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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • beta Galactosidase Antibodies

          Invitrogen

          beta Galactosidase Polyclonal Antibody

          45 Published Figures
          111 References
          View all (65) beta Galactosidase antibodies

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          Datasheet
          Protocols
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          Datasheet
          Protocols
          Questions & Answers

          Cite beta Galactosidase Polyclonal Antibody

          • Published Figures (45)
          beta Galactosidase Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          beta Galactosidase Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 45

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          beta Galactosidase Antibody (A-11132) in ICC/IF

          Cells 2021 - Figure 4 Confocal microscopy images show an increased expression of beta-galactosidase (green fluorescence) as the relative fluorescence units/area at 4 weeks but not at 12 weeks ( Table 2 ). Podocalyxin (red fluorescence) identifies the podocytes within the glomerulus. Unilateral nephrectomy is associated with glomerular hypertrophy. The bar graph shows the net relative fluorescence units/glomerulus. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          beta Galactosidase Antibody in Immunocytochemistry (ICC/IF)
          beta Galactosidase Antibody in Immunocytochemistry (ICC/IF)
          beta Galactosidase Antibody in Immunocytochemistry (ICC/IF)
          beta Galactosidase Antibody in Immunocytochemistry (ICC/IF)
          beta Galactosidase Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          beta Galactosidase Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          beta Galactosidase Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          beta Galactosidase Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          beta Galactosidase Antibody in Immunocytochemistry (ICC/IF)
          beta Galactosidase Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))
          beta Galactosidase Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))
          beta Galactosidase Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))
          beta Galactosidase Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          beta Galactosidase Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          beta Galactosidase Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          beta Galactosidase Antibody in Immunohistochemistry (Frozen) (IHC (F))
          beta Galactosidase Antibody in Immunohistochemistry (Frozen) (IHC (F))
          beta Galactosidase Antibody in Immunohistochemistry (IHC)
          beta Galactosidase Antibody in Immunohistochemistry (IHC)
          beta Galactosidase Antibody in Immunohistochemistry (IHC)
          beta Galactosidase Antibody in Immunohistochemistry (IHC)
          beta Galactosidase Antibody in Immunohistochemistry (IHC)
          beta Galactosidase Antibody in Immunohistochemistry (IHC)
          beta Galactosidase Antibody in Immunohistochemistry (IHC)
          beta Galactosidase Antibody in Immunohistochemistry (IHC)
          beta Galactosidase Antibody in Immunohistochemistry (IHC)
          beta Galactosidase Antibody in Immunohistochemistry (IHC)
          beta Galactosidase Antibody in Immunohistochemistry (IHC)
          beta Galactosidase Antibody in Immunohistochemistry (IHC)
          beta Galactosidase Antibody in Immunohistochemistry (IHC)
          beta Galactosidase Antibody in Immunohistochemistry (IHC)
          beta Galactosidase Antibody in Immunohistochemistry (IHC)
          beta Galactosidase Antibody in Immunohistochemistry (IHC)
          beta Galactosidase Antibody in Immunohistochemistry (IHC)
          beta Galactosidase Antibody in Immunohistochemistry (IHC)
          beta Galactosidase Antibody in Immunohistochemistry (IHC)
          beta Galactosidase Antibody in Immunohistochemistry (IHC)
          beta Galactosidase Antibody in Immunohistochemistry (IHC)
          beta Galactosidase Antibody in Western Blot (WB)
          beta Galactosidase Antibody in Western Blot (WB)
          beta Galactosidase Antibody in Western Blot (WB)
          beta Galactosidase Antibody in Western Blot (WB)
          beta Galactosidase Antibody in Western Blot (WB)
          beta Galactosidase Antibody in Western Blot (WB)
          beta Galactosidase Antibody in Western Blot (WB)

          Product Details

          A-11132

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          Assay-dependent
          View 13 publications 13 publications

          Immunohistochemistry (IHC)

          Assay-dependent
          View 66 publications 66 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          -
          View 8 publications 8 publications

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 6 publications 6 publications

          Immunocytochemistry (ICC/IF)

          Assay-dependent
          View 13 publications 13 publications

          ELISA (ELISA)

          Assay-dependent
          View 1 publication 1 publication

          ChIP assay (ChIP)

          -
          View 2 publications 2 publications

          Miscellaneous PubMed (MISC)

          -
          View 2 publications 2 publications
          Product Specifications

          Species Reactivity

          Bacteria

          Published species

          Bacteria, Fruit fly, Hamster, Human, Mouse, Rat, Tag

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          beta-Galactosidase

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          2 mg/mL

          Purification

          IgG fraction

          Storage buffer

          PBS, pH 7.2

          Contains

          5mM sodium azide

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_221539

          Product Specific Information

          The E. coli gene lacZ encoding beta-galactosidase is an important reporter gene for detecting the expression of recombinant genes in animal cells. In situations where enzymatic detection of beta-galactosidase is not practical, anti-beta-galactosidase antibody has been widely used. Anti-beta-galactosidase antibody can also be used for purification of fusion proteins by immunoprecipitation or immunoadsorption. Furthermore, anti-beta-galactosidase antibody has been shown to be the preferred analytical tool for light microscopic evaluation of lacZ gene transfer, and the beta-galactosidase/anti-beta-galactosidase complex has been used as a model to assess the feasibility of a high-pressure immunodesorption process.

          This antibody, raised in rabbit against E. coli beta-galactosidase, demonstrates high specificity for the enzyme and is suitable for immunological methods that require high titer and specificity such as immunoblotting (western or dot blot), ELISA, immunoprecipitation, and immunohistochemistry. The appropriate dilution of antibody for a particular application should be determined empirically. We suggest trying dilutions of about 1:5,000 to 1:50,000 for your initial experiments.

          Target Information

          Beta Galactosidase encodes a member of the glycosyl hydrolase 35 family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature lysosomal enzyme. This enzyme catalyzes the hydrolysis of a terminal beta-linked galactose residue from ganglioside substrates and other glycoconjugates. Mutations in this gene have been associated with GM1-gangliosidosis and Morquio B syndrome. Diseases associated with GLB1 include Gm1-Gangliosidosis, Type I and Gm1-Gangliosidosis, Type Ii.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          It has to be done as per old AB suggested Products section.
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