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Invitrogen
brat Polyclonal Antibody
FIGURE: 1 / 1
brat Antibody (PA5-144542) in WB
Western blot analysis of brat in recombinant protein using a dilution of 1:1,000. Samples were incubated with brat polyclonal antibody (Product # PA5-144542). Secondary: Goat polyclonal to rabbit IgG at 1:50,000 dilution. Predicted band size: 31 kDa. Observed band size: 31 kDa.
Product Details
PA5-144542
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Fruit fly
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) brat protein (294-487aa)
Conjugate
Form
Liquid
Concentration
5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% Proclin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3092389
Target Information
A NHL-domain family protein that functions a translational repressor to inhibit cell proliferation (PubMed:11274060). Plays a central role in translation repression of hb mRNA by being recruited by nanos (nos) and pum to the Nanos Response Element (NRE), a 16 bp sequence in the hb mRNA 3'-UTR (PubMed:11274060). Probably recruited by other proteins to repress translation of other mRNAs in other tissues (PubMed:11274060). Involved in the regulation of ribosomal RNA synthesis and cell growth (PubMed:11807032). Participates in abdominal segmentation and imaginal disk development (PubMed:11274060). During neuroblast division, segregates asymmetrically and inhibits self-renewal of one of the two daughter cells (PubMed:16564014). Together with the asymmetrically segregating transcription factor prospero ensures that the daughter cell will stop growing, exit the cell cycle, and differentiate into neurons possibly by modulating the function of dm in ganglion mother cells (GMC) (PubMed:11807032, PubMed:16564014). Restricts developmental potential of type II intermediary neuronal progenitor (INP) cells playing a role in proliferation and maturation of the neuroblasts (PubMed:24550111, PubMed:14561773, PubMed:22143802, PubMed:18342578).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: brain tumor; Brain tumor protein brat CG10719; brain tumour; brat-PA; brat-PB; brat-PC; brat-PE; brat-PF; CG10719-PA; CG10719-PB; CG10719-PC; CG10719-PE; CG10719-PF; Protein brain tumor
Gene Aliases: anon-37CDa; BRAT; Cf; CG10719; Dmel\CG10719; Dmel_CG10719; E60; fs(2)ltoPM43; l(2)37Cf; l(2)brat; l(2)E60; lincRNA.S1565
UniProt ID: (Fruit fly) Q8MQJ9
Entrez Gene ID: (Fruit fly) 35197
regulation of translation
phagocytosis
neuroblast fate determination
ganglion mother cell fate determination
neuroblast proliferation
negative regulation of neuroblast proliferation
axon guidance
ventral cord development
brain development
negative regulation of cell proliferation
asymmetric cell division
regulation of synaptic growth at neuromuscular junction
rRNA transcription
rRNA metabolic process
neurogenesis
neuron differentiation
segmentation
oogenesis
regulation of neurogenesis
regulation of cell cycle
asymmetric neuroblast division resulting in ganglion mother cell formation
regulation of synaptic vesicle endocytosis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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