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beta-3 Defensin Antibody (500-P241BT-25UG) in ELISA
Sandwich ELISA: To detect hBD-3 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of beta-3 Defensin Polyclonal Antibody, Biotin (Product # 500-P241BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech beta-3 Defensin Polyclonal Antibody (Product # 500-P241-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hBD-3.
Product Details
500-P241BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human BD-3
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930004
Product Specific Information
AA Sequence of recombinant protein: GIINTLQKYY CRVRGGRCAV LSCLPKEEQI GKCSTRGRKC CRRKK.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human BD-3. Anti-Human BD-3-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hBD-3 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human BD-3 (500-P241) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hBD-3.
Western Blot: To detect hBD-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hBD-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P241BT-1MG will be provided as 2 x 500 µg
Target Information
Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 103, which has broad spectrum antimicrobial activity and may play an important role in innate epithelial defense. [provided by RefSeq, Oct 2014].
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: BD-3; beta defensin 3; beta defensin-3; Beta-defensin 103; Beta-defensin 3; Defensin, beta 103; defensin, beta 103A; defensin, beta 3; Defensin-like protein
Gene Aliases: BD-3; BD3; DEFB-3; DEFB103; DEFB103A; DEFB103B; DEFB3; hBD-3; HBD3; HBP-3; HBP3
UniProt ID: (Human) P81534
Entrez Gene ID: (Human) 414325
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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