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beta Tubulin 2 Antibody (PA5-27108) in ICC/IF
Immunofluorescence analysis of beta Tubulin 2 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Beta Tubulin 2 Rabbit Polyclonal Antibody (Product # PA5-27108) at 2 µg/mL in 0.1% BSA and incubated for ... View More
Product Details
PA5-27108
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Fruit fly,
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human beta Tubulin 2. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2544584
Product Specific Information
Recommended positive controls: Jurkat, Raji, NCI-H929, U87-MG, SK-N-SH, IMR32, SK-N-AS, C8D30, NIH3T3, BCL-1, Raw 264.7, C2Cl2, Drosophila lysate , rat brain.
Predicted reactivity: Mouse (100%), Rat (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: b2 tubulin; beta 2 tubulin; beta tubulin; beta(2)Tubulin; Beta-2-tubulin; beta-Tubulin; beta-Tubulin 85D; beta-Tubulin at 85D; beta-tubulin T beta15 (aa 1-445); beta-tubulin85D; beta2 tubulin; beta2-tubulin; beta2tubulin; betaTub85D-PA; betaTubulin85D; CG9359-PA; class II beta-tubulin isotype; class IIa beta-tubulin; class IIb beta-tubulin; dJ40E16.7; T beta-15; testis-specific beta-tubulin; tubb2a {ECO:0000250|UniProtKB:Q7TMM9}; tubulin; tubulin beta; Tubulin beta class IIa; Tubulin beta-2 chain; Tubulin beta-2A chain; Tubulin beta-2B chain; tubulin, beta 2; tubulin, beta 2A class IIa; tubulin, beta 2B class IIb; tubulin, beta polypeptide; tubulin, beta polypeptide 2; tubulin, beta polypeptide paralog
Gene Aliases: 2410129E14Rik; 2t; B2t; bA506K6.1; BETA 85D; beta(2)Tu; beta(2)Tub; beta-tub; beta-Tub85D; BETA2; beta2-tub; beta2t; beta2tub; beta85D; beta;Tub85D; beta[[2]]-tubulin; betaTub2; betaTub85D; brdp; CDCBM5; CG9359; D.m.BETA-85D; Dmbeta2; Dmel\CG9359; Dmel_CG9359; DTB4; M(beta)2; ms(3)KK[D]; PMGYSA; RGD1309427; Tub; TUBB; TUBB2; TUBB2A; TUBB2B; TubB85D
UniProt ID: (Human) Q13885, (Human) Q9BVA1, (Rat) P85108, (Mouse) Q7TMM9, (Mouse) Q9CWF2, (Rat) Q3KRE8, (Fruit fly) P61857
Entrez Gene ID: (Human) 7280, (Human) 347733, (Rat) 498736, (Mouse) 22151, (Mouse) 73710, (Rat) 291081, (Fruit fly) 41124
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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