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beta-2 Adaptin Antibody (PA1-1066) in ICC/IF
Immunofluorescent analysis of beta-2 Adaptin was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with beta-2 Adaptin Rabbit Polyclonal Antibody (Product # PA1-1066) at 2 µg/mL in 0.1% BSA and incubated for 3 ... View More
Product Details
PA1-1066
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues I(588) H R K H L P I H H G S T D A G D S P(606) of human beta Adaptin 2.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2056349
Product Specific Information
PA1-1066 detects beta Adaptin 2 from human, mouse, rat, and bovine samples.
PA1-1066 has been successfully used in Western blot procedures.
The PA1-1066 immunogen is a synthetic peptide corresponding to residues I(588) H R K H L P I H H G S T D A G D S P(606) of human beta Adaptin 2.
PA1-1066 can be used with blocking peptide PEP-293.
Target Information
Clathrin-mediated endocytosis is the pathway by which many receptors for nutrients and hormones are internalized to be recycled or down-regulated. During formation of clathrin coated membranes, clathrin co-assembles with heterotetrameric molecules known as assembly polypeptides (APs) or adaptors which form a layer of protein coat between the clathrin lattice and the membrane. There are two characterized adaptors AP1 and AP2. AP1 is associated with clathrin coated vesicles at the trans-Golgi network and AP2 is associated with the endocytic clathrin coated vesicles at the plasma membrane and has been shown to specifically interact with Shc and EGF receptor. AP2 is composed of four subunits, two separate ~100 kDa gene products with similar domain structures (alpha and beta adaptin) and a ~50 and ~17 kDa subunit. There are two alpha-adaptin genes, alpha A and alpha C which have a tissue specific pattern of expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: adapter-related protein complex 1 subunit beta-1; adapter-related protein complex 2 beta subunit; adapter-related protein complex 2 subunit beta; adaptin, beta 2 (beta); Adaptor protein complex AP-1 subunit beta-1; Adaptor protein complex AP-2 subunit beta; adaptor related protein complex 2, beta 1 subunit; Adaptor-related protein complex 1 subunit beta-1; Adaptor-related protein complex 2 subunit beta; adaptor-related protein complex 2, beta 1 subunit; AP-1 complex subunit beta-1; AP-2 complex subunit beta; AP105B; beta adaptin; Beta-1-adaptin; Beta-2-adaptin; Beta-adaptin; Beta-adaptin 1; beta1-adaptin; beta2-adaptin; Clathrin assembly protein complex 1 beta large chain; Clathrin assembly protein complex 2 beta large chain; clathrin-associated / assembly / adaptor protein, large, beta 1; clathrin-associated/assembly/adaptor; clathrin-associated/assembly/adaptor protein, large, beta 1; Golgi adaptor HA1/AP1 adaptin beta subunit; Plasma membrane adaptor HA2/AP2 adaptin beta subunit; testicular tissue protein Li 22
Gene Aliases: 1300012O03Rik; ADTB2; AI788979; AP105B; AP2-BETA; AP2B1; BOS_18468; CLAPB1
UniProt ID: (Bovine) Q08DS7, (Human) P63010, (Rat) P62944, (Mouse) Q9DBG3
Entrez Gene ID: (Bovine) 282183, (Human) 163, (Rat) 140670, (Mouse) 71770
intracellular protein transport
endocytosis
clathrin coat assembly
transport
protein transport
vesicle-mediated transport
ventricular septum development
microtubule-based movement
antigen processing and presentation of exogenous peptide antigen via MHC class II
aorta development
negative regulation of epidermal growth factor receptor signaling pathway
ephrin receptor signaling pathway
regulation of defense response to virus by virus
Wnt signaling pathway, planar cell polarity pathway
coronary vasculature development
clathrin-mediated endocytosis
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