H,Date Y

14-4502 was used in Western Blotting to indicate that saturated fatty acids, such as palmitic acid, activate guanylin gene expression via the nuclear factor-κB pathway in macrophages.

Sun Dec 01 00:00:00 UTC 2019

Endoplasmic reticulum stress: major player in size-dependent inhibition of P-glycoprotein by silver nanoparticles in multidrug-resistant breast cancer cells.

Journal of nanobiotechnology

Gopisetty MK,Kovács D,Igaz N,Rónavári A,Bélteky P,Rázga Z,Venglovecz V,Csoboz B,Boros IM,Kónya Z,Kiricsi M

14-4502 was used in Western Blotting to suggest that AgNPs are potent inhibitors of Pgp function and are promising agents for sensitizing multidrug resistant breast cancers to anticancer drugs.

Tue Jan 22 00:00:00 UTC 2019

MARCH6 and TRC8 facilitate the quality control of cytosolic and tail-anchored proteins.

EMBO reports

Stefanovic-Barrett S,Dickson AS,Burr SP,Williamson JC,Lobb IT,van den Boomen DJ,Lehner PJ,Nathan JA

14-4502 was used in Western Blotting to elucidate the roles of MARCH6 and TRC8 in facilitating the quality control of cytosolic and tail-anchored proteins.

Tue May 01 00:00:00 UTC 2018

Multiple Levels of Control Determine How E4bp4/Nfil3 Regulates NK Cell Development.

Journal of immunology (Baltimore, Md. : 1950)

Kostrzewski T,Borg AJ,Meng Y,Filipovic I,Male V,Wack A,DiMaggio PA,Brady HJM

Thu Feb 15 00:00:00 UTC 2018

A two-phase response of endothelial cells to hydrostatic pressure.

Journal of cell science

Prystopiuk V,Fels B,Simon CS,Liashkovich I,Pasrednik D,Kronlage C,Wedlich-Söldner R,Oberleithner H,Fels J

14-4502-82 was used in Western Blot to characterise the specific response of endothelial cells to application of pressure by using a combination of quantitative fluorescence microscopy, atomic force microscopy and molecular perturbations.

Thu Jun 21 00:00:00 UTC 2018

RABIF/MSS4 is a Rab-stabilizing holdase chaperone required for GLUT4 exocytosis.

Proceedings of the National Academy of Sciences of the United States of America

Gulbranson DR,Davis EM,Demmitt BA,Ouyang Y,Ye Y,Yu H,Shen J

14-4502 was used in Western Blotting to reveal a crucial role of RABIF in vesicle transport and establish RABIF as a Rab-stabilizing holdase chaperone.

Tue Sep 26 00:00:00 UTC 2017

RABIF/MSS4 is a Rab-stabilizing holdase chaperone required for GLUT4 exocytosis.

Proceedings of the National Academy of Sciences of the United States of America

Gulbranson DR,Davis EM,Demmitt BA,Ouyang Y,Ye Y,Yu H,Shen J

14-4502 was used in Western Blotting to reveal a crucial role of RABIF in vesicle transport and establish RABIF as a Rab-stabilizing holdase chaperone.

Tue Sep 26 00:00:00 UTC 2017

RABIF/MSS4 is a Rab-stabilizing holdase chaperone required for GLUT4 exocytosis.

Proceedings of the National Academy of Sciences of the United States of America

Gulbranson DR,Davis EM,Demmitt BA,Ouyang Y,Ye Y,Yu H,Shen J

14-4502 was used in Western Blotting to reveal a crucial role of RABIF in vesicle transport and establish RABIF as a Rab-stabilizing holdase chaperone.

Tue Sep 26 00:00:00 UTC 2017

The vacuolar-ATPase complex and assembly factors, TMEM199 and CCDC115, control HIF1α prolyl hydroxylation by regulating cellular iron levels.

eLife

Miles AL,Burr SP,Grice GL,Nathan JA

14450282 was used in western blot to identify cellular processes that regulate HIF1alpha

Wed Mar 15 00:00:00 UTC 2017

Do mechanical strain and TNF-α interact to amplify pro-inflammatory cytokine production in human annulus fibrosus cells?

Journal of biomechanics

Likhitpanichkul M,Torre OM,Gruen J,Walter BA,Hecht AC,Iatridis JC

Published figure using alpha Tubulin monoclonal antibody (Product # 14-4502-82) in Immunocytochemistry

Tue May 03 00:00:00 UTC 2016

Disruption of tumour-host communication by downregulation of LFA-1 reduces COX-2 and e-NOS expression and inhibits brain metastasis growth.

Oncotarget

Soto MS,O'Brien ER,Andreou K,Scrace SF,Zakaria R,Jenkinson MD,O'Neill E,Sibson NR

14-4502 was used in Western blot to examine whether cross-talk between tumour cells and host brain cells, through tumour cell leukocyte function associated protein-1, is critical in metastasis development.

Tue Aug 09 00:00:00 UTC 2016

Comparative Haploid Genetic Screens Reveal Divergent Pathways in the Biogenesis and Trafficking of Glycophosphatidylinositol-Anchored Proteins.

