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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • beta Actin Antibodies

          Invitrogen

          beta Actin Polyclonal Antibody

          32 Published Figures
          46 References
          View all (92) beta Actin antibodies

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          Datasheet
          Protocols
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          Datasheet
          Protocols
          Questions & Answers

          Cite beta Actin Polyclonal Antibody

          • Antibody Testing Data (9)
          • Published Figures (32)
          beta Actin Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          beta Actin Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 41

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          beta Actin Antibody (PA1-16889) in ICC/IF

          Immunofluorescence analysis of beta Actin was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with beta Actin Polyclonal Antibody (Product # PA1-16889) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          beta Actin Antibody in Immunocytochemistry (ICC/IF)
          beta Actin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          beta Actin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)

          Product Details

          PA1-16889

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:2,000-1:10,000
          View 42 publications 42 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:2000-1:10000
          -

          Immunocytochemistry (ICC/IF)

          5 µg/mL
          -

          Immunoprecipitation (IP)

          Assay-Dependent
          View 2 publications 2 publications

          ChIP assay (ChIP)

          -
          View 1 publication 1 publication

          Dot blot (DB)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Dog, Human, Mouse, Non-human primate

          Published species

          Arthropod, Bison, Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          N-terminus of human beta actin using the numbering given in Swiss-prot entry P60709 and is highly conserved with gamma actin
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7-8

          Contains

          0.05% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_568434

          Product Specific Information

          Suggested positive control: Hela whole cell extract.

          Target Information

          Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: actin, alpha 1, skeletal muscle; actin, beta; Actin, cytoplasmic 1; beta cytoskeletal actin; Beta-actin; PS1TP5-binding protein 1

          View more View less

          Gene Aliases: ACTA1; ACTB; Actx; beta-actin; BRWS1; E430023M04Rik; PS1TP5BP1

          View more View less

          UniProt ID: (Human) P60709, (Dog) O18840, (Mouse) P60710

          View more View less

          Entrez Gene ID: (Human) 60, (Dog) 403580, (Mouse) 11461

          View more View less

          Function(s)
          RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding structural constituent of cytoskeleton protein binding ATP binding kinesin binding Tat protein binding nucleosomal DNA binding identical protein binding nitric-oxide synthase binding
          Process(es)
          retina homeostasis movement of cell or subcellular component cytoskeleton organization substantia nigra development cell junction assembly Fc-gamma receptor signaling pathway involved in phagocytosis ATP-dependent chromatin remodeling positive regulation of gene expression, epigenetic vascular endothelial growth factor receptor signaling pathway ephrin receptor signaling pathway platelet aggregation
          It has to be done as per old AB suggested Products section.
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