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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • alpha Tubulin Antibodies

          Invitrogen

          alpha Tubulin Monoclonal Antibody (YL1/2)

          43 Published Figures
          79 References
          View all (82) alpha Tubulin antibodies

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          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite alpha Tubulin Monoclonal Antibody (YL1/2)

          • Antibody Testing Data (3)
          • Published Figures (43)
          alpha Tubulin Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          alpha Tubulin Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 46

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          alpha Tubulin Antibody (MA1-80017) in ICC/IF

          Immunofluorescence analysis of alpha Tubulin was performed using 70% confluent log phase LNCaP cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with alpha Tubulin (YL1/2) Rat Monoclonal Antibody at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temper... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          alpha Tubulin Antibody in Immunocytochemistry (ICC/IF)
          alpha Tubulin Antibody in Western Blot (WB)
          alpha Tubulin Antibody in Western Blot (WB)
          alpha Tubulin Antibody in Western Blot (WB)
          alpha Tubulin Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          alpha Tubulin Antibody in Immunocytochemistry (ICC/IF)
          alpha Tubulin Antibody in Immunocytochemistry (ICC/IF)
          alpha Tubulin Antibody in Immunocytochemistry (ICC/IF)
          alpha Tubulin Antibody in Immunocytochemistry (ICC/IF)
          alpha Tubulin Antibody in Immunocytochemistry (ICC/IF)
          alpha Tubulin Antibody in Immunocytochemistry (ICC/IF)
          alpha Tubulin Antibody in Immunocytochemistry (ICC/IF)
          alpha Tubulin Antibody in Immunocytochemistry (ICC/IF)
          alpha Tubulin Antibody in Immunocytochemistry (ICC/IF)
          alpha Tubulin Antibody in Immunocytochemistry (ICC/IF)
          alpha Tubulin Antibody in Immunocytochemistry (ICC/IF)
          alpha Tubulin Antibody in Immunocytochemistry (ICC/IF)
          alpha Tubulin Antibody in Immunocytochemistry (ICC/IF)
          alpha Tubulin Antibody in Immunocytochemistry (ICC/IF)
          alpha Tubulin Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          alpha Tubulin Antibody in Immunocytochemistry (ICC/IF)
          alpha Tubulin Antibody in Immunocytochemistry (ICC/IF)
          alpha Tubulin Antibody in Immunocytochemistry (ICC/IF)
          alpha Tubulin Antibody in Immunocytochemistry (ICC/IF)
          alpha Tubulin Antibody in Immunocytochemistry (ICC/IF)
          alpha Tubulin Antibody in Immunocytochemistry (ICC/IF)
          alpha Tubulin Antibody in Immunocytochemistry (ICC/IF)
          alpha Tubulin Antibody in Immunocytochemistry (ICC/IF)
          alpha Tubulin Antibody in Immunohistochemistry (IHC)
          alpha Tubulin Antibody in Immunohistochemistry (IHC)
          alpha Tubulin Antibody in Western Blot (WB)
          alpha Tubulin Antibody in Western Blot (WB)
          alpha Tubulin Antibody in Western Blot (WB)
          alpha Tubulin Antibody in Western Blot (WB)
          alpha Tubulin Antibody in Western Blot (WB)
          alpha Tubulin Antibody in Western Blot (WB)
          alpha Tubulin Antibody in Western Blot (WB)
          alpha Tubulin Antibody in Western Blot (WB)
          alpha Tubulin Antibody in Western Blot (WB)
          alpha Tubulin Antibody in Western Blot (WB)
          alpha Tubulin Antibody in Western Blot (WB)
          alpha Tubulin Antibody in Western Blot (WB)
          alpha Tubulin Antibody in Western Blot (WB)
          alpha Tubulin Antibody in Western Blot (WB)
          alpha Tubulin Antibody in Western Blot (WB)
          alpha Tubulin Antibody in Western Blot (WB)

          Product Details

          MA1-80017

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          2 µg/mL
          View 23 publications 23 publications

          Immunohistochemistry (IHC)

          -
          View 14 publications 14 publications

          Immunohistochemistry (PFA fixed) (IHC (PFA))

