Invitrogen

beta-2 Microglobulin Recombinant Rabbit Monoclonal Antibody (4H5L6)

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1 Published Figure
2 References
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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

701250

Applications
Tested Dilution
Publications

Western Blot (WB)

1-2 µg/mL
View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20
-

Immunocytochemistry (ICC/IF)

1:100
-
Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

4H5L6

Immunogen

Recombinant protein corresponding to amino acids 21-119 of human b2-microglobulin
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532441

Product Specific Information

This antibody is predicted to react with non-human primate, mouse and rat based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Beta-2-microglobulin (B2M) is a 12-14 kDa protein that is a subunit of the major histocompatibility complex (MHC) class I. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells, but is absent on erythrocytes. Beta-2 microglobulin is also found in free form in the plasma. The amino acid sequence of Beta-2-microglobulin is homologous to the constant regions of immunoglobulin heavy and light chains, with a predominantly beta pleated sheet structure. The protein is ubiquitously expressed in the cytoplasm, and is involved in antigen processing and presentation. Beta-2-microglobulin also plays a role in TCR signaling, and downstream signaling in naive CD8+ cells. Beta 2-microglobulin is encoded by the B2M gene located on chromosome 15. A mutation in the Beta-2-microglobulin gene has been shown to result in hypercatabolic hypoproteinemia. Levels of beta 2 microglobulin can be used to assess renal function in kidney transplant recipients. Beta-2-microglobulin that protein can be used as a prognostic marker for HIV infection.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: B-F; B2-microglobulin; Beta 2 microglobin; Beta 2 microglobulin; beta chain of MHC class I molecules; beta-2-microglobin; Beta-2-microglobulin; Beta2 microglobulin; MHC Class I; microglobulin beta-2; Orla-B2m

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Gene Aliases: B2M; beta2-m; beta2m; CDABP0092; HDCMA22P; IMD43; Ly-m11

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UniProt ID: (Human) P61769, (Mouse) P01887

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Entrez Gene ID: (Human) 567, (Mouse) 12010

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Function(s)
glycoprotein binding protein binding identical protein binding
Process(es)
retina homeostasis positive regulation of T cell mediated cytotoxicity antigen processing and presentation of peptide antigen via MHC class I antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent positive regulation of T cell cytokine production regulation of membrane depolarization negative regulation of neuron projection development antibacterial humoral response antigen processing and presentation of endogenous peptide antigen via MHC class I positive regulation of protein binding T cell differentiation in thymus protein refolding response to drug cellular protein metabolic process innate immune response response to cadmium ion positive regulation of receptor-mediated endocytosis regulation of defense response to virus by virus regulation of immune response defense response to Gram-negative bacterium defense response to Gram-positive bacterium iron ion homeostasis interferon-gamma-mediated signaling pathway cellular response to lipopolysaccharide cellular response to iron ion negative regulation of receptor binding positive regulation of receptor binding positive regulation of ferrous iron import into cell positive regulation of ferrous iron binding positive regulation of transferrin receptor binding response to molecule of bacterial origin immune system process cellular defense response
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