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beta-2 Defensin Antibody (500-P161G-1MG) in WB
Western Blot: To detect Human BD-2 by Western Blot analysis beta-2 Defensin Polyclonal Antibody (Product # 500-P161G-1MG) can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human BD-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P161G-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Goat
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human BD-2
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929557
Product Specific Information
AA Sequence of recombinant protein: GIGDPVTCLK SGAICHPVFC PRRYKQIGTC GLPGTKCCKK P.
Preparation: Produced from sera of goats immunized with highly pure Recombinant Human BD-2. Anti-Human BD-2-specific antibody was purified by affinity chromatography employing an immobilized Human BD-2 matrix.
Sandwich ELISA: To detect Human BD-2 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human BD-2 (500-P161GBt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human BD-2.
Western Blot: To detect Human BD-2 by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human BD-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The alpha-defensins are distinguished from the beta-defensins by the pairing of their three disulfide bonds. To date, six human beta-defensins have been identified; BD-1, BD-2, BD-3, BD-4, BD-5 and BD-6. beta-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they can act as chemoattractants towards immature dendritic cells and memory T cells. The beta-defensin proteins are expressed as the C-terminal portion of precursors, and are released by proteolytic cleavage of a signal sequence and, in some cases, a propeptide sequence. Beta-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: BD-2; beta defensin 2; beta defensin-2; Beta-defensin 2; Defensin beta 2; Defensin beta 4A; Defensin beta2; Defensin, beta 2; defensin, beta 4; defensin, beta 4A; Defensin-beta2; SAP1; Skin-antimicrobial peptide 1
Gene Aliases: BD-2; DEFB-2; DEFB102; DEFB2; DEFB4; DEFB4A; DEFB4B; HBD-2; SAP1
UniProt ID: (Human) O15263
Entrez Gene ID: (Human) 1673
Performance Guarantee
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