Cell reports

Davis EM,Kim J,Menasche BL,Sheppard J,Liu X,Tan AC,Shen J

14-4502 was used in Western Blotting to characterise the biogenesis of GPI-anchored proteins.

Tue Jun 23 00:00:00 UTC 2015

IP-10/CXCL10 induction in human pancreatic cancer stroma influences lymphocytes recruitment and correlates with poor survival.

Oncotarget

Lunardi S,Jamieson NB,Lim SY,Griffiths KL,Carvalho-Gaspar M,Al-Assar O,Yameen S,Carter RC,McKay CJ,Spoletini G,D'Ugo S,Silva MA,Sansom OJ,Janssen KP,Muschel RJ,Brunner TB

Sun Nov 30 00:00:00 UTC 2014

Loss of PTEN induces microtentacles through PI3K-independent activation of cofilin.

Oncogene

Vitolo MI,Boggs AE,Whipple RA,Yoon JR,Thompson K,Matrone MA,Cho EH,Balzer EM,Martin SS

14-4502 was used in Immunofluorescence to identify a novel mechanism by which PTEN loss could remodel the cortical actin network to facilitate microtentacles.

Thu Apr 25 00:00:00 UTC 2013

Chikungunya virus nsP3 blocks stress granule assembly by recruitment of G3BP into cytoplasmic foci.

Journal of virology

Fros JJ,Domeradzka NE,Baggen J,Geertsema C,Flipse J,Vlak JM,Pijlman GP

14-4502 was used in Immunocytochemistry to reveal a novel role for the Chikungunya virus nonstructural protein nsP3 as a regulator of the cellular stress response.

Mon Oct 01 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: alpha tubulin; alpha-tubulin; Alpha-tubulin 1; Alpha-tubulin 2; Alpha-tubulin 3; Alpha-tubulin 3C; alpha-tubulin 3C/D; Alpha-tubulin 3E; Alpha-tubulin 4; Alpha-tubulin 6; Alpha-tubulin isotype M-alpha-1; Alpha-tubulin isotype M-alpha-2; Alpha-tubulin isotype M-alpha-4; Alpha-tubulin isotype M-alpha-6; Alpha-tubulin ubiquitous; beta-tubulin T beta15 (aa 1-445); class II beta-tubulin isotype; class IIb beta-tubulin; FLJ30169; H2 ALPHA; hum-a-tub1; hum-a-tub2; T beta-15; Talpha1 alpha-tubulin; Testis-specific alpha-tubulin; TUBA1/3/4; Tubulin alpha; tubulin alpha 3; Tubulin alpha chain-like 3; Tubulin alpha-1 chain; Tubulin alpha-1A chain; Tubulin alpha-1B chain; Tubulin alpha-1C chain; Tubulin alpha-2 chain; Tubulin alpha-3 chain; Tubulin alpha-3C chain; Tubulin alpha-3E chain; Tubulin alpha-4 chain; Tubulin alpha-4A chain; Tubulin alpha-6 chain; Tubulin alpha-ubiquitous chain; Tubulin B-alpha-1; Tubulin beta-2B chain; Tubulin H2-alpha; Tubulin K-alpha-1; tubulin, alpha 1; tubulin, alpha 1 (testis specific); tubulin, alpha 1a; tubulin, alpha 1b; tubulin, alpha 1c; tubulin, alpha 2; tubulin, alpha 3c; tubulin, alpha 3e; tubulin, alpha 4; tubulin, alpha 4a; tubulin, alpha 6; tubulin, alpha, brain-specific; tubulin, alpha, ubiquitous; tubulin, alpha-like 3; tubulin, beta 2; tubulin, beta 2B class IIb; tubulin, beta polypeptide paralog

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Gene Aliases: 2410129E14Rik; ALS22; B-ALPHA-1; bA408E5.3; bA506K6.1; bcm948; brdp; H2-ALPHA; K-ALPHA-1; LIS3; M[a]4; M[a]6; PMGYSA; RGD1309427; RGD1565476; Tuba-1; TUBA1; TUBA1A; TUBA1B; TUBA1C; TUBA2; TUBA3; TUBA3C; TUBA3E; Tuba4; TUBA4A; TUBA6; TUBAL3; Tubb2; Tubb2a; TUBB2B

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Function(s)

GTPase activity structural molecule activity structural constituent of cytoskeleton protein binding GTP binding protein domain specific binding double-stranded RNA binding ubiquitin protein ligase binding molecular_function enzyme binding nucleotide binding protein heterodimerization activity

Process(es)

microtubule-based process protein polymerization neuron migration metabolic process biological_process cytoskeleton organization G2/M transition of mitotic cell cycle cytoskeleton-dependent intracellular transport cell division cellular response to interleukin-4 platelet degranulation microtubule cytoskeleton organization

Performance Guarantee

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alpha Tubulin Monoclonal Antibody (DM1A), eBioscience™

alpha Tubulin Antibody (14-4502-82) in ICC/IF

Product Details

14-4502-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

in situ PLA (PLA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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