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betacellulin Antibody (500-P254-1MG) in WB
Western Blot: To detect Human Betacellulin by Western Blot analysis betacellulin Polyclonal Antibody (Product # 500-P254-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Betacellulin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P254-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human Betacellulin
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930086
Product Specific Information
AA Sequence of recombinant protein: DGNSTRSPET NGLLCGDPEE NCAATTTQSK RKGHFSRCPK QYKHYCIKGR CRFVVAEQTP SCVCDEGYIG ARCERVDLFY.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Betacellulin. Anti-Human Betacellulin-specific antibody was purified by affinity chromatography employing an immobilized Human Betacellulin matrix.
Sandwich ELISA: To detect Human Betacellulin by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human Betacellulin (500-P254Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Betacellulin.
Western Blot: To detect Human Betacellulin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Betacellulin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
The protein encoded by this gene is a member of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: OTTHUMP00000160600; OTTHUMP00000219057; Probetacellulin
Gene Aliases: BTC
UniProt ID: (Human) P35070
Entrez Gene ID: (Human) 685
MAPK cascade
epidermal growth factor receptor signaling pathway
positive regulation of cell proliferation
regulation of phosphatidylinositol 3-kinase signaling
peptidyl-tyrosine phosphorylation
positive regulation of urine volume
ERBB2 signaling pathway
negative regulation of apoptotic process
positive regulation of GTPase activity
positive regulation of cell differentiation
positive regulation of mitotic nuclear division
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
positive regulation of fibroblast proliferation
positive regulation of cell division
regulation of cell motility
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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