          -
          View 1 publication 1 publication

          Immunohistochemistry (Frozen) (IHC (F))

          Assay-dependent
          -

          Immunocytochemistry (ICC/IF)

          2 µg/mL
          View 38 publications 38 publications

          Flow Cytometry (Flow)

          -
          View 1 publication 1 publication

          ELISA (ELISA)

          1:100-1:1,000
          -

          Immunoprecipitation (IP)

          Assay-dependent
          -

          Radioimmune Assays (RIA)

          Assay-dependent
          -

          Blocking Assay (BLOCK)

          -
          View 1 publication 1 publication

          Miscellaneous PubMed (MISC)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Dog, Fruit fly, Human, Mouse, Plant, Pig, Rabbit, Rat, Xenopus, Yeast

          Published species

          Arthropod, Bacteria, Cnidarian, Dog, Fungi, Human, Insect, Mouse, Non-human primate, Plant, Protozoa, Rabbit, Rat

          Host/Isotype

          Rat / IgG2a

          Class

          Monoclonal

          Type

          Antibody

          Clone

          YL1/2

          Immunogen

          Yeast tubulin.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Protein G

          Storage buffer

          PBS, pH 7.4

          Contains

          0.09% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2210201

          Product Specific Information

          This clone recognizes the alpha subunit of tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin). The epitope recognized by this antibody appears to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Glu-Glu-Tyr in Tyr-Tubulin).

          MA1-80017 is predicted to react with amphibia, birds, and echinoderm based on sequence homology. Does not react with nephrotoma suturalis.

          This antibody is suitable for use as a loading control.The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin.

          Target Information

          Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: alpha tubulin; alpha tubulin 84B; alpha-Tubulin; Alpha-tubulin 1; Alpha-tubulin 2; Alpha-tubulin 3; Alpha-tubulin 3C; alpha-tubulin 3C/D; Alpha-tubulin 3E; Alpha-tubulin 4; Alpha-tubulin 6; alpha-Tubulin 84B; alpha-Tubulin at 84B; Alpha-tubulin isotype M-alpha-1; Alpha-tubulin isotype M-alpha-2; Alpha-tubulin isotype M-alpha-4; Alpha-tubulin isotype M-alpha-6; Alpha-tubulin ubiquitous; alpha-tubulin84B; alpha1 tubulin; alpha1-tubulin; alpha[[1]] tubulin; alpha[[1]]-tubulin; alphaT; alphaTub84B-PA; alphatubulin; alphaTubulin 84B; alphaTubulin84B; beta-tubulin T beta15 (aa 1-445); CG1913-PA; class II beta-tubulin isotype; class IIb beta-tubulin; FLJ30169; H2 ALPHA; hum-a-tub1; hum-a-tub2; T beta-15; Talpha1 alpha-tubulin; Testis-specific alpha-tubulin; Tub1p; TUBA1/3/4; tubulin; tubulin 1 alpha-chain; tubulin 1alpha; Tubulin alpha; tubulin alpha 3; Tubulin alpha chain-like 3; Tubulin alpha-1 chain; Tubulin alpha-1A chain; Tubulin alpha-1B chain; Tubulin alpha-1C chain; Tubulin alpha-2 chain; Tubulin alpha-3 chain; Tubulin alpha-3C chain; Tubulin alpha-3E chain; Tubulin alpha-4 chain; Tubulin alpha-4A chain; Tubulin alpha-6 chain; Tubulin alpha-ubiquitous chain; tubulin alpha1; tubulin alpha1 promoter; Tubulin B-alpha-1; Tubulin beta-2B chain; Tubulin H2-alpha; Tubulin K-alpha-1; tubulin(alpha1); tubulin, alpha 1; tubulin, alpha 1 (testis specific); tubulin, alpha 1a; tubulin, alpha 1b; tubulin, alpha 1c; tubulin, alpha 2; tubulin, alpha 3c; tubulin, alpha 3e; tubulin, alpha 4; tubulin, alpha 4a; tubulin, alpha 6; tubulin, alpha, brain-specific; tubulin, alpha, ubiquitous; tubulin, alpha-like 3; tubulin, beta 2; tubulin, beta 2B class IIb; tubulin, beta polypeptide paralog; Tubulin-alpha1; tubulin1alpha; tubulin84B

