Invitrogen

beta Actin Polyclonal Antibody

8 Published Figures

21 References

View all (92) beta Actin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (7)
Published Figures (8)

beta Actin Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 15

beta Actin Antibody (PA1-46296) in ICC/IF

Immunofluorescence analysis of beta Actin was performed using 70% confluent log phase NIH/3T3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with beta Actin Rabbit Polyclonal Antibody (Product # PA1-46296) at 2µg/mL in 0.1% BSA and incubated for 3 hours a... View More

beta Actin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

beta Actin Antibody in Western Blot (WB)

beta Actin Antibody in Flow Cytometry (Flow)

beta Actin Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

beta Actin Antibody in Neutralization (Neu)

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Product Details

PA1-46296

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:2,000

View 19 publications 19 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2 µg/mL

Flow Cytometry (Flow)

1:10-1:1,000

ELISA (ELISA)

Assay-Dependent

Immunoprecipitation (IP)

1:10-1:500

Neutralization (Neu)

Assay-Dependent

View 1 publication 1 publication

in situ PLA (PLA)

Assay-Dependent

Product Specifications

Species Reactivity

Avian, Bovine, Fish, Hamster, Human, Mouse, Non-human primate, Pig, Rabbit, Rat

Published species

Bovine, Human, Mouse, Pig, Rat, Zebrafish

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from the N-terminus of Human beta-Actin.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

tris citrate/tris phosphate, pH 7-8

Contains

0.09% sodium azide

Storage conditions

4°C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2223196

Product Specific Information

This antibody is expected to cross-react with a wide range of species based on sequence homology.

Target Information

Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Elderberry extract improves molecular markers of endothelial dysfunction linked to atherosclerosis.

Food science & nutrition

Festa J,Hussain A,Hackney A,Desai U,Sahota TS,Singh H,Da Boit M

Published figure using beta Actin polyclonal antibody (Product # PA1-46296) in Western Blot

Sat Jul 01 00:00:00 UTC 2023

Epicatechin Prevents Cryocapacitation of Bovine Spermatozoa through Antioxidant Activity and Stabilization of Transmembrane Ion Channels.

International journal of molecular sciences

Baňas Š,Benko F,Ďuračka M,Lukáč N,Tvrdá E

PA1-46296 was used in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Neutralization, Proximity Ligation Assay, Western Blot to assess the potential protective abilities of Epicatechin on cryopreserved bovine spermatozoa.

Sat Jan 28 00:00:00 UTC 2023

Epicatechin Prevents Cryocapacitation of Bovine Spermatozoa through Antioxidant Activity and Stabilization of Transmembrane Ion Channels.

International journal of molecular sciences

Baňas Š,Benko F,Ďuračka M,Lukáč N,Tvrdá E

Sat Jan 28 00:00:00 UTC 2023

Protective effects of α-terpineol and Bacillus coagulans on intestinal function in weaned piglets infected with a recombinant Escherichia coli expressing heat-stable enterotoxin STa.

Frontiers in veterinary science

Wu T,Zhang Q,Xu H,Li P,Zhao D,Wang L,Yi D,Hou Y

PA1-46296 was used in Western Blot to suggest that α-TPN and B. coagulans can be used as antibiotic alternatives against ETEC infection in weaned piglets.

Tue Feb 28 00:00:00 UTC 2023

Cell clusters containing intestinal stem cells line, the zebrafish intestine intervillus pocket.

iScience

Tavakoli S,Zhu S,Matsudaira P

PA1-46296 was used in Immunohistochemistry (PFA fixed) to suggest that Prmt1 is a zebrafish ISC marker and the ISCs reside within basal cell clusters that are functionally analogous to crypts.

Fri May 20 00:00:00 UTC 2022

Betulonic Acid, as One of the Active Components of the Celastrus orbiculatus Extract, Inhibits the Invasion and Metastasis of Gastric Cancer Cells by Mediating Cytoskeleton Rearrangement In Vitro.

Molecules (Basel, Switzerland)

Chu Z,Luo Y,Ni T,Zhu M,Feng X,Liu Y,Wang H

PA1-46296 was used in Western Blotting to support that betulonic acid inhibits the invasion and metastasis of gastric cancer cells by mediating cytoskeletal remodeling and regulating epithelial mesenchymal transformation.

Wed Feb 02 00:00:00 UTC 2022

Poria Acid, Triterpenoids Extracted from Poria cocos, Inhibits the Invasion and Metastasis of Gastric Cancer Cells.

Molecules (Basel, Switzerland)

Wang H,Luo Y,Chu Z,Ni T,Ou S,Dai X,Zhang X,Liu Y

PA1-46296 was used in Western Blotting to show that Triterpenoids from P. cocos have significant biological activity against gastric cancer, and the mechanism may be involved in the process of epithelial-mesenchymal transformation.

