Invitrogen

beta-Arrestin 1,2 Polyclonal Antibody

2 Published Figures
8 References

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA1-730

Applications
Tested Dilution
Publications

Western Blot (WB)

3 µg/mL
View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-

Immunocytochemistry (ICC/IF)

-
View 3 publications 3 publications

Immunoprecipitation (IP)

Assay-dependent
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human, Rat

Published species

Fruit fly, Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptide: C D(384) D I V F E D F A R L R L K(397)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2274371

Product Specific Information

PA1-730 detects recombinant rat and human beta-arrestin and beta-arrestin2. This antibody does not detect visual or cone arrestin.

PA1-730 has been successfully used in Western blot, Immunohistochemistry (paraffin) and immunoprecipitation procedures. By Western blot, this antibody detects ~49 kDa and ~47 kDa proteins representing recombinant beta-arrestin and beta-arrestin2, respectively.

The PA1-730 immunizing peptide corresponds to amino acid residues 384-397 from human beta-arrestin2. This peptide (Cat. # PEP-156) is available for use in neutralization and control experiments.

Target Information

Beta Arrestin 1 is a member of a family of proteins widely expressed but especially abundant in the central nervous system. Serving as an adaptor or scaffold maolecule, beta Arrestin 1 is essential for mitogenic signalling and mediates agonist-dependent desensitization and internalization of Gprotein-coupled receptors (GPCRs, e.g., beta 2-adrenergic receptor). After binding to their ligand and interacting with heterotrimeric G proteins, GPCRs are phosporylated by G-protein receptor kinases (GRKs) on serine residues. Beta Arrestin 1 in the cytosol is phosphorylated by ERK1&2 on serine412 in a negative feedback mechanism and binds to the phosphorylated receptors at the plasma membrane. Serine 412 is then dephosphorylated and the GPCRs are internalized, leading to activation of the Ras, Raf, ERK1&2 signaling pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: arrestin 2; arrestin 3; Arrestin beta-1; Arrestin beta-2; arrestin, beta 1; arrestin, beta 2; Beta-arrestin-1; Beta-arrestin-2; Non-visual arrestin-2; Non-visual arrestin-3

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Gene Aliases: ARB1; ARB2; ARR1; ARR2; ARRB1; ARRB2; BARR2; BARRES

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UniProt ID: (Human) P49407, (Human) P32121, (Rat) P29066, (Rat) P29067

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Entrez Gene ID: (Human) 408, (Human) 409, (Rat) 25387, (Rat) 25388

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Function(s)
histone acetyltransferase activity enzyme inhibitor activity GTPase activator activity insulin-like growth factor receptor binding protein binding transcription factor binding estrogen receptor binding mitogen-activated protein kinase kinase binding ubiquitin protein ligase binding alpha-1A adrenergic receptor binding alpha-1B adrenergic receptor binding angiotensin receptor binding follicle-stimulating hormone receptor binding V2 vasopressin receptor binding AP-2 adaptor complex binding clathrin adaptor activity cysteine-type endopeptidase inhibitor activity involved in apoptotic process transcription regulatory region DNA binding ion channel binding protein phosphorylated amino acid binding G-protein coupled receptor binding receptor binding enzyme binding protein domain specific binding type 1 angiotensin receptor binding D1 dopamine receptor binding type 2A serotonin receptor binding platelet activating factor receptor binding protein complex binding protein complex scaffold protein kinase B binding mitogen-activated protein kinase binding 14-3-3 protein binding clathrin binding phosphoprotein binding
Process(es)
activation of MAPK activity positive regulation of protein phosphorylation G-protein coupled receptor internalization positive regulation of receptor internalization transcription from RNA polymerase II promoter apoptotic process phototransduction protein transport protein ubiquitination platelet activation negative regulation of protein ubiquitination positive regulation of protein ubiquitination negative regulation of NF-kappaB transcription factor activity positive regulation of protein binding negative regulation of interleukin-6 production negative regulation of interleukin-8 production positive regulation of peptidyl-serine phosphorylation negative regulation of GTPase activity positive regulation of smooth muscle cell apoptotic process positive regulation of Rho protein signal transduction positive regulation of histone acetylation positive regulation of insulin secretion involved in cellular response to glucose stimulus response to antidepressant follicle-stimulating hormone signaling pathway stress fiber assembly negative regulation of cysteine-type endopeptidase activity involved in apoptotic process proteasome-mediated ubiquitin-dependent protein catabolic process positive regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of GTPase activity positive regulation of transcription from RNA polymerase II promoter negative regulation of ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade positive regulation of histone H4 acetylation desensitization of G-protein coupled receptor protein signaling pathway by arrestin transforming growth factor beta receptor signaling pathway dopamine receptor signaling pathway brain development adult walking behavior receptor internalization positive regulation of synaptic transmission, dopaminergic negative regulation of interleukin-1 beta production negative regulation of interleukin-12 production negative regulation of tumor necrosis factor production negative regulation of toll-like receptor signaling pathway negative regulation of smooth muscle cell apoptotic process negative regulation of natural killer cell mediated cytotoxicity positive regulation of peptidyl-tyrosine phosphorylation detection of temperature stimulus involved in sensory perception of pain positive regulation of protein kinase B signaling negative regulation of protein kinase B signaling positive regulation of calcium ion transport Wnt signaling pathway, planar cell polarity pathway cell chemotaxis regulation of androgen receptor signaling pathway negative regulation of release of cytochrome c from mitochondria chemical synaptic transmission, postsynaptic positive regulation of DNA biosynthetic process negative regulation of protein phosphorylation endocytosis receptor-mediated endocytosis G-protein coupled receptor signaling pathway adenylate cyclase-modulating G-protein coupled receptor signaling pathway regulation of G-protein coupled receptor protein signaling pathway sensory perception of pain regulation of cAMP biosynthetic process regulation of cAMP catabolic process sensory perception of touch regulation of protein phosphorylation
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