Invitrogen

beta-3 Tubulin Monoclonal Antibody (TU-20)

beta-3 Tubulin Antibody in Immunocytochemistry (ICC/IF)

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beta-3 Tubulin Antibody (MA1-19187) in ICC/IF

Immunocytochemistry staining of Neuro2a mouse neuroblastoma cell line using anti-betaIII-tubulin (TU-20; green; 3 µg/mL) Monoclonal antibody (Product # MA1-19187). Nuclei were stained with DAPI (blue).

beta-3 Tubulin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

beta-3 Tubulin Antibody in Western Blot (WB)

beta-3 Tubulin Antibody in Flow Cytometry (Flow)

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Product Details

MA1-19187

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL, 90 min

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

10 µg/mL

Immunocytochemistry (ICC/IF)

Assay-dependent

View 4 publications 4 publications

Flow Cytometry (Flow)

1-4 µg/mL

Product Specifications

Species Reactivity

Human, Many, Mouse, Rat

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

TU-20

Immunogen

Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

4°C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_1070721

Product Specific Information

This antibody recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) of neuron-specific human betaIII-tubulin.

For immunohistochemistry applications, this product requires protein pretreatment (0.1% trypsin) in 0.1 M HCl; incubate for 30 minutes at room temperature.

Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.

Western Blot: Reducing conditions.

Target Information

The betaIII-tubulin isoform is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The betaIII-tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Genomic, transcriptomic, and metabolomic profiles of hiPSC-derived dopamine neurons from clinically discordant brothers with identical PRKN deletions.

NPJ Parkinson's disease

Cukier HN,Kim H,Griswold AJ,Codreanu SG,Prince LM,Sherrod SD,McLean JA,Dykxhoorn DM,Ess KC,Hedera P,Bowman AB,Neely MD

MA1-19187 was used in Immunocytochemistry-immunoflourescence to identify genetic differences which could potentially, at least partially, contribute to the discordant clinical PD presentation of the two brothers.

Wed Jun 29 00:00:00 UTC 2022

Stingray Venom Proteins: Mechanisms of Action Revealed Using a Novel Network Pharmacology Approach.

Marine drugs

Kirchhoff KN,Billion A,Voolstra CR,Kremb S,Wilke T,Vilcinskas A

MA1-19187 was used in Immunocytochemistry to develop a novel network pharmacology approach based on multi-omics functional data integration to predict how stingray venom disrupts the physiological systems of target animals.

Fri Dec 24 00:00:00 UTC 2021

Single cell RNA sequencing detects persistent cell type- and methylmercury exposure paradigm-specific effects in a human cortical neurodevelopmental model.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Diana Neely M,Xie S,Prince LM,Kim H,Tukker AM,Aschner M,Thimmapuram J,Bowman AB

MA1-19187 was used in Immunocytochemistry-immunofluorescence to demonstrate that MeHg results in changes in gene expression in human developing cortical neurons that manifest well after cessation of exposure and that these changes are cell type-, developmental stage-, and exposure paradigm-specific.

Sun Aug 01 00:00:00 UTC 2021

From the Cover: Manganese and Rotenone-Induced Oxidative Stress Signatures Differ in iPSC-Derived Human Dopamine Neurons.

Toxicological sciences : an official journal of the Society of Toxicology

Neely MD,Davison CA,Aschner M,Bowman AB

MA1-19187 was used in Immunocytochemistry-immunoflourescence to suggest that the cellular oxidative stress evoked by these 2 agents is distinct yielding unique oxidative stress signatures across outcome measures.

Sun Oct 01 00:00:00 UTC 2017

Progerin impairs chromosome maintenance by depleting CENP-F from metaphase kinetochores in Hutchinson-Gilford progeria fibroblasts.

Oncotarget

Eisch V,Lu X,Gabriel D,Djabali K

MA1-19187 was used in western blot test if progerin elicits spatiotemporal deviations in mitotic processes in Hutchinson-Gilford progeria syndrome fibroblasts

Tue Apr 26 00:00:00 UTC 2016

Novel crystalloid oligodendrogliopathy in hereditary spastic paraplegia.

Acta neuropathologica

Woehrer A,Laszlo L,Finsterer J,Stöllberger C,Furtner J,Rinner W,Molnar K,Budka H,Kovacs GG

MA1-19187 was used in immunohistochemistry to study crystalloid oligodendrogliopathy in hereditary spastic paraplegia

Mon Oct 01 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: beta 3 tubulin; beta tubulin 3; betaIII-tubulin; class III beta-tubulin; MC1R; Neuron specific beta III Tubulin; Neuron-specific class III beta-tubulin; TUBB; tubulin beta; Tubulin beta-3; Tubulin beta-3 chain; Tubulin beta-4; Tubulin beta-4 chain; Tubulin beta-III; tubulin, beta 3 class III

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Gene Aliases: 3200002H15Rik; beta-4; CDCBM; CDCBM1; CFEOM3; CFEOM3A; FEOM3; M(beta)3; M(beta)6; TUBB3; TUBB4

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UniProt ID: (Human) Q13509, (Mouse) Q9ERD7, (Rat) Q4QRB4

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Entrez Gene ID: (Human) 10381, (Mouse) 22152, (Rat) 246118

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Function(s)

GTPase activity structural constituent of cytoskeleton protein binding GTP binding peptide binding nucleotide binding

Process(es)

microtubule-based process mitotic nuclear division axon guidance neuron differentiation protein polymerization metabolic process cytoskeleton organization

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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beta-3 Tubulin Monoclonal Antibody (TU-20)

beta-3 Tubulin Antibody (MA1-19187) in ICC/IF

Product Details

MA1-19187

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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