Invitrogen

beta Amyloid (1-40) Polyclonal Antibody

9 Published Figures

23 References

View all (4) Amyloid beta 40 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (9)

FIGURE: 1 / 10

beta Amyloid (1-40) Antibody (44-136)

Amyloid Beta (Abeta) (N-terminal) Polyclonal Antibody, Rabbit

beta Amyloid (1-40) Antibody in Immunohistochemistry (IHC)

beta Amyloid (1-40) Antibody in Western Blot (WB)

beta Amyloid (1-40) Antibody in ELISA (ELISA)

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Product Details

44-136

Applications

Tested Dilution

Publications

Western Blot (WB)

View 5 publications 5 publications

Immunohistochemistry (IHC)

Assay-dependent

View 10 publications 10 publications

Immunohistochemistry (Frozen) (IHC (F))

View 3 publications 3 publications

Immunohistochemistry - Free Floating (IHC (Free))

View 1 publication 1 publication

ELISA (ELISA)

1 µg/mL

View 3 publications 3 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

Radioimmune Assays (RIA)

Assay-dependent

Dot blot (DB)

Assay-dependent

Product Specifications

Species Reactivity

Human

Published species

Bacteria, Cat, Human, Mouse, Non-human primate

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The antiserum was produced against a chemically synthesized peptide corresponding to amino acids 1-40 of human beta-Amyloid (A-beta).

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533599

Product Specific Information

No significant cross-reactivity to beta-Amyloid 42 has been observed. Previous lots of this antibody recognized the sequence of beta-Amyloid [1-40] in the region from amino acids 15-30. No blocking activity was observed with beta-Amyloid [1-12] whereas the peptides beta-Amyloid [14-35], beta-Amyloid [15-28], beta-Amyloid [17-30] and Beta-Amyloid [1-40] were all able to block antibody activity.

Target Information

Beta-amyloid is a 40-43 amino acid peptide cleaved from amyloid precursor protein, and a putative gamma-secretase. Amyloid beta (1-40) together with Amyloid beta (1-42) are two major C-terminal variants of the amyloid beta protein, constituting the majority of amyloid beta proteins. Both the amyloid beta (40) and amyloid beta (42) alloforms are major components of the extracellular plaques found in Alzheimer's disease brain tissue.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Blood-brain barrier penetrating neprilysin degrades monomeric amyloid-beta in a mouse model of Alzheimer's disease.

Alzheimer's research & therapy

Rofo F,Metzendorf NG,Saubi C,Suominen L,Godec A,Sehlin D,Syvänen S,Hultqvist G

44-136 was used in Enzyme-linked immunosorbent assay to show that a one-time injection of a BBB-penetrating NEP shows the potential to reduce, the likely most toxic, Aβ oligomers in the brain in addition to monomers. Also, Aβ aggregates in the blood were reduced.

Mon Dec 05 00:00:00 UTC 2022

The Impact of the hAPP695SW Transgene and Associated Amyloid-β Accumulation on Murine Hippocampal Biochemical Pathways.

Journal of Alzheimer's disease : JAD

Khorani M,Bobe G,Matthews DG,Magana AA,Caruso M,Gray NE,Quinn JF,Stevens JF,Soumyanath A,Maier CS

44-136 was used in Immunohistochemistry-immunofluorescence to suggest that dysregulation of multiple metabolic pathways associated with a concomitant shift to an excitatory-inhibitory imbalance are contributing mechanisms of Alzheimer's disease-related pathology in the Tg2576 mouse.

Thu Mar 03 00:00:00 UTC 2022

Enhanced neprilysin-mediated degradation of hippocampal Aβ42 with a somatostatin peptide that enters the brain.

Theranostics

Rofo F,Ugur Yilmaz C,Metzendorf N,Gustavsson T,Beretta C,Erlandsson A,Sehlin D,Syvänen S,Nilsson P,Hultqvist G

Published figure using beta Amyloid (1-40) polyclonal antibody (Product # 44-136) in ELISA

Wed Aug 04 00:00:00 UTC 2021

Low-Dose Phosphodiesterase III Inhibitor Reduces the Vascular Amyloid Burden in Amyloid-β Protein Precursor Transgenic Mice.