Mon Jun 06 00:00:00 UTC 2022

Exosomal RNF157 mRNA from prostate cancer cells contributes to M2 macrophage polarization through destabilizing HDAC1.

Frontiers in oncology

Guan H,Mao L,Wang J,Wang S,Yang S,Wu H,Sun W,Chen Z,Chen M

PA1-46296 was used in Western Blot to investigate the role of exosomal RNF157 mRNA in prostate cancer development.

Fri Oct 21 00:00:00 UTC 2022

The Effect of Heat Shock on Myogenic Differentiation of Human Skeletal-Muscle-Derived Mesenchymal Stem/Stromal Cells.

Cells

Mikšiūnas R,Labeit S,Bironaitė D

PA1-46296 was used in Western Blotting to investigate the myogenic differentiation of CD56 (neural cell adhesion molecule, NCAM)-positive and -negative SM-MSCs and their response to the non-cytotoxic heat stimulus.

Thu Oct 13 00:00:00 UTC 2022

Multifaceted Effects and Mechanisms of N-Acetylcysteine on Intestinal Injury in a Porcine Epidemic Diarrhea Virus-Infected Porcine Model.

Molecular nutrition & food research

Zhang Q,Li P,Li H,Yi D,Guo S,Wang L,Zhao D,Wang C,Wu T,Hou Y

PA1-46296 was used in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Neutralization, Proximity Ligation Assay, Western Blot to suggest NAC exhibits multifaceted effects upon PEDV infection, and thus caution is required when using NAC as a dietary supplement to prevent viral infection.

Thu Dec 01 00:00:00 UTC 2022

Molecular neurobiological markers in the onset of sodium appetite.

Scientific reports

Porcari CY,Cambiasso MJ,Mecawi AS,Caeiro XE,Antunes-Rodrigues J,Vivas LM,Godino A

PA1-46296 was used in Western Blotting to indicate that SD exerts a coordinated timing effect, promoting the appearance of sodium appetite through changes in MAPK activity and lower Trpv1 channel and Oxtr expression that trigger sodium consumption to reestablish the hydroelectrolytic homeostasis.

Sat Aug 20 00:00:00 UTC 2022

CoQ10 augments candesartan protective effect against tourniquet-induced hind limb ischemia-reperfusion: Involvement of non-classical RAS and ROS pathways.

Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society

Awad AS,Nour El-Din M,Kamel R

PA1-46296 was used in Western Blotting to show the impact of CoQ10 on skeletal muscle renin-angiotensin system in rats.

Thu Jul 01 00:00:00 UTC 2021

CoQ10 augments candesartan protective effect against tourniquet-induced hind limb ischemia-reperfusion: Involvement of non-classical RAS and ROS pathways.

Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society

Awad AS,Nour El-Din M,Kamel R

PA1-46296 was used in Western Blotting to show the impact of CoQ10 on skeletal muscle renin-angiotensin system in rats.

Thu Jul 01 00:00:00 UTC 2021

Reduction of Renal Preservation/Reperfusion Injury by Controlled Hyperthermia During Ex Vivo Machine Perfusion.

Clinical and translational science

Minor T,von Horn C

PA1-46296 was used in Western Blotting to show that ex vivo heat-shock treatment seems to be an easily implementable and promising option to enhance renal self-defense machinery against reperfusion injury after preservation that merits further investigation in preclinical models.

Mon Mar 01 00:00:00 UTC 2021

Immune Synapse Residency of Orai1 Alters Ca²⁺ Response of T Cells.

International journal of molecular sciences

Voros O,Panyi G,Hajdu P

PA1-46296 was used in Western Blot to understand the background of Orai1 IS-redistribution we knocked down STIM1 and SAP97 (adaptor protein with a short IS-residency (15 min) and ability to bind Orai1 N-terminus): the mGFP-Orai1-Full channels kept on accumulating in the IS up to the 60th minute in the STIM1- and SAP97-lacking Jurkat cells.

Tue Oct 26 00:00:00 UTC 2021

Monolaurin Confers a Protective Effect Against Porcine Epidemic Diarrhea Virus Infection in Piglets by Regulating the Interferon Pathway.

Frontiers in immunology

Zhang Q,Yi D,Ji C,Wu T,Wang M,Guo S,Wang L,Zhao D,Hou Y

PA1-46296 was used in Western Blotting to study the effect of monolaurin (ML) on the growth performance, intestinal function, virus replication and cytokine response in piglets infected with porcine epidemic diarrhea virus (PEDV), and to reveal the mechanism through proteomics analysis.

Mon Feb 28 00:00:00 UTC 2022

Proteome Analysis of Human Natural Killer Cell Derived Extracellular Vesicles for Identification of Anticancer Effectors.

Molecules (Basel, Switzerland)

Choi JW,Lim S,Kang JH,Hwang SH,Hwang KC,Kim SW,Lee S

PA1-46296 was used in Neutralization experiments to evaluate the anticancer potential of isolated extracellular vesicles from expanded and activated NK-cell-enriched lymphocytes.

Mon Nov 09 00:00:00 UTC 2020

Proteome Analysis of Human Natural Killer Cell Derived Extracellular Vesicles for Identification of Anticancer Effectors.

Molecules (Basel, Switzerland)

Choi JW,Lim S,Kang JH,Hwang SH,Hwang KC,Kim SW,Lee S

PA1-46296 was used in Neutralization experiments to evaluate the anticancer potential of isolated extracellular vesicles from expanded and activated NK-cell-enriched lymphocytes.

Mon Nov 09 00:00:00 UTC 2020

Neuroprotective role of delta opioid receptors in hypoxic preconditioning.

Turkish journal of medical sciences

Güney Ş,Dinçer S,Göktaş G,Take-Kaplanoğlu G

PA1-46296 was used in Western Blotting to explore the neuroprotective role of delta opioid receptors (DOR) in the rat cortex in hypoxic preconditioning.

Thu Oct 24 00:00:00 UTC 2019

M1-Polarized Macrophages Promote Self-Renewing Phenotype of Hepatic Progenitor Cells with Jagged1-Notch Signalling Involved: Relevance in Primary Sclerosing Cholangitis.

Journal of immunology research

Li H,Sun S,Lei Q,Lei P,Cai X,Wan C,Shen G

PA1-46296 was used in Western Blot to suggest that M1 macrophages promote an HPC self-renewing phenotype which is associated with Notch signalling activation within HPC.

Mon Apr 08 00:00:00 UTC 2019

GFAP expression is influenced by astrocytoma grade and rs2070935 polymorphism.

Journal of Cancer

Sereika M,Urbanaviciute R,Tamasauskas A,Skiriute D,Vaitkiene P

Published figure using beta Actin polyclonal antibody (Product # PA1-46296) in Western Blot

Thu Oct 01 00:00:00 UTC 2020

Knockdown of COBRA1 decreases the proliferation and migration of hepatocellular carcinoma cells.

Oncology reports

El Zeneini E,Kamel S,El-Meteini M,Amleh A

PA1-46296 was used in Western Blotting to study the importance of COBRA1 in hepatocellular carcinoma cells proliferation and migration.

Wed Mar 01 00:00:00 UTC 2017

Gestation age dependent transfer of human immunoglobulins across placenta in timed-pregnant guinea pigs.

Placenta

Xu Y,Ma L,Norton MG,Stuart C,Zhao Z,Toibero D,Dahlen S,Zhong L,Zhang P,Struble EB

PA1-46296 was used in western blot to study timed-pregnant guinea pigs and gestation age dependent transfer of human immunoglobulins across placenta

Tue Dec 01 00:00:00 UTC 2015

Molecular Magnetic Resonance Imaging of Tumor Response to Therapy.

Scientific reports

Shuhendler AJ,Ye D,Brewer KD,Bazalova-Carter M,Lee KH,Kempen P,Dane Wittrup K,Graves EE,Rutt B,Rao J

Published figure using beta Actin polyclonal antibody (Product # PA1-46296) in Western Blot

Tue Oct 06 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: actin, beta; Actin, cytoplasmic 1; beta cytoskeletal actin; Beta-actin; cytoplasmic beta-actin; gamma non-muscle actin; PS1TP5-binding protein 1

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Gene Aliases: ACT-5; ACTB; Actx; Beta-actin; BOS_22932; BRWS1; E430023M04Rik; PS1TP5BP1

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UniProt ID: (Rabbit) P29751, (Bovine) P60712, (Human) P60709, (Pig) Q6QAQ1, (Rat) P60711, (Mouse) P60710, (Chinese hamster) P48975

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Entrez Gene ID: (Rabbit) 100009272, (Bovine) 280979, (Human) 60, (Pig) 414396, (Rat) 81822, (Mouse) 11461, (Chinese hamster) 100689477

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Function(s)

ATP binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding structural constituent of cytoskeleton protein binding kinesin binding Tat protein binding nucleosomal DNA binding identical protein binding nitric-oxide synthase binding protein kinase binding

Process(es)

protein folding chaperone mediated protein folding independent of cofactor retina homeostasis movement of cell or subcellular component cytoskeleton organization substantia nigra development cell junction assembly Fc-gamma receptor signaling pathway involved in phagocytosis ATP-dependent chromatin remodeling positive regulation of gene expression, epigenetic vascular endothelial growth factor receptor signaling pathway ephrin receptor signaling pathway platelet aggregation axonogenesis retina development in camera-type eye cellular response to electrical stimulus

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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beta Actin Polyclonal Antibody

beta Actin Antibody (PA1-46296) in ICC/IF

Product Details

PA1-46296

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Neutralization (Neu)

in situ PLA (PLA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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