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Invitrogen
beta-3 Adrenergic Receptor Polyclonal Antibody
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FIGURE: 1 / 4
beta-3 Adrenergic Receptor Antibody (PA5-143874) in ICC/IF
Immunocytochemistry analysis of beta-3 Adrenergic Receptor in U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in beta-3 Adrenergic Receptor Polyclonal antibody (Product # PA5-143874) using a dilution of 2 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary a... View More
Product Details
PA5-143874
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human beta 3 Adrenergic Receptor/ADRB3 recombinant protein (Position: R179-S250).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3075088
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human HEK293 whole cell, rat liver tissue, mouse liver tissue. ICC/IF: U20S cell .|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
beta 3 Adrenergic Receptor is located mainly in adipose tissue and is involved in the regulation of lipolysis and thermogenesis. Beta adrenergic receptors are involved in the epinephrine and norepinephrine-induced activation of adenylate cyclase through the action of G proteins. beta 3 Adrenergic Receptor expression has been documented in adipose tissues, heart, and in smooth muscle of digestive and urinary tract organs (bladder, colon, small intestine, stomach, ureter). Utilization of alternate promoters and/or 3-prime untranslated regions may result in tissue-specific regulation of the expression of this protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: adrenergic receptor, beta 3; adrenergic, beta-3-, receptor; beta 3 adrenoceptor; beta 3-AR; Beta-3 adrenergic receptor; Beta-3 adrenoceptor; Beta-3 adrenoreceptor; Beta-3 AR; beta3-adrenergic receptor; beta3-Adrenoreceptor; Beta3-AR; FLJ99960
Gene Aliases: ADRB; Adrb-3; ADRB3; ADRB3R; B3AR; B3bar; BETA3AR
UniProt ID: (Human) P13945, (Mouse) P25962, (Rat) P26255
Entrez Gene ID: (Human) 155, (Mouse) 11556, (Rat) 25645
temperature homeostasis
diet induced thermogenesis
vasodilation by norepinephrine-epinephrine involved in regulation of systemic arterial blood pressure
desensitization of G-protein coupled receptor protein signaling pathway by arrestin
carbohydrate metabolic process
generation of precursor metabolites and energy
energy reserve metabolic process
receptor-mediated endocytosis
G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger
adenylate cyclase-modulating G-protein coupled receptor signaling pathway
activation of adenylate cyclase activity
cell-cell signaling
aging
response to cold
heat generation
negative regulation of multicellular organism growth
eating behavior
positive regulation of MAPK cascade
response to antibiotic
brown fat cell differentiation
adenylate cyclase-activating adrenergic receptor signaling pathway
signal transduction
G-protein coupled receptor signaling pathway
adenylate cyclase-activating G-protein coupled receptor signaling pathway
adrenergic receptor signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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