International journal of molecular sciences

Yakushiji Y,Kawamoto K,Uchihashi K,Ihara M,Aoki S,Nagaishi Y,Suzuyama K,Tsugitomi Y,Hara H

44-136 was used in Immunohistochemistry to investigate whether lower-dose cilostazol can decrease micro-hemorrhages and amyloid-β (Aβ) deposition in the brain using Aβ Precursor Protein (APP) transgenic mice.

Thu Mar 26 00:00:00 UTC 2020

Low-Dose Phosphodiesterase III Inhibitor Reduces the Vascular Amyloid Burden in Amyloid-β Protein Precursor Transgenic Mice.

International journal of molecular sciences

Yakushiji Y,Kawamoto K,Uchihashi K,Ihara M,Aoki S,Nagaishi Y,Suzuyama K,Tsugitomi Y,Hara H

Thu Mar 26 00:00:00 UTC 2020

Centella Asiatica Improves Memory and Promotes Antioxidative Signaling in 5XFAD Mice.

Antioxidants (Basel, Switzerland)

Matthews DG,Caruso M,Murchison CF,Zhu JY,Wright KM,Harris CJ,Gray NE,Quinn JF,Soumyanath A

44-136 was used in Immunohistochemistry to study the effect of Centella asiatica water extract treatment on oxidative stress in Alzheimer's disease model mice.

Sun Dec 08 00:00:00 UTC 2019

A 4R tauopathy develops without amyloid deposits in aged cat brains.

Neurobiology of aging

Poncelet L,Ando K,Vergara C,Mansour S,Suain V,Yilmaz Z,Reygel A,Gilissen E,Brion JP,Leroy K

44-136 was used in Immunohistochemistry-immunofluorescence to demonstrate that the presence of a tau pathology in aged cat brains that develop independently of amyloid deposits.

Sun Sep 01 00:00:00 UTC 2019

Effects of rising amyloidβ levels on hippocampal synaptic transmission, microglial response and cognition in APP_Swe/PSEN1_M146V transgenic mice.

EBioMedicine

Medawar E,Benway TA,Liu W,Hanan TA,Haslehurst P,James OT,Yap K,Muessig L,Moroni F,Nahaboo Solim MA,Baidildinova G,Wang R,Richardson JC,Cacucci F,Salih DA,Cummings DM,Edwards FA

44-136 was used in Immunohistochemistry-immunofluorescence to investigate CA1 synaptic transmission and plasticity.

Tue Jan 01 00:00:00 UTC 2019

Corticotropin-releasing factor overexpression gives rise to sex differences in Alzheimer's disease-related signaling.

Molecular psychiatry

Bangasser DA,Dong H,Carroll J,Plona Z,Ding H,Rodriguez L,McKennan C,Csernansky JG,Seeholzer SH,Valentino RJ

44136 was used in immunohistochemistry to discuss the role of corticotropin-releasing factor in the sex bias of some neuropsychiatric and neurodegenerative disorders

Tue Aug 01 00:00:00 UTC 2017

Evidence of Tau Hyperphosphorylation and Dystrophic Microglia in the Common Marmoset.

Frontiers in aging neuroscience

Rodriguez-Callejas JD,Fuchs E,Perez-Cruz C

44136 was used in immunohistochemistry - frozen section to evaluate cellular markers associated with neurodegenerative diseases in common marmoset (Callithrix jacchus) brains

Thu Oct 01 00:00:00 UTC 2020

Amyloid-β oligomerization in Alzheimer dementia versus high-pathology controls.

Annals of neurology

Esparza TJ,Zhao H,Cirrito JR,Cairns NJ,Bateman RJ,Holtzman DM,Brody DL

Published figure using beta Amyloid (1-40) polyclonal antibody (Product # 44-136) in Western Blot

Tue Jan 01 00:00:00 UTC 2013

Amyloid-β oligomerization in Alzheimer dementia versus high-pathology controls.

Annals of neurology

Esparza TJ,Zhao H,Cirrito JR,Cairns NJ,Bateman RJ,Holtzman DM,Brody DL

Published figure using beta Amyloid (1-40) polyclonal antibody (Product # 44-136) in Western Blot

Tue Jan 01 00:00:00 UTC 2013

Suppressed accumulation of cerebral amyloid {beta} peptides in aged transgenic Alzheimer's disease mice by transplantation with wild-type or prostaglandin E2 receptor subtype 2-null bone marrow.

The American journal of pathology

Keene CD,Chang RC,Lopez-Yglesias AH,Shalloway BR,Sokal I,Li X,Reed PJ,Keene LM,Montine KS,Breyer RM,Rockhill JK,Montine TJ

Published figure using beta Amyloid (1-40) polyclonal antibody (Product # 44-136) in Immunofluorescence

Thu Jul 01 00:00:00 UTC 2010

SRF and myocardin regulate LRP-mediated amyloid-beta clearance in brain vascular cells.

Nature cell biology

Bell RD,Deane R,Chow N,Long X,Sagare A,Singh I,Streb JW,Guo H,Rubio A,Van Nostrand W,Miano JM,Zlokovic BV

44-136 was used in Immunofluorescence in immunohistochemistry on frozen tissues to suggest that serum response factor and myocardin function as a transcriptional switch, controlling Abeta cerebrovascular clearance and Alzheimer's disease progression.

Sun Feb 01 00:00:00 UTC 2009

SRF and myocardin regulate LRP-mediated amyloid-beta clearance in brain vascular cells.

Nature cell biology

Bell RD,Deane R,Chow N,Long X,Sagare A,Singh I,Streb JW,Guo H,Rubio A,Van Nostrand W,Miano JM,Zlokovic BV

Sun Feb 01 00:00:00 UTC 2009

Selective contrast enhancement of individual Alzheimer's disease amyloid plaques using a polyamine and Gd-DOTA conjugated antibody fragment against fibrillar Abeta42 for magnetic resonance molecular imaging.

Pharmaceutical research

Ramakrishnan M,Wengenack TM,Kandimalla KK,Curran GL,Gilles EJ,Ramirez-Alvarado M,Lin J,Garwood M,Jack CR,Poduslo JF

44-136 was used in immunohistochemistry - frozen section to describe using the F(ab')2 fragment of a monoclonal antibody raised against fibrillar human Abeta42 as a diagnostic imaging probe for AD

Fri Aug 01 00:00:00 UTC 2008

Histamine induces Egr-1 expression in human aortic endothelial cells via the H1 receptor-mediated protein kinase Cdelta-dependent ERK activation pathway.

The Journal of biological chemistry

Hao F,Tan M,Xu X,Cui MZ

Fri Oct 03 00:00:00 UTC 2008

Intraneuronal beta-amyloid aggregates, neurodegeneration, and neuron loss in transgenic mice with five familial Alzheimer's disease mutations: potential factors in amyloid plaque formation.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Oakley H,Cole SL,Logan S,Maus E,Shao P,Craft J,Guillozet-Bongaarts A,Ohno M,Disterhoft J,Van Eldik L,Berry R,Vassar R

Wed Oct 04 00:00:00 UTC 2006

Time-dependent reduction in Abeta levels after intracranial LPS administration in APP transgenic mice.

Experimental neurology

Herber DL,Roth LM,Wilson D,Wilson N,Mason JE,Morgan D,Gordon MN

44-136 was used in immunohistochemistry - free floating to report that activated brain glia rapidly and transiently clear diffuse Abeta deposits but not compacted fibrillar amyloid

Mon Nov 01 00:00:00 UTC 2004

Efficient inhibition of beta-secretase gene expression in HEK293 cells by tRNAVal-driven and CTE-helicase associated hammerhead ribozymes.

European journal of biochemistry

Nawrot B,Antoszczyk S,Maszewska M,Kuwabara T,Warashina M,Taira K,Stec WJ

Wed Oct 01 00:00:00 UTC 2003

Efficient inhibition of beta-secretase gene expression in HEK293 cells by tRNAVal-driven and CTE-helicase associated hammerhead ribozymes.

European journal of biochemistry

Nawrot B,Antoszczyk S,Maszewska M,Kuwabara T,Warashina M,Taira K,Stec WJ

Wed Oct 01 00:00:00 UTC 2003

Human and murine ApoE markedly alters A beta metabolism before and after plaque formation in a mouse model of Alzheimer's disease.

Neurobiology of disease

Fagan AM,Watson M,Parsadanian M,Bales KR,Paul SM,Holtzman DM

Mon Apr 01 00:00:00 UTC 2002

Apolipoprotein E4 influences amyloid deposition but not cell loss after traumatic brain injury in a mouse model of Alzheimer's disease.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Hartman RE,Laurer H,Longhi L,Bales KR,Paul SM,McIntosh TK,Holtzman DM

Sun Dec 01 00:00:00 UTC 2002

Plaque-associated disruption of CSF and plasma amyloid-beta (Abeta) equilibrium in a mouse model of Alzheimer's disease.

Journal of neurochemistry

DeMattos RB,Bales KR,Parsadanian M,O'Dell MA,Foss EM,Paul SM,Holtzman DM

Mon Apr 01 00:00:00 UTC 2002

Plaque-associated disruption of CSF and plasma amyloid-beta (Abeta) equilibrium in a mouse model of Alzheimer's disease.

Journal of neurochemistry

DeMattos RB,Bales KR,Parsadanian M,O'Dell MA,Foss EM,Paul SM,Holtzman DM

Mon Apr 01 00:00:00 UTC 2002

Passive immunization against beta-amyloid peptide protects central nervous system (CNS) neurons from increased vulnerability associated with an Alzheimer's disease-causing mutation.

The Journal of biological chemistry

Mohajeri MH,Saini K,Schultz JG,Wollmer MA,Hock C,Nitsch RM

Fri Sep 06 00:00:00 UTC 2002

Bioinformatics

Protein Aliases: A beta 1 40; A ß 1 40; AB40; ABPP; Alzheimer disease amyloid A4 protein homolog; Alzheimer disease amyloid protein; Amyloid; Amyloid b; Amyloid beta; amyloid beta (A4) precursor protein; Amyloid beta 1 40; Amyloid precursor protein; Amyloid β; Amyloid-beta (A4) precursor protein; Amyloid-beta A4 protein; Amyloid-beta precursor protein; APP; APPI; Aß 1 40; B APP40; BAPP40; beta-amyloid peptide; beta-amyloid peptide(1-40); beta-amyloid peptide(1-42); beta-amyloid precursor protein; BetaAPP40; Cerebral vascular amyloid peptide; CVAP; peptidase nexin-II; PN-II; PreA4; Protease nexin-II; testicular tissue protein Li 2; ß APP40; ßAPP40

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Gene Aliases: A4; AAA; ABETA; ABPP; AD1; APP; APPI; CTFgamma; CVAP; PN-II; PN2

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UniProt ID: (Human) P05067

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Entrez Gene ID: (Human) 351

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Function(s)

DNA binding serine-type endopeptidase inhibitor activity receptor binding protein binding heparin binding peptidase activator activity enzyme binding identical protein binding transition metal ion binding PTB domain binding growth factor receptor binding

Process(es)

response to yeast suckling behavior platelet degranulation mRNA polyadenylation regulation of translation protein phosphorylation cellular copper ion homeostasis endocytosis response to oxidative stress cell adhesion regulation of epidermal growth factor-activated receptor activity Notch signaling pathway axonogenesis mating behavior locomotory behavior axo-dendritic transport cholesterol metabolic process adult locomotory behavior visual learning cellular process response to lead ion negative regulation of endopeptidase activity positive regulation of peptidase activity positive regulation of G2/M transition of mitotic cell cycle axon midline choice point recognition neuron remodeling dendrite development antibacterial humoral response antifungal humoral response extracellular matrix organization forebrain development neuron projection development ionotropic glutamate receptor signaling pathway regulation of multicellular organism growth regulation of protein binding cellular protein metabolic process innate immune response negative regulation of neuron differentiation positive regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter collateral sprouting in absence of injury regulation of synapse structure or activity defense response to Gram-negative bacterium defense response to Gram-positive bacterium neuromuscular process controlling balance synaptic growth at neuromuscular junction neuron apoptotic process smooth endoplasmic reticulum calcium ion homeostasis cellular response to cAMP cellular response to norepinephrine stimulus amyloid fibril formation cellular response to nerve growth factor stimulus

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beta Amyloid (1-40) Polyclonal Antibody

beta Amyloid (1-40) Antibody (44-136)

Product Details

44-136

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunohistochemistry - Free Floating (IHC (Free))

ELISA (ELISA)

Immunoprecipitation (IP)

Radioimmune Assays (RIA)

Dot blot (DB)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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