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Rockland
beta 1 Adrenergic Receptor Polyclonal Antibody
FIGURE: 1 / 2
beta 1 Adrenergic Receptor Antibody (600-401-MH1) in WB
Western Blot of Rabbit Anti-Beta 1 Adrenergic Receptor Antibody. Lane 1: Opal Pre-Stained Molecular Weight Marker (p/n MB-210-0500). Lane 2: Human heart muscle membrane tissue lysate (35ug) [+]. Lane 3: K562 whole cell lysate (35ug) [-]. Primary Antibody: Anti-Beta 1 Adrenergic Receptor at 1:1000 overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit HRP (p/n 611-103-122) at 1:5000 for 30 ins at RT. ... View More
Product Details
600-401-MH1
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-BETA 1 ADRENERGIC RECEPTOR (RABBIT) Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human beta 1 adrenergic conjugated to Keyhole Limpet Hemocyanin (KLH).
Conjugate
Form
Liquid
Concentration
0.97 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against human beta 1 adrenergic. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to human, mouse, rat, and rhesus macaque.
Target Information
Adrenergic receptors (ARs) are members of the 7-transmembrane domain G-protein-coupled receptor superfamily that bind the endogenous catecholamines epinephrine and norepinephrine. Pharmacological, structural, and molecular cloning data indicate significant heterogeneity within this receptor family. Nine receptor subtypes have been identified thus far including three alpha-1 AR subtypes (1A/D, 1B, and 1C), three alpha-2 ARs (2A, 2B, and 2C), and three beta AR subtypes (1, 2, and 3). ARs participate in either the onset or maintenance of several disease states including hypertension, cardiac dysfunction (congestive heart failure, ischemia, arrhythmias), diabetes, glaucoma, depression, and impotence. BARs participate in diverse processes including development, behavior, cardiac function, smooth muscle tone, and metabolism. In gene-knockout experiments, the majority of mice that lack the B1AR gene die prenatally and those that do survive until adulthood display abnormal cardiac function. Other studies have shown that the direct regulation of cardiac B1AR density by thyroid hormones occurs at the transcriptional level and is modulated by the catecholamine sensitive-adenylyl cyclase system. Evidence for the effects of cAMP on B1AR regulation has come from a study of members of the cAMP response element (CRE) modulator (CREM) family of transcription factors in transformed cell lines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: adrenergic, beta-1-, receptor; Beta-1 adrenergic receptor; Beta-1 adrenoceptor; Beta-1 adrenoreceptor
Gene Aliases: ADRB1; ADRB1R; B1AR; BETA1AR; RHR
UniProt ID: (Human) P08588
Entrez Gene ID: (Human) 153
beta-adrenergic receptor activity
beta1-adrenergic receptor activity
receptor signaling protein activity
Ras guanyl-nucleotide exchange factor activity
protein binding
PDZ domain binding
alpha-2A adrenergic receptor binding
protein heterodimerization activity
epinephrine binding
norepinephrine binding
temperature homeostasis
positive regulation of heart rate by epinephrine-norepinephrine
positive regulation of the force of heart contraction by epinephrine-norepinephrine
diet induced thermogenesis
vasodilation by norepinephrine-epinephrine involved in regulation of systemic arterial blood pressure
activation of adenylate cyclase activity
cell-cell signaling
response to cold
positive regulation of cAMP biosynthetic process
heat generation
negative regulation of multicellular organism growth
fear response
positive regulation of GTPase activity
positive regulation of cAMP-mediated signaling
brown fat cell differentiation
adenylate cyclase-activating adrenergic receptor signaling pathway
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