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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • beta COP Antibodies

          Invitrogen

          beta COP Polyclonal Antibody

          Advanced Verification
          15 Published Figures
          57 References
          View all (10) beta COP antibodies

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          Datasheet
          Protocols
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          Datasheet
          Protocols
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          Cite beta COP Polyclonal Antibody

          • Antibody Testing Data (4)
          • Published Figures (15)
          • Advanced Verification (1)
          beta COP Antibody in Immunocytochemistry (ICC/IF)
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          beta COP Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 20

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          beta COP Antibody (PA1-061) in ICC/IF

          Immunofluorescent analysis of beta COP was performed using 70% confluent log phase L6 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with beta COP Rabbit Polyclonal Antibody (Product # PA1-061) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product #... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          beta COP Antibody in Immunocytochemistry (ICC/IF)
          beta COP Antibody in Immunocytochemistry (ICC/IF)
          beta COP Antibody in Western Blot (WB)
          beta COP Antibody in Western Blot (WB)
          beta COP Antibody in Immunocytochemistry (ICC/IF)
          beta COP Antibody in Immunocytochemistry (ICC/IF)
          beta COP Antibody in Immunocytochemistry (ICC/IF)
          beta COP Antibody in Immunocytochemistry (ICC/IF)
          beta COP Antibody in Western Blot, Immunocytochemistry, Immunoprecipitation (WB, ICC/IF, IP)
          beta COP Antibody in Immunocytochemistry (ICC/IF)
          beta COP Antibody in Immunocytochemistry (ICC/IF)
          beta COP Antibody in Immunocytochemistry (ICC/IF)
          beta COP Antibody in Western Blot (WB)
          beta COP Antibody in Western Blot (WB)
          beta COP Antibody in Western Blot (WB)
          beta COP Antibody in Western Blot, Immunoprecipitation (WB, IP)
          beta COP Antibody in Western Blot (WB)
          beta COP Antibody in Western Blot, Immunoprecipitation (WB, IP)
          beta COP Antibody in Western Blot (WB)
          beta COP Antibody

          Product Details

          PA1-061

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          2 µg/mL
          View 11 publications 11 publications

          Immunohistochemistry (IHC)

          -
          View 2 publications 2 publications

          Immunocytochemistry (ICC/IF)

          2 µg/mL
          View 42 publications 42 publications

          Immunoprecipitation (IP)

          -
          View 2 publications 2 publications
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Bovine, Hamster, Human, Mouse, Non-human primate, Rat, Yeast

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Synthetic peptide corresponding to residues E(496) A G E L K P E E E I T V G P V Q K(513) of rat beta-COP.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          4.4 mg/mL

          Purification

          Ammonium sulfate precipitation

          Storage buffer

          PBS with 1mg/mL BSA

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2081296

          Product Specific Information

          PA1-061 detects Golgi beta-coatomer protein (beta-COP) from mouse, human and rat tissues.

          PA1-061 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~110kDa protein which represents beta-COP from rat brain homogenate. Indirect immunofluorescence staining of beta-COP in NIH-3T3 cells with PA1-061 results in beta-COP localization to the Golgi complex and to distinct vesicular structures scattered throughout the cytoplasm. Golgi localization of beta-COP is sensitive to treatment with brefeldin A or ATP depletion.

          The PA1-061 immunogen is a synthetic peptide corresponding to residues E(496) A G E L K P E E E I T V G P V Q K(513) of rat beta-COP. This sequence is completely conserved in human beta-COP.

          Target Information

          This gene encodes a protein subunit of the coatomer complex associated with non-clathrin coated vesicles. The coatomer complex, also known as the coat protein complex 1, forms in the cytoplasm and is recruited to the Golgi by activated guanosine triphosphatases. Once at the Golgi membrane, the coatomer complex may assist in the movement of protein and lipid components back to the endoplasmic reticulum. Alternatively spliced transcript variants have been described.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: beta coat protein; Beta-coat protein; Beta-COP; coatomer protein complex, subunit beta 1; Coatomer subunit beta; DKFZp761K102; FLJ10341; FLJ46444; FLJ57957; receptor for activated C-kinase-2

          View more View less

          Gene Aliases: 2610019B04Rik; COPB; COPB1; MSTP026; Rack2

          View more View less

          UniProt ID: (Human) P53618, (Mouse) Q9JIF7, (Rat) P23514

          View more View less

          Entrez Gene ID: (Human) 1315, (Mouse) 70349, (Rat) 114023

          View more View less

          Function(s)
          structural molecule activity protein binding
          Process(es)
          transport intracellular protein transport intra-Golgi vesicle-mediated transport protein transport vesicle-mediated transport ER to Golgi vesicle-mediated transport retrograde vesicle-mediated transport, Golgi to ER viral process
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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