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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • beta Actin Antibodies

          Invitrogen

          beta Actin Loading Control Monoclonal Antibody (BA3R), DyLight™ 488

          Advanced Verification
          6 Published Figures
          83 References
          View all (92) beta Actin antibodies

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          Datasheet
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          Datasheet
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          Cite beta Actin Loading Control Monoclonal Antibody (BA3R), DyLight™ 488

          • Antibody Testing Data (2)
          • Published Figures (6)
          • Advanced Verification (1)
          beta Actin Loading Control Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          beta Actin Loading Control Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 9

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          beta Actin Loading Control Antibody (MA5-15739-D488) in ICC/IF

          Immunofluorescent analysis of beta-actin (green) in HeLa cells. Cells were fixed with either 4% paraformaldehyde (PFA) for 15 minutes at room temperature followed by permeabilization with 0.5% Triton X-100 in PBS for 15 minutes at room temperature (left panel) or fixed and permeabilized with ice-cold methanol for 10 minutes at room temperature (right panel). Cells were blocked with 0.3% BSA in PBS for 15 mi... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          beta Actin Loading Control Antibody in Immunocytochemistry (ICC/IF)
          beta Actin Loading Control Antibody in Western Blot (WB)
          beta Actin Loading Control Antibody in Western Blot (WB)
          beta Actin Loading Control Antibody in Western Blot (WB)
          beta Actin Loading Control Antibody in Western Blot (WB)
          beta Actin Loading Control Antibody in Western Blot (WB)
          beta Actin Loading Control Antibody in Western Blot (WB)
          beta Actin Loading Control Antibody in Western Blot (WB)
          beta Actin Loading Control Antibody

          Product Details

          MA5-15739-D488

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          View 83 publications 83 publications

          Immunocytochemistry (ICC/IF)

          1:20 - 1:100
          -
          Product Specifications

          Species Reactivity

          Chicken, Human, Mouse, Rabbit, Rat

          Published species

          Guinea pig, Human, Mouse, Plant, Rabbit, Rat

          Host/Isotype

          Mouse / IgG2b

          Class

          Monoclonal

          Type

          Antibody

          Clone

          BA3R

          Immunogen

          Beta-actin N-terminal peptide.
          View immunogen

          Conjugate

          DyLight™ 488 DyLight™ 488 DyLight™ 488
          • Unconjugated
          • Biotin 
          • DyLight 550
          • DyLight 650
          • DyLight 680
          • DyLight 800 4X PEG
          • HRP
          • Request custom conjugation

          Excitation/Emission Max

          492/519 nm View spectra spectra

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Protein A

          Storage buffer

          sodium bicarbonate

          Contains

          no preservative

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2537662

          Product Specific Information

          MA5-15739-D488 detects beta actin from human, mouse, rat, rabbit, and chicken samples.

          MA5-15739-D488 has been successfully used in immunofluorescence. DyLight 488 has an excitation/emission of 493/518 nm.

          For visualization of actin filaments by immunofluorescence, fixation and permeabilization with methanol is required.

          Target Information

          Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: actin, beta; Actin, cytoplasmic 1; beta cytoskeletal actin; Beta-actin; cytoplasmic 1; cytoplasmic beta-actin; gamma non-muscle actin; PS1TP5-binding protein 1

          View more View less

          Gene Aliases: ACT-5; ACTB; actin; Actx; Bact; beta-actin; BRWS1; E430023M04Rik; PS1TP5BP1; RCJMB04_4h19

          View more View less

          UniProt ID: (Rabbit) P29751, (Human) P60709, (Chicken) P60706, (Rat) P60711, (Mouse) P60710

          View more View less

          Entrez Gene ID: (Rabbit) 100009272, (Human) 60, (Chicken) 396526, (Rat) 81822, (Mouse) 11461

          View more View less

          Function(s)
          RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding structural constituent of cytoskeleton protein binding ATP binding kinesin binding Tat protein binding nucleosomal DNA binding identical protein binding nitric-oxide synthase binding protein kinase binding
          Process(es)
          retina homeostasis movement of cell or subcellular component cytoskeleton organization substantia nigra development cell junction assembly Fc-gamma receptor signaling pathway involved in phagocytosis ATP-dependent chromatin remodeling positive regulation of gene expression, epigenetic vascular endothelial growth factor receptor signaling pathway ephrin receptor signaling pathway platelet aggregation axonogenesis retina development in camera-type eye cellular response to electrical stimulus
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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