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beta-5 Defensin Antibody (500-P323-1MG) in WB
Western Blot: To detect hBD-5 by Western Blot analysis beta-5 Defensin Polyclonal Antibody (Product # 500-P323-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hBD-5 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P323-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human BD-5
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930410
Product Specific Information
AA Sequence of recombinant protein: GLDFSQPFPS GEFAVCESCK LGRGKCRKEC LENEKPDGNC RLNFLCCRQR I.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human BD-5. Anti-Human BD-5-specific antibody was purified by affinity chromatography employing an immobilized Human BD-5 matrix.
Sandwich ELISA: To detect Human BD-5 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human BD-5 (500-P323Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human BD-5.
Western Blot: To detect hBD-5 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hBD-5 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The alpha-defensins are distinguished from the beta-defensins by the pairing of their three disulfide bonds. To date, six human beta-defensins have been identified; BD-1, BD-2, BD-3, BD-4, BD-5 and BD-6. beta-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they can act as chemoattractants towards immature dendritic cells and memory T cells. The beta-defensin proteins are expressed as the C-terminal portion of precursors, and are released by proteolytic cleavage of a signal sequence and, in some cases, a propeptide sequence. Beta-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BD-5; beta defensin 5; Beta-defensin 105; Beta-defensin 5; Defensin, beta 105; defensin, beta 105A; defensin, beta 105B; defensin, beta 5
Gene Aliases: BD-5; BD5; DEFB-5; DEFB105; DEFB105A; DEFB105B; DEFB5
UniProt ID: (Human) Q8NG35
Entrez Gene ID: (Human) 245908, (Human) 504180
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