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beta TrCP2 Antibody (600-401-483) in WB
Western blot using Rocklands affinity purified anti-bTrCP2 antibody shows detection of mouse and human bTrCP2 (arrowhead) in NIH3T3 (lane 1) and 293 (lane 2) whole cell lysates, respectively. The band appears as a 58 kDa protein, although a 62.1 kDa band is predicted. The identity of faint higher molecular weight bands is not known. The primary antibody was used at a 1:200 dilution incubated in 5% BLOTTO overnight at 4°C. Detection occurred using HRP conjugated Goat-anti-Rabbit IgG (p/n 611-103-122) diluted 1:20,000 in blocking buffer (p/n... View More
Product Details
600-401-483
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Chicken,
Human,
Mouse,
Non-human primate
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the N-terminal of human ßTrCP2 protein.
Conjugate
Form
Liquid
Concentration
0.8 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This antibody reacts with human betaTrCP2 protein. A BLAST analysis was used to suggest cross-reactivity with betaTrCP2 from human, mouse, dog, bovine, chimpanzee, chicken and horse based on a 100% homology with the immunizing sequence. Cross-reactivity with betaTrCP2 from other sources has not been determined.
Target Information
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class and, in addition to an F-box, contains multiple WD40 repeats. This gene contains at least 14 exons, and its alternative splicing generates 3 transcript variants diverging at the presence/absence of two alternate exons.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: beta-transducin repeat-containing protein 2; F-box and WD repeats protein beta-TrCP2; F-box and WD-40 domain protein 11; F-box and WD-40 domain protein 1B; F-box protein Fbw1b; F-box/WD repeat-containing protein 11; F-box/WD repeat-containing protein 1B; Homologous to Slimb protein; HOS
Gene Aliases: 2310065A07Rik; AA536858; BTRC2; BTRCP2; Fbw11; FBW1B; FBXW11; FBXW1B; HOS; KIAA0696; RCJMB04_34f17
UniProt ID: (Human) Q9UKB1, (Mouse) Q5SRY7
Entrez Gene ID: (Chicken) 416181, (Dog) 479291, (Human) 23291, (Mouse) 103583
protein polyubiquitination
protein dephosphorylation
cell cycle
Wnt signaling pathway
protein ubiquitination
SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
protein destabilization
negative regulation of NF-kappaB import into nucleus
positive regulation of circadian rhythm
proteasome-mediated ubiquitin-dependent protein catabolic process
positive regulation of proteolysis
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
rhythmic process
G2/M transition of mitotic cell cycle
stimulatory C-type lectin receptor signaling pathway
NIK/NF-kappaB signaling
Fc-epsilon receptor signaling pathway
T cell receptor signaling pathway
stress-activated MAPK cascade
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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