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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • beta Tubulin Antibodies

          Invitrogen

          beta Tubulin Monoclonal Antibody (2 28 33)

          26 Published Figures
          103 References
          View all (69) beta Tubulin antibodies

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          Datasheet
          Protocols
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          Datasheet
          Protocols
          Questions & Answers

          Cite beta Tubulin Monoclonal Antibody (2 28 33)

          • Antibody Testing Data (8)
          • Published Figures (26)
          beta Tubulin Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          beta Tubulin Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 34

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          beta Tubulin Antibody (32-2600) in ICC/IF

          Visualizing a rat cortical neuron culture. Cryopreserved Gibco™ Primary Mouse Cortical Neurons (Product # A15585) were grown in culture for 3 weeks using the Gibco™ B-27™ Plus Neuronal Culture System (Product # A3653401). Cells were fixed and labeled with Invitrogen™ NucBlue™ Fixed Cell ReadyProbes™ Reagent (Product # R37606), anti-β-3 tubulin mouse monoclonal antibody (Product # 32-2600) in conjunction wit... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          beta Tubulin Antibody in Immunocytochemistry (ICC/IF)
          beta Tubulin Antibody in Immunocytochemistry (ICC/IF)
          beta Tubulin Antibody in Immunocytochemistry (ICC/IF)
          beta Tubulin Antibody in Immunocytochemistry (ICC/IF)
          beta Tubulin Antibody in Immunocytochemistry (ICC/IF)
          beta Tubulin Antibody in Immunocytochemistry (ICC/IF)
          beta Tubulin Antibody in Western Blot (WB)
          beta Tubulin Antibody in Western Blot (WB)
          beta Tubulin Antibody in Immunocytochemistry (ICC/IF)
          beta Tubulin Antibody in Immunocytochemistry (ICC/IF)
          beta Tubulin Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          beta Tubulin Antibody in Immunohistochemistry (IHC)
          beta Tubulin Antibody in Western Blot (WB)
          beta Tubulin Antibody in Western Blot (WB)
          beta Tubulin Antibody in Western Blot (WB)
          beta Tubulin Antibody in Western Blot (WB)
          beta Tubulin Antibody in Western Blot (WB)
          beta Tubulin Antibody in Western Blot (WB)
          beta Tubulin Antibody in Western Blot (WB)
          beta Tubulin Antibody in Western Blot (WB)
          beta Tubulin Antibody in Western Blot (WB)
          beta Tubulin Antibody in Western Blot (WB)
          beta Tubulin Antibody in Western Blot (WB)
          beta Tubulin Antibody in Western Blot (WB)
          beta Tubulin Antibody in Western Blot (WB)
          beta Tubulin Antibody in Western Blot (WB)
          beta Tubulin Antibody in Western Blot (WB)
          beta Tubulin Antibody in Western Blot (WB)
          beta Tubulin Antibody in Western Blot (WB)
          beta Tubulin Antibody in Western Blot (WB)
          beta Tubulin Antibody in Western Blot (WB)
          beta Tubulin Antibody in Western Blot (WB)
          beta Tubulin Antibody in Western Blot (WB)
          beta Tubulin Antibody in Western Blot (WB)

          Product Details

          32-2600

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1-3 µg/mL
          View 74 publications 74 publications

          Immunohistochemistry (IHC)

          -
          View 4 publications 4 publications

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 1 publication 1 publication

          Immunohistochemistry - Free Floating (IHC (Free))

          -
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          1-3 µg/mL
          View 14 publications 14 publications

          Flow Cytometry (Flow)

          -
          View 1 publication 1 publication

          Immunoprecipitation (IP)

          -
          View 2 publications 2 publications

          Miscellaneous PubMed (MISC)

          -
          View 6 publications 6 publications
          Product Specifications

          Species Reactivity

          Dog, C. elegans, Human, Mouse, Non-human primate, Rat

          Published species

          C. elegans, Dog, Human, Mouse, Non-human primate, Pig, Rat

          Host/Isotype

          Mouse / IgG1, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          2 28 33

          Immunogen

          Beta-tubulin from sea urchin (S. purpuratus) sperm.

          Conjugate

          Unconjugated Unconjugated Unconjugated
          • Alexa Fluor 488 
          • Alexa Fluor 555 
          • Alexa Fluor 647 
          • Request custom conjugation

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Protein A

          Storage buffer

          PBS, pH 7.4

          Contains

          0.1% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2533072

          Product Specific Information

          This antibody reacts with the ~50 kDa beta-tubulin and has been shown to bind to the two major and one of the minor beta-tubulin isotypes. Reactivity has been confirmed with mouse NIH3T3 fibroblast cells, rat brain, and mouse testis.

          Target Information

          Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Beta-III tubulin is primarily expressed in neurons and may be involved in neurogenesis, axon guidance and maintenance. Mutations in the beta tubulin gene are the cause of congenital fibrosis of the extraocular muscles type 3. Beta-III tubulin was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues. Glutamate residues at the C-terminus of beta III tubulin can be glutamylated. The precise function of such modifications is unclear. Tubulin is phosphorylated on Ser-172 by CDK1 during cell cycle progression. Ser-172 phosphorylation inhibits tubulin incorporation into microtubules.Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55 kDa subunits: alpha and Beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. Because beta-tubulin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: beta Ib tubulin; beta tubulin 1; beta-4 tubulin; beta-tubulin T beta15 (aa 1-445); betaIII-tubulin; class I beta-tubulin; class II beta-tubulin isotype; class IIa beta-tubulin; class IIb beta-tubulin; class III beta-tubulin; class IVa beta-tubulin; class V beta-tubulin; dJ40E16.7; dystonia 4, torsion (autosomal dominant); HSA10p15 beta-tubulin 4Q; M40 antibody; Neuron-specific class III beta-tubulin; T beta-15; tubb2a {ECO:0000250|UniProtKB:Q7TMM9}; Tubulin 5 beta; tubulin beta; Tubulin beta 8 class VIII; Tubulin beta chain; Tubulin beta class IIa; Tubulin beta class V; tubulin beta MGC4083; Tubulin beta-1 chain; Tubulin beta-2A chain; Tubulin beta-2B chain; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-4A chain; Tubulin beta-5 chain; Tubulin beta-6 chain; Tubulin beta-8 chain; Tubulin beta-III; tubulin, beta 1 class VI; tubulin, beta 2; tubulin, beta 2A class IIa; tubulin, beta 2B class IIb; tubulin, beta 3 class III; tubulin, beta 4; tubulin, beta 4 class IVa; tubulin, beta 4A class IVa; tubulin, beta 6 class V; tubulin, beta class I; tubulin, beta polypeptide; tubulin, beta polypeptide 2; tubulin, beta polypeptide paralog; tubulin, beta, 5

          View more View less

          Gene Aliases: 2310057H16Rik; 2410129E14Rik; 2810484G07Rik; 3200002H15Rik; AA408537; AI325297; AI596182; B130022C14Rik; bA506K6.1; bA631M21.2; BB220206; beta-4; beta-5; brdp; CDCBM; CDCBM1; CDCBM5; CDCBM6; CFEOM3; CFEOM3A; CSCSC1; DYT4; FEOM3; HsT1601; M(beta)1; M(beta)2; M(beta)3; M(beta)4; M(beta)5; M(beta)6; M40; OK/SW-cl.56; OOMD; OOMD2; PMGYSA; RGD1305887; RGD1309427; TUBB; TUBB-5; TUBB1; TUBB2; TUBB2A; TUBB2B; TUBB3; TUBB4; TUBB4A; TUBB5; TUBB6; TUBB8

          View more View less

          UniProt ID: (Human) P07437, (Human) Q9H4B7, (Human) Q13885, (Human) Q9BVA1, (Human) Q13509, (Human) P04350, (Human) Q9BUF5, (Human) Q3ZCM7, (Mouse) A2AQ07, (Rat) P85108, (Mouse) Q7TMM9, (Mouse) Q9CWF2, (Rat) Q3KRE8, (Mouse) Q9ERD7, (Rat) Q4QRB4, (Mouse) Q9D6F9, (Mouse) P99024, (Rat) P69897, (Mouse) Q922F4

          View more View less

          Entrez Gene ID: (Dog) 478702, (Dog) 474830, (Human) 203068, (Human) 81027, (Dog) 485950, (Human) 7280, (Human) 347733, (Dog) 102154194, (Human) 10381, (Dog) 100855815, (Human) 10382, (Human) 84617, (Dog) 480213, (Human) 347688, (Mouse) 545486, (Rat) 679312, (Rat) 498736, (Mouse) 22151, (Mouse) 73710, (Rat) 291081, (Mouse) 22152, (Rat) 246118, (Rat) 29213, (Mouse) 22153, (Mouse) 22154, (Rat) 29214, (Mouse) 67951, (Rat) 307351

          View more View less

          Function(s)
          GTPase activity structural molecule activity structural constituent of cytoskeleton protein binding GTP binding protein domain specific binding ubiquitin protein ligase binding protein complex binding GTPase activating protein binding MHC class I protein binding peptide binding molecular_function nucleotide binding
          Process(es)
          microtubule-based process metabolic process protein polymerization neuron migration mitotic nuclear division axon guidance neuron differentiation G2/M transition of mitotic cell cycle cytoskeleton organization spindle assembly cellular process movement of cell or subcellular component cytoskeleton-dependent intracellular transport natural killer cell mediated cytotoxicity cell division biological_process cell projection organization
          It has to be done as per old AB suggested Products section.
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