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Invitrogen
alpha/beta Tubulin Monoclonal Antibody (TU-10)
FIGURE: 1 / 4
alpha/beta Tubulin Antibody (MA1-19400) in ICC/IF
Immunofluorescence analysis of alpha/beta Tubulin was performed using 70% confluent log phase LNCaP cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with alpha/beta Tubulin (TU-10) Mouse Monoclonal Antibody (Product # MA1-19400) at 2µg/mL in 0.1% BSA and in... View More
Product Details
MA1-19400
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Many,
Mouse,
Pig,
Rat
Published species
Human
Host/Isotype
Mouse
/ IgM
Class
Monoclonal
Type
Antibody
Clone
TU-10
Immunogen
Microtubule proteins from porcine brain.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
purified
Storage buffer
TBS, pH 8
Contains
15mM sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1070282
Product Specific Information
This antibody recognizes alpha, beta-tubulin heterodimer, a basic intracellular structural unit of microtubules. Alpha- and beta-tubulins form approximately 100 kDa tubulin heterodimer, a globular protein that polymerizes to form microtubules.
Western Blot: Reducing conditions.
Target Information
Tubulin protein is the major constituent of microtubules. Tubulin is a dimer of alpha and beta chains, which binds two molecules of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. There are at least six isotypes of both alpha- and beta- tubulin in human cells, which are distinguished by slightly different amino acid sequences and encoded by a large, multigene family that has been highly conserved throughout evolution. Although the most important functions of microtubules in proliferative cells are through their actions as components of the mitotic spindle, they are also involved in many other essential functions throughout the cell cycle of both malignant and nonmalignant cells. Anti-microtubule agents including Vinca alkaloids and taxanes may disrupt many of these essential functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Alpha-tubulin 1; Alpha-tubulin 3; Alpha-tubulin isotype M-alpha-1; beta 5-tubulin; beta Ib tubulin; FLJ30169; H2 ALPHA; hum-a-tub1; hum-a-tub2; Talpha1 alpha-tubulin; Tubulin alpha; Tubulin alpha-1 chain; Tubulin alpha-1A chain; Tubulin alpha-3 chain; Tubulin B-alpha-1; tubulin beta; Tubulin beta chain; Tubulin beta-1 chain; Tubulin beta-5 chain; tubulin, alpha 1; tubulin, alpha 1a; tubulin, alpha, brain-specific; tubulin, beta 1 class VI; tubulin, beta class I; tubulin, beta family 1; tubulin, beta polypeptide; α/β-tubulin
Gene Aliases: 2810484G07Rik; B-ALPHA-1; CDCBM6; CH242-247L10.4; CSCSC1; LIS3; M(beta)1; M40; OK/SW-cl.56; Tuba-1; Tuba1; TUBA1A; TUBA3; TUBB; TUBB1; TUBB5
UniProt ID: (Human) Q71U36, (Human) P07437, (Human) Q9H4B7, (Pig) Q767L7, (Mouse) P68369, (Rat) P68370, (Mouse) A2AQ07
Entrez Gene ID: (Human) 7846, (Human) 203068, (Pig) 100134983, (Human) 81027, (Pig) 733686, (Mouse) 22142, (Rat) 64158, (Mouse) 545486, (Rat) 679312
GTPase activity
structural molecule activity
structural constituent of cytoskeleton
protein binding
GTP binding
protein domain specific binding
ubiquitin protein ligase binding
protein complex binding
GTPase activating protein binding
MHC class I protein binding
nucleotide binding
protein heterodimerization activity
microtubule-based process
protein polymerization
spindle assembly
G2/M transition of mitotic cell cycle
movement of cell or subcellular component
cellular process
cytoskeleton-dependent intracellular transport
natural killer cell mediated cytotoxicity
cell division
metabolic process
cytoskeleton organization
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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