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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Beta III Tubulin Antibodies

          Invitrogen

          beta-3 Tubulin Polyclonal Antibody

          Advanced Verification
          2 Published Figures
          7 References
          View all (51) Beta III Tubulin antibodies

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          Datasheet
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          Datasheet
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          Cite beta-3 Tubulin Polyclonal Antibody

          • Antibody Testing Data (15)
          • Published Figures (2)
          • Advanced Verification (4)
          beta-3 Tubulin Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          beta-3 Tubulin Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 21

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          beta-3 Tubulin Antibody (PA5-85639) in ICC/IF

          beta-3 Tubulin Polyclonal Antibody detects beta Tubulin 3/ Tuj1 protein at cytoplasm by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: beta Tubulin 3/ Tuj1 protein stained by beta-3 Tubulin Polyclonal Antibody (Product # PA5-85639) diluted at 1:500. Blue: Fluoroshield with DAPI . {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          beta-3 Tubulin Antibody in Immunocytochemistry (ICC/IF)
          beta-3 Tubulin Antibody in Immunocytochemistry (ICC/IF)
          beta-3 Tubulin Antibody in Immunocytochemistry (ICC/IF)
          beta-3 Tubulin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          beta-3 Tubulin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          beta-3 Tubulin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          beta-3 Tubulin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          beta-3 Tubulin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          beta-3 Tubulin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          beta-3 Tubulin Antibody in Immunohistochemistry (Frozen) (IHC (F))
          beta-3 Tubulin Antibody in Western Blot (WB)
          beta-3 Tubulin Antibody in Western Blot (WB)
          beta-3 Tubulin Antibody in Western Blot (WB)
          beta-3 Tubulin Antibody in Western Blot (WB)
          beta-3 Tubulin Antibody in Immunoprecipitation (IP)
          beta-3 Tubulin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          beta-3 Tubulin Antibody in Immunocytochemistry (ICC/IF)
          beta-3 Tubulin Antibody
          beta-3 Tubulin Antibody
          beta-3 Tubulin Antibody
          beta-3 Tubulin Antibody

          Product Details

          PA5-85639

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:50,000
          View 1 publication 1 publication

          Immunohistochemistry (IHC)

          -
          View 2 publications 2 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:100-1:1,000
          View 1 publication 1 publication

          Immunohistochemistry (Frozen) (IHC (F))

          1:100-1:1,000
          -

          Immunocytochemistry (ICC/IF)

          1:100-1:1,000
          View 2 publications 2 publications

          Flow Cytometry (Flow)

          Assay-dependent
          -

          Immunoprecipitation (IP)

          1:100-1:500
          -

          Miscellaneous PubMed (MISC)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human beta Tubulin 3/ TUJ1. The exact sequence is proprietary.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.1 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7, with 20% glycerol

          Contains

          0.025% ProClin 300

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2792779

          Product Specific Information

          Keep as concentrated solution.

          Positive Control: HeLa, U87-MG, SK-N-SH, IMR32, SK-N-AS, mouse brain, rat brain.

          Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

          Target Information

          The betaIII-tubulin isoform is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The betaIII-tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: beta 3 tubulin; beta tubulin 3; betaIII-tubulin; class III beta-tubulin; MC1R; Neuron specific beta III Tubulin; Neuron-specific class III beta-tubulin; TUBB; tubulin beta; Tubulin beta-3; Tubulin beta-3 chain; Tubulin beta-4; Tubulin beta-4 chain; Tubulin beta-III; tubulin, beta 3 class III

          View more View less

          Gene Aliases: 3200002H15Rik; beta-4; CDCBM; CDCBM1; CFEOM3; CFEOM3A; FEOM3; M(beta)3; M(beta)6; TUBB3; TUBB4

          View more View less

          UniProt ID: (Human) Q13509, (Mouse) Q9ERD7, (Rat) Q4QRB4

          View more View less

          Entrez Gene ID: (Human) 10381, (Mouse) 22152, (Rat) 246118

          View more View less

          Function(s)
          GTPase activity structural constituent of cytoskeleton protein binding GTP binding peptide binding nucleotide binding
          Process(es)
          microtubule-based process mitotic nuclear division axon guidance neuron differentiation protein polymerization metabolic process cytoskeleton organization
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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