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Invitrogen
beta-1,2 Adaptin Monoclonal Antibody (100/1)
Product Details
MA1-25065
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Rat
Published species
Bovine
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
100/1
Immunogen
AP-2 adaptor protein from bovine brain.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
Ascites
Contains
0.1% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2058203
Product Specific Information
Recommended positive controls: rat brain, MDCK.
This antibody stains clathrin-coated membranes at the plasma membrane, and in the Golgi region of methanol-fixed MDBK cells. By Western bloy on bovine and rat brain preparations, this antibody detects a major band of AP-1 at ~115 kDa and a minor component at ~110 kDa, and bands at ~104 and ~112 kDa representing AP2. The antibody reacts with polypeptides at ~100 kDa in bovine liver, human heart fibroblasts and MDBK cells, but not with any components in the 110-115 kDa range from these sources, or from rat PC12 cells, neuroblastoma or astrocytes.
Target Information
Adaptor protein complex 1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors. This complex is a heterotetramer composed of two large, one medium, and one small adaptin subunit. The protein encoded by this gene serves as one of the large subunits of this complex and is a member of the adaptin protein family. This gene is a candidate meningioma gene. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: adapter-related protein complex 1 subunit beta-1; adapter-related protein complex 2 beta subunit; adapter-related protein complex 2 subunit beta; adaptin, beta 2 (beta); Adaptor protein complex AP-1 subunit beta-1; adaptor protein complex AP-1, beta 1 subunit; Adaptor protein complex AP-2 subunit beta; adaptor related protein complex 2, beta 1 subunit; adaptor-related protein complex 1 beta 1 subunit; Adaptor-related protein complex 1 subunit beta-1; adaptor-related protein complex 1, beta 1 subunit; Adaptor-related protein complex 2 subunit beta; adaptor-related protein complex 2, beta 1 subunit; adaptor-related protein complex AP-1 beta 1 subunit; AP-1 complex subunit beta-1; AP-2 complex subunit beta; AP105B; beta adaptin; Beta-1-adaptin; Beta-2-adaptin; Beta-adaptin; Beta-adaptin 1; beta-prime-adaptin; beta1-adaptin; beta2-adaptin; Clathrin assembly protein complex 1 beta large chain; Clathrin assembly protein complex 2 beta large chain; clathrin-associated / assembly / adaptor protein, large, beta 1; clathrin-associated/assembly/adaptor protein, large, beta 1; Golgi adaptor HA1/AP1 adaptin beta subunit; Plasma membrane adaptor HA2/AP2 adaptin beta subunit; plasma membrane adaptor HA2/AP2 adaptor beta subunit; testicular tissue protein Li 22
Gene Aliases: ADTB1; ADTB2; AP105A; AP105B; AP1B1; AP2-BETA; AP2B1; BAM22; BOS_16712; BOS_18468; CLAPB1; CLAPB2
UniProt ID: (Human) Q10567, (Bovine) Q08DS7, (Human) P63010, (Rat) P62944
Entrez Gene ID: (Bovine) 506192, (Human) 162, (Bovine) 282183, (Human) 163, (Rat) 29663, (Rat) 140670
intracellular protein transport
vesicle-mediated transport
clathrin coat assembly
endocytosis
determination of left/right symmetry
heart development
antigen processing and presentation of exogenous peptide antigen via MHC class II
regulation of defense response to virus by virus
ventricular septum development
microtubule-based movement
aorta development
negative regulation of epidermal growth factor receptor signaling pathway
ephrin receptor signaling pathway
Wnt signaling pathway, planar cell polarity pathway
coronary vasculature development
clathrin-mediated endocytosis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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