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Invitrogen
beta Lactamase Monoclonal Antibody (8A5.A10)
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FIGURE: 1 / 1
beta Lactamase Antibody (MA1-20370) in WB
Molecular microbiology 2022 -
1 FIGURE T3S of a beta-lactamase reporter by appending the YscX N-terminus. Derivatives of pMMB208 contained yscX-bla translational fusions placed under control of an IPTG inducible promoter. These constructs were maintained in parental (YPIII/pIB102) and Delta sctU null mutant (YPIII/pIB75) bacteria. Overnight cultures of these bacteria were sub-cultured into BHI broth lacking calcium and then grown at 26d... View More
Product Details
MA1-20370
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bacteria
Published species
Bacteria,
Yeast
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
8A5.A10
Immunogen
5’-His-tagged E. coli 205 TEM-1 Rand beta-lactamase.
Conjugate
Form
Liquid
Concentration
1.8 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2280996
Product Specific Information
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Sequence: MSIQHFRVAL IPFFAAFCLP VFAHPETLVK VKDAEDQLGA RVGYIELDLN SGKILESFRP EERFPMMSTF KVLLCGAVLS RVDAGQEQLG RRIHYSQNDL VEYSPVTEKH LTDGMTVREL CSAAITMSDN TAANLLLTTI GGPKELTAFL HNMGDHVTRL DRWEPELNEA IPNDERDTTM PAAMATTLRK LLTGELLTLA SRQQLIDWME ADKVAGPLLR SALPAGWFIA DKSGAGERGS RGIIAALGPD GKPSRIVVIY TTGSQATMDE RNRQIAEIGA SLIKHW
Target Information
The beta lactam antibiotics (penicillins and cephalosporins) are the most frequently used antimicrobial agents. All of the beta lactams are structurally related through the presence of a core beta lactam ring. Bacterial resistance to beta lactams continues to increase, primarily due to the production of beta lactamases. Beta lactamases catalyze the hydrolysis of the beta lactam bond, which destroys antibacterial activity. Bacteria that produce TEM type or SHV type beta lactamases have point mutations in structural genes that have extended the substrate specificity of these beta lactamases. As a result, many of the beta lactamase producing Gram negative pathogens have become multidrug resistant.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: blaCTX-M-14; FLJ14902; G24; MRPL56
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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