          View more View less

          Gene Aliases: 1t; 2410129E14Rik; a-tub84B; a1t; ALPHA 84C; alpha-Tub; alpha-Tub84B; alpha-tubulin; alpha-tubulin 84B; alpha1; alpha1t; alpha1tub; alpha1tub84B; alpha84B; alphaT; alphat-1; alphaTUB; alphaTub1; alphaTub84; alphaTUB84B; ALS22; anon-EST:Liang-1.59; anon-EST:Liang-2.30; aTub84B; B-ALPHA-1; bA408E5.3; bA506K6.1; bcm948; BEST:LD32507; brdp; CG1913; chr3R:2914276..2914416; clone 1.59; clone 2.30; DM1alpha; Dmel\CG1913; Dmel_CG1913; DTA1; H2-ALPHA; K-ALPHA-1; l(3)84Bd; l(3)g3; LIS3; M[a]4; M[a]6; ms(3)nc33; nc33; PMGYSA; RGD1309427; RGD1565476; T; Talpha1; TBA1_DROME; Tub; TUB1; Tuba-1; TUBA1; TUBA1A; tuba1a-a; tuba1a-b; TUBA1B; TUBA1C; TUBA2; TUBA3; TUBA3C; TUBA3E; Tuba4; TUBA4A; TUBA6; Tuba84B; tubal2; TUBAL3; Tubalpha1; Tubb2; Tubb2a; TUBB2B; tubulin; XELAEV_18019849mg; XELAEV_18020901mg; YML085C

          View more View less

          UniProt ID: (Baker's yeast) P09733, (Human) Q71U36, (Pig) Q2XVP4, (Human) P68363, (Human) Q9BQE3, (Human) P0DPH7, (Human) Q6PEY2, (Human) P68366, (Human) A6NHL2, (Human) Q9BVA1, (Mouse) P68369, (Rat) P68370, (Mouse) P05213, (Rat) Q6P9V9, (Mouse) P68373, (Rat) Q6AYZ1, (Mouse) P68368, (Rat) Q5XIF6, (Mouse) Q3UX10, (Mouse) Q9CWF2, (Rat) Q3KRE8, (Fruit fly) P06603

          View more View less

          Entrez Gene ID: (Rabbit) 100009327, (Rabbit) 100354352, (Dog) 106557476, (Dog) 478702, (Baker's yeast) 854889, (Human) 7846, (Pig) 733594, (Human) 10376, (Human) 84790, (Human) 7278, (Human) 112714, (Dog) 478918, (Human) 7277, (Human) 79861, (Rabbit) 100353478, (Dog) 487148, (Human) 347733, (Pig) 100153507, (Mouse) 22142, (Rat) 64158, (Mouse) 22143, (Rat) 500929, (Mouse) 22146, (Rat) 300218, (Mouse) 22145, (Rat) 316531, (Rat) 291287, (Mouse) 238463, (Mouse) 73710, (Rat) 291081, (Fruit fly) 40848, (Xenopus) 431994, (Xenopus) 100036943

          View more View less

          Function(s)
          nucleotide binding GTPase activity structural constituent of cytoskeleton GTP binding structural molecule activity protein binding protein domain specific binding double-stranded RNA binding ubiquitin protein ligase binding molecular_function enzyme binding protein heterodimerization activity
          Process(es)
          microtubule-based process protein polymerization neuron migration metabolic process biological_process cytoskeleton organization G2/M transition of mitotic cell cycle cytoskeleton-dependent intracellular transport cell division cellular response to interleukin-4 mitotic sister chromatid segregation nuclear migration involved in conjugation with cellular fusion obsolete GTP catabolic process nuclear migration along microtubule homologous chromosome segregation platelet degranulation microtubule cytoskeleton organization mitotic spindle organization mitotic spindle assembly checkpoint antimicrobial humoral response
          It has to be done as per old AB suggested Products